Typical Testing Data/Standard Curve (for reference only)
Beta-Secretase Assay Kit
Beta-Secretase Activity Fluorometric Assay Kit
Synonyms
Beta-Secretase; Beta-Secretase Activity Fluorometric Assay Kit; BACE1 Activity Fluorometric Assay Kit; ASP2 Activity Fluorometric Assay Kit; Memapsin-2 Activity Fluorometric Assay Kit; Beta-Secretase assay kit
Samples
Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
Features & Benefits
Simple procedure; takes ~ 1 hour
Fast and convenient
The assay is sensitive, stable and high-throughput adaptable.
Fast and convenient
The assay is sensitive, stable and high-throughput adaptable.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Beta-Secretase assay kit
Product Information: beta-Secretase Activity Assay Kit: convenient Fluorometric Assay for Detecting beta-secretase Activity in Biological and Purified samples. 100 Assays.
Background: beta-Secretase has been implicated to be an excellent target for anti-amyloid therapy for the treatment of Alzheimer's disease. The beta-Secretase activity Assay Kit provides a convenient fluorescence method for detecting beta-secretase activity in biological and purified samples. The assay utilizes a secretase-specific peptide conjugated to two reporter molecules EDANS and DABCYL. In the uncleaved form, the fluorescent emissions from EDANS are quenched by the physical proximity of the DABCYL moiety. Cleavage of the peptide by secretase physically separates EDANS and DABCYL allowing for the release of a fluorescent signal. The level of secretase enzymatic activity in samples is proportional to the level of fluorescence intensity.
Summary: Detection method- Fluorescence (Ex/Em 335-355/ 495-510 nm)
Species reactivity- Mammalian
Applications- Convenient fluorescence method for detecting beta-secretase activity in biological and purified samples.
Background: beta-Secretase has been implicated to be an excellent target for anti-amyloid therapy for the treatment of Alzheimer's disease. The beta-Secretase activity Assay Kit provides a convenient fluorescence method for detecting beta-secretase activity in biological and purified samples. The assay utilizes a secretase-specific peptide conjugated to two reporter molecules EDANS and DABCYL. In the uncleaved form, the fluorescent emissions from EDANS are quenched by the physical proximity of the DABCYL moiety. Cleavage of the peptide by secretase physically separates EDANS and DABCYL allowing for the release of a fluorescent signal. The level of secretase enzymatic activity in samples is proportional to the level of fluorescence intensity.
Summary: Detection method- Fluorescence (Ex/Em 335-355/ 495-510 nm)
Species reactivity- Mammalian
Applications- Convenient fluorescence method for detecting beta-secretase activity in biological and purified samples.
Product Categories/Family for Beta-Secretase assay kit
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Product Notes
The Beta-Secretase (Catalog #AAA841688) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Beta-Secretase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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