Human a1 microglobulin ELISA Kit | A1 MG elisa kit

Human a1 microglobulin ELISA Kit

Cat. Num. AAA729718

• Gene Names: AMBP; A1M; HCP; ITI; UTI; EDC1; HI30; ITIL; IATIL; ITILC
• Reactivity: Human
• Synonyms: a1 microglobulin; Human a1 microglobulin ELISA Kit; A1 MG elisa kit

• Reactivity: Human

• Assay Type: Competitive or Sandwich
• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL.
• Intended Uses: This A1-MG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human A1-MG. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for A1 MG elisa kit

Principle of the Assay: A1-MG ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for A1-MG. Standards or samples are then added to the microtiter plate wells and A1-MG if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of A1-MG present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for A1-MG are added to each well to "sandwich" the A1-MG immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain A1-MG and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The A1-MG concentration in each sample is interpolated from this standard curve.

Product Categories/Family for A1 MG elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 579676
• NCBI GeneID: 259
• UniProt Accession #: P02760; P00977; P02759; P78491; Q2TU33; Q5TBD7; Q9UC58; Q9UDI8
• Molecular Weight: 38,999 Da
• NCBI Official Full Name: alpha-1-microglobulin, partial
• NCBI Official Synonym Full Names: alpha-1-microglobulin/bikunin precursor
• NCBI Official Symbol: AMBP
• NCBI Official Synonym Symbols: A1M; HCP; ITI; UTI; EDC1; HI30; ITIL; IATIL; ITILC
• NCBI Protein Information: protein AMBP; bikunin; uristatin; protein HC; trypstatin; uronic-acid-rich protein; growth-inhibiting protein 19; inter-alpha-trypsin inhibitor light chain; complex-forming glycoprotein heterogeneous in charge
• UniProt Protein Name: Protein AMBP
• UniProt Gene Name: AMBP
• UniProt Synonym Gene Names: HCP; ITIL; Protein HC; ITI-LC
• UniProt Entry Name: AMBP_HUMAN

NCBI Description

This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes. [provided by RefSeq, Jul 2008]

Uniprot Description

AMBP: Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9q32-q33

Cellular Component: extracellular space; cell surface; intracellular membrane-bound organelle; extracellular region; plasma membrane

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; protein homodimerization activity; calcium oxalate binding; IgA binding; heme binding; calcium channel inhibitor activity

Biological Process: negative regulation of JNK cascade; receptor-mediated endocytosis; viral reproduction; heme catabolic process; negative regulation of immune response; protein catabolic process; female pregnancy; cell adhesion; protein-chromophore linkage