Typical Testing Data/Standard Curve (for reference only)
SIRT2 Inhibitor Screening Assay Kit
SIRT2 Inhibitor Screening Assay Kit (Fluorometric)
Synonyms
SIRT2 Inhibitor Screening; SIRT2 Inhibitor Screening Assay Kit (Fluorometric); Sirtuin 2 Inhibitor Screening Assay Kit; SIR2-like protein 2 Inhibitor Screening Assay Kit; NAD-dependent protein deacetylase sirtuin-2 Inhibitor Screening Assay Kit; SIRT2 Inhibitor Screening assay kit
Features & Benefits
Simple procedure
Fast and convenient
The assay is sensitive, reliable, and high-throughput adaptable.
Fast and convenient
The assay is sensitive, reliable, and high-throughput adaptable.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SIRT2 Inhibitor Screening assay kit
Product Information: SIRT2 Inhibitor Screening Assay Kit: High-Throughput Adaptable Fluorometric Assay Kit to Screen SIRT2 Inhibitors. Rapid, Simple & Sensitive. Includes Inhibitor Control, Nicotinamide. 100 Assays.
Background: Sirtuin or Sir2 proteins are a class of proteins that possess either histone deacetylase or mono-ribosyltransferase activity. Sirtuins have been implicated in influencing aging and regulating transcription, apoptosis and stress resistance, as well as energy efficiency and alertness during low-calorie situations. Unlike other known protein deacetylases, which simply hydrolyze acetyl-lysine residues, the sirtuin-mediated deacetylation reaction couples lysine deacetylation to NAD hydrolysis. This hydrolysis yields O-acetyl-ADP-ribose, the deacetylated substrate and nicotinamide, itself an inhibitor of sirtuin activity. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. In MyBioSource's Sirtuin inhibitor screening Kit, Sirtuin deacetylates the substrate and then the developer cleaves the deacetylated substrate to release the fluorescent group, which can be detected fluorometrically at Ex/Em = 395/541 nm. In the presence of SIRT inhibitor, the deacetylation will be impeded which prevents the cleavage of the substrate to release the fluorescent group. The kit provides a rapid, simple, sensitive, and reliable test, which is also suitable for high-throughput screening of SIRT2 inhibitors. Inhibitor control (Nicotinamide) is included to compare the efficacy of the test inhibitors.
Summary: Detection method- Fluorescence (Ex/Em 395/541 nm)
Species reactivity- Mammalian
Application-
Screening and characterization of Sirtuin inhibitors.
Background: Sirtuin or Sir2 proteins are a class of proteins that possess either histone deacetylase or mono-ribosyltransferase activity. Sirtuins have been implicated in influencing aging and regulating transcription, apoptosis and stress resistance, as well as energy efficiency and alertness during low-calorie situations. Unlike other known protein deacetylases, which simply hydrolyze acetyl-lysine residues, the sirtuin-mediated deacetylation reaction couples lysine deacetylation to NAD hydrolysis. This hydrolysis yields O-acetyl-ADP-ribose, the deacetylated substrate and nicotinamide, itself an inhibitor of sirtuin activity. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. In MyBioSource's Sirtuin inhibitor screening Kit, Sirtuin deacetylates the substrate and then the developer cleaves the deacetylated substrate to release the fluorescent group, which can be detected fluorometrically at Ex/Em = 395/541 nm. In the presence of SIRT inhibitor, the deacetylation will be impeded which prevents the cleavage of the substrate to release the fluorescent group. The kit provides a rapid, simple, sensitive, and reliable test, which is also suitable for high-throughput screening of SIRT2 inhibitors. Inhibitor control (Nicotinamide) is included to compare the efficacy of the test inhibitors.
Summary: Detection method- Fluorescence (Ex/Em 395/541 nm)
Species reactivity- Mammalian
Application-
Screening and characterization of Sirtuin inhibitors.
Product Categories/Family for SIRT2 Inhibitor Screening assay kit
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Product Notes
The SIRT2 Inhibitor Screening (Catalog #AAA841747) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "SIRT2 Inhibitor Screening, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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