Typical Testing Data/Standard Curve (for reference only)
Enteropeptidase/Enterokinase Cleavage Kit Assay Kit
Enteropeptidase / Enterokinase Cleavage Kit
Synonyms
Enteropeptidase/Enterokinase Cleavage Kit; Enteropeptidase / Enterokinase Cleavage Kit; Enteropeptidase/Enterokinase Cleavage Kit assay kit
Features & Benefits
Efficiently removes tags from recombinant fusion proteins containing accessible Enteropeptidase-specific recognition sequence.
Includes cleavage control protein (hPRL-Trx)
Includes cleavage control protein (hPRL-Trx)
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Enteropeptidase/Enterokinase Cleavage Kit assay kit
Product Information: Enteropeptidase/Enterokinase Cleavage Kit: Convenient tool to efficiently remove tags from recombinant fusion proteins containing accessible enteropeptidase-specific recognition sequence. Sufficient for cleaving at least 5 mg of target protein.
Background: Enteropeptidase (Enterokinase, EC 3.4.21.9) is a serine protease involved in activation of trypsinogen to trypsin, which in turn results in the activation of various digestive enzymes. It recognizes a highly specific amino acid sequence 'DDDDK' and cleaves after the lysine residue (K). The high specific activity of Enteropeptidase has been utilized in cleaving a variety of native or fusion proteins containing the above recognition motif. MyBioSource's Enteropeptidase Cleavage Kit contains highly active light chain fragment of human Enteropeptidase. Our pure enzyme is an excellent tool to obtain a wild type protein sequence from a fusion protein, containing Enteropeptidase recognition sequence (DDDDK). This Enteropeptidase cleavage kit is sufficient for cleaving at least 5 mg of target protein. The residual enteropeptidase left in the reaction mix will not interfere with most of the downstream applications. Following cleavage of the target protein, Enteropeptidase can be removed using MyBioSource Hi-Bind Ni-QR Agarose beads.
Summary: Sufficient for cleaving at least 5 mg of target protein
Background: Enteropeptidase (Enterokinase, EC 3.4.21.9) is a serine protease involved in activation of trypsinogen to trypsin, which in turn results in the activation of various digestive enzymes. It recognizes a highly specific amino acid sequence 'DDDDK' and cleaves after the lysine residue (K). The high specific activity of Enteropeptidase has been utilized in cleaving a variety of native or fusion proteins containing the above recognition motif. MyBioSource's Enteropeptidase Cleavage Kit contains highly active light chain fragment of human Enteropeptidase. Our pure enzyme is an excellent tool to obtain a wild type protein sequence from a fusion protein, containing Enteropeptidase recognition sequence (DDDDK). This Enteropeptidase cleavage kit is sufficient for cleaving at least 5 mg of target protein. The residual enteropeptidase left in the reaction mix will not interfere with most of the downstream applications. Following cleavage of the target protein, Enteropeptidase can be removed using MyBioSource Hi-Bind Ni-QR Agarose beads.
Summary: Sufficient for cleaving at least 5 mg of target protein
Product Categories/Family for Enteropeptidase/Enterokinase Cleavage Kit assay kit
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Product Notes
The Enteropeptidase/Enterokinase Cleavage Kit (Catalog #AAA841587) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Enteropeptidase/Enterokinase Cleavage Kit, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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