Human TIMP3 ELISA Kit | TIMP3 elisa kit

Human TIMP3 Quick ELISA Kit

Cat. Num. AAA1753921

• Gene Names: TIMP3; SFD; K222; K222TA2; HSMRK222
• Reactivity: Human
• Synonyms: TIMP3; Human TIMP3 Quick ELISA Kit; Metalloproteinase inhibitor 3; Protein MIG-5; Tissue inhibitor of metalloproteinases 3; TIMP-3; TIMP metallopeptidase inhibitor 3; TIMP3 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human TIMP3

• Assay Type: Colorimetric sandwich
• Samples: cell culture supernatants, serum, plasma (heparin. EDTA) and saliva
• Detection Range: 156pg/ml-10,000pg/ml
• Sensitivity: <2pg/ml
• Capture/Detection Antibodies: Capture antibody- monoclonal antibody from mouse|Detection antibody- polyclonal antibody from goat
• Sample Data: Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000; O.D.|0.111|0.160|0.190|0.231|0.414|1.003|2.177|2.388
• Intra-Assay Precision: Sample|1|2|3|; n|16|16|16|; Mean(pg/ml)|228|1364|4394|; Standard deviation|14.59|90.02|237.27|; CV(%)|6.4%|6.6%|5.4%|
• Inter-Assay Precision: Sample|1|2|3|; n|24|24|24; Mean(pg/ml)|244|1487|4066; Standard deviation|19.03|107.06|333.41; CV(%)|7.8%|7.2%|8.2%
• Reproducibility: To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.; Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%); Sample 1: 228|257|260|256|250|11.25|4.5%; Sample 2: 1364|1309|1336|1522|1382|81.53|5.9%; Sample 3: 4394|4601|4288|4589|4468|285.95|6.4%; *number of samples for each test n=16.
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: C24-P211
• Anticoagulant: heparin or EDTA
• Benefits: The Quick ELISA kits, assay takes less than 1.5 hours. Detect Human TIMP3 with <2pg/ml sensitivity. Format: 96-well plate with removable strips.; This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.
• Principle: Human TIMP3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human TIMP3 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for TIMP3. The detection antibody is a HRP linked goat polyclonal antibody specific for TIMP3. The kit contains recombinant Human TIMP3 with immunogen: Expression system for standard: NS0; Immunogen sequence: C24-P211. The kit is analytically validated with ready to use reagents.; ; To measure Human TIMP3, add standards and samples to the wells, then add HRP linked detection antibody. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human TIMP3 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human TIMP3 in the sample.
• Assay Procedure Step 1: 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312.5pg/ml, 156.25pg/ml
• TMB Incubation Time: 15
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice.)

Testing Data

Related Product Information for TIMP3 elisa kit

The tissue inhibitors of metalloproteinases(TIMPs) are natural inhibitors of the matrix metalloproteinases, a group of zinc-binding endopeptidases involved in the degradation of the extracellular matrix. The TIMP3 gene is expressed in many tissues, with highest expression in the placenta. TIMP3 encodes a potent angiogenesis inhibitor and is mutated in Sorsby fundus dystrophy, a macular degenerative disease with submacular choroidal neovascularization. TIMP3 gene is mapped to 22q12. 1-q13. 2. Mutations in TIMP3 cause the autosomal dominant disorder Sorsby's fundus dystrophy(SFD).

Product Categories/Family for TIMP3 elisa kit

ELISA kits

NCBI and Uniprot Product Information

• NCBI GI #: 4507513
• NCBI GeneID: 7078
• NCBI Accession #: NP_000353.1
• NCBI GenBank Nucleotide #: NM_000362.4
• UniProt Accession #: P35625; Q5THV4; Q9UC74; Q9UGS2; B2RBY9
• Molecular Weight: 24,145 Da
• NCBI Official Full Name: metalloproteinase inhibitor 3
• NCBI Official Synonym Full Names: TIMP metallopeptidase inhibitor 3
• NCBI Official Symbol: TIMP3
• NCBI Official Synonym Symbols: SFD; K222; K222TA2; HSMRK222
• NCBI Protein Information: metalloproteinase inhibitor 3; TIMP-3; MIG-5 protein; protein MIG-5; tissue inhibitor of metalloproteinases 3
• UniProt Protein Name: Metalloproteinase inhibitor 3
• Protein Family: Metalloproteinase inhibitor
• UniProt Gene Name: TIMP3
• UniProt Synonym Gene Names: TIMP-3
• UniProt Entry Name: TIMP3_HUMAN

NCBI Description

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy. [provided by RefSeq, Jul 2008]

Uniprot Description

TIMP3: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15. Interacts with EFEMP1. Belongs to the protease inhibitor I35 (TIMP) family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 22q12.3

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; basement membrane; nucleus

Molecular Function: metalloendopeptidase inhibitor activity; protein binding; protease binding; metal ion binding

Biological Process: negative regulation of metalloenzyme activity; visual perception; response to cytokine stimulus; response to hormone stimulus; negative regulation of membrane protein ectodomain proteolysis

Disease: Fundus Dystrophy, Pseudoinflammatory, Of Sorsby