Typical Testing Data/Standard Curve (for reference only)
Cathepsin E Assay Kit | CTSE assay kit
Cathepsin E Inhibitor Screening Kit (Fluorometric)
High-throughput adaptable assay to screen/study/characterize the potential inhibitors of Cathepsin E
Cathepsin E inhibitor is included in the kit
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Background: Cathepsin E (CTSE, EC: 3.4.23.34) is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that is found in highest concentration on the surface of epithelial mucus-producing cells of the stomach. It is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of the gastric cancers. MyBioSource's Cathepsin E Inhibitor Screening Kit utilizes the ability of Cathepsin E to cleave a synthetic MCA-based peptide substrate to release free MCA, which can be easily quantified using a fluorometer or fluorescence microplate reader. In the presence of a Cathepsin E-specific inhibitor, the cleavage of the substrate is reduced/abolished resulting in decrease or total loss of the MCA fluorescence. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize the potential inhibitors of Cathepsin E.
Summary: Detection method-Fluorescence (Ex/Em = 320/420 nm)
Applications- Screen/study/characterize potential inhibitors of Cathepsin E
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease, which is a member of the peptidase C1 family, has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that does not appear to be involved in the digestion of dietary protein and is found in highest concentration in the surface of epithelial mucus-producing cells of the stomach. It is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers. It appears, therefore, to be an oncofetal antigen. Transcript variants utilizing alternative polyadenylation signals and two transcript variants encoding different isoforms exist for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
CTSE: May have a role in immune function. Probably involved in the processing of antigenic peptides during MHC class II-mediated antigen presentation. May play a role in activation-induced lymphocyte depletion in the thymus, and in neuronal degeneration and glial cell activation in the brain. Belongs to the peptidase A1 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Protease; EC 3.4.23.34
Chromosomal Location of Human Ortholog: 1q31
Cellular Component: endosome
Molecular Function: protein homodimerization activity; aspartic-type endopeptidase activity
Biological Process: protein autoprocessing; digestion; antigen processing and presentation of exogenous peptide antigen via MHC class II; proteolysis
Research Articles on CTSE
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Product Notes
The CTSE ctse (Catalog #AAA841466) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Screen/study/characterize potential inhibitors of Cathepsin E. Researchers should empirically determine the suitability of the CTSE ctse for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cathepsin E, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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