Sheep Contactin-1 Polyclonal Antibody | anti-CNTN1 antibody

Contactin-1 (Neural Cell Surface Protein F3, Cntn1)

Cat. Num. AAA6007601

• Gene Names: CNTN1; F11
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Contactin-1; Polyclonal Antibody; Contactin-1 (Neural Cell Surface Protein F3; Cntn1); Anti -Contactin-1 (Neural Cell Surface Protein F3; anti-CNTN1 antibody

• Host: Sheep
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse Contactin-1. Species Crossreactivity: Human and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.

• Applicable Applications for anti-CNTN1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 0.2ug/ml
• Immunogen: Recombinant corresponding to aa21-999 from mouse Contactin-1 expressed in NSO cell line.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Detection of Human, Mouse, and Rat Contactin1 by Western Blot. Western blot shows lysates of human brain (hypothalamus) tissue, mouse brain (cerebellum) tissue, andrat brain tissue. PVDF membrane was probed with 0.2ug/ml of Sheep Human/Mouse/Rat Contactin1Affinity purified Polyclonal followed by HRP-conjugated Sheep IgG Secondary. A specific band was detected for Contactin1 at approximately 135kD (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.)

Related Product Information for anti-CNTN1 antibody

CNTN1 (Contactin1 also neuronal cell surface protein F3/F11) is a 135-145kD GPI-linked glycoprotein member of the Contactin family, Ig superfamily of molecules. It is expressed on the surface of oligodendrocytes and neurons, principally on axons, and has multiple binding partners. In particular, it binds to astrocyte tenascin C, Schwann cell NF/neurofascin155, and oligodendrocyte produced tenascin-R and Notch intrans, plus paranodin in cis. Tenascin-R interactions inhibit neurite outgrowth, paranodin/NF155 interactions may contribute to Ranvier node development, and Notch ligation results in oligodendrocyte maturation. CNTN1 also binds to chondroitin sulfate E, an interaction that initiates neurite outgrowth. Mouse CNTN1 is synthesized as a 1020aa preproprecursor (aa1-1020). It contains a 20aa signal sequence, followed by a 981aa mature region (aa21-1001), and a 19aa propeptide. The mature region possesses six consecutive C2-type Ig-like domains (aa41-603) that regulate neurite outgrowth, followed by three FN-type III domains (aa605-901) that regulate adhesiveness. It is estimated that 15% of the native MW constitutes carbohydrate, part of which is represented by the HNK 1 antigen (3sulfo-GluA-Gal-GlcNac). Over aa20-999, mouse CNTN1 shares 96% and 99% aa sequence identity with human and rat CNTN1, respectively.

Product Categories/Family for anti-CNTN1 antibody

Antibodies; Abs to Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 292494926
• NCBI GeneID: 417786
• NCBI Accession #: NP_001004381.3
• NCBI GenBank Nucleotide #: NM_001004381.3
• UniProt Accession #: P14781; P10450
• Molecular Weight: 112,507 Da
• NCBI Official Full Name: contactin-1 preproprotein
• NCBI Official Synonym Full Names: contactin 1
• NCBI Official Symbol: CNTN1
• NCBI Official Synonym Symbols: F11
• NCBI Protein Information: contactin-1; neural cell recognition protein; neural cell recognition molecule F11
• UniProt Protein Name: Contactin-1
• Protein Family: Contactin
• UniProt Gene Name: CNTN1
• UniProt Entry Name: CNTN1_CHICK

Uniprot Description

Function: Mediates cell surface interactions during nervous system development. Interaction with TNR enhances the neurite outgrowth. Ref.4

Subunit structure: Interacts with TNR. Ref.4

Subcellular location: Cell membrane; Lipid-anchor › GPI-anchor Ref.3.

Sequence similarities: Belongs to the immunoglobulin superfamily. Contactin family.Contains 4 fibronectin type-III domains.Contains 6 Ig-like C2-type (immunoglobulin-like) domains.

Sequence caution: The sequence CAA68753.1 differs from that shown. Reason: Frameshift at positions 1002 and 1007.