Typical Testing Data/Standard Curve (for reference only)
Human Fibronectin ELISA Kit | fn1 elisa kit
Fibronectin (human) ELISA Kit
Gene Names
fn1; fibronectin
Reactivity
Human
Synonyms
Fibronectin; Fibronectin (human) ELISA Kit; fn1 elisa kit
Reactivity
Human
Sequence Length
2481
Application Notes
Application- Quantitative protein detection, establishing normal range
Samples
Serum & plasma (heparin, EDTA, Citrate), Cell Culture Supernatants
Assay Type
Sandwich
Detection Method
Absorbance (450 nm)
Features & Benefits
Easy, convenient and time-saving method to assay for human Fibronectin.
Detection Range:156 pg/ml - 10,000 pg/ml. Sensitivity: < 10 pg/ml.
This ELISA kit shows no cross-reactivity with other relevant proteins.
Detection Range:156 pg/ml - 10,000 pg/ml. Sensitivity: < 10 pg/ml.
This ELISA kit shows no cross-reactivity with other relevant proteins.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for fn1 elisa kit
Product Information: Fibronectin (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human Fibronectin in serum, plasma & cell culture supernatants.Sensitivity: < 10 pg/ml. 100 assays.
Background: Fibronectin is a 430 KDa dimeric glycoprotein that exists in 2 forms - cellular and plasma fibronectin. MyBioSource's human Fibronectin ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A rabbit polyclonal antibody specific for human Fibronectin is coated on a 96-well plate. Standards and test samples are added to the wells and Fibronectin present in a sample is bound by the immobilized antibody. A biotinylated polyclonal antibody from goat specific for Fibronectin is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human Fibronectin captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 156 pg/ml - 10,000 pg/ml. Sensitivity: < 10 pg/ml.
Summary: Detection method- Absorbance (450 nm)
Species reactivity-human
Application- Quantitative protein detection, establishing normal range, validation of antibody array results
Background: Fibronectin is a 430 KDa dimeric glycoprotein that exists in 2 forms - cellular and plasma fibronectin. MyBioSource's human Fibronectin ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A rabbit polyclonal antibody specific for human Fibronectin is coated on a 96-well plate. Standards and test samples are added to the wells and Fibronectin present in a sample is bound by the immobilized antibody. A biotinylated polyclonal antibody from goat specific for Fibronectin is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human Fibronectin captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 156 pg/ml - 10,000 pg/ml. Sensitivity: < 10 pg/ml.
Summary: Detection method- Absorbance (450 nm)
Species reactivity-human
Application- Quantitative protein detection, establishing normal range, validation of antibody array results
Product Categories/Family for fn1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
272,680 Da
NCBI Official Full Name
fibronectin
NCBI Official Synonym Full Names
fibronectin 1<
NCBI Official Symbol
fn1
NCBI Official Synonym Symbols
fibronectin
NCBI Protein Information
fibronectin
UniProt Protein Name
Fibronectin
Protein Family
UniProt Gene Name
fn1
UniProt Synonym Gene Names
FN
UniProt Entry Name
FINC_XENLA
Uniprot Description
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. May be involved in osteoblast compaction ().
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Product Notes
The Human fn1 fn1 (Catalog #AAA843382) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA843382 ELISA Kit recognizes Human fn1. Application- Quantitative protein detection, establishing normal range. Researchers should empirically determine the suitability of the fn1 fn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fibronectin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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