SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
G-CSF active protein
G-CSF, 31-204 aa, Human, E Coli (Bioactivity Validated)
Gene Names
CSF3; GCSF; CSF3OS; C17orf33
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
G-CSF; 31-204 aa; Human; E Coli (Bioactivity Validated); Granulocyte-colony stimulating factor; CSF3; GCSF; Pluripoietin; Filgrastim; Lenograstim; Granulocyte colony-stimulating factor; Granulocyte-colony stimulating factor Colony stimulating factor 3 (granulocyte); CSF 3; CSF beta; Csfg; GCSA; Granulocyte colony stimulating factor; Macrophage granulocyte inducer 2; MGC45931; MGI 2; ; G-CSF active protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In phosphate-buffered saline (PBS), pH7.4, 10% glycerol
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MTPLGPASSL PQSFLLKCLE QVRKIQGDGA ALQEKLCATY KLCHPEELVL LGHSLGIPWA PLSSCPSQAL QLAGCLSQLH SGLFLYQGLL QALEGISPEL GPTLDTLQLD VADFATTIWQ QMEELGMAPA LQPTQGAMPA FASAFQRRAG GVLVASHLQS FLEVSYRVLR HLAQP
Sequence Length
204
Applicable Applications for G-CSF active protein
SDS-PAGE, Bioactivity
Domain
31-204aa
Endotoxin Level
< 1.0 EU per 1 ug of protein (determined by LAL method)
Biological Activity
Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is less than or equal to 0.3 ng/ml.
Tag
Non-Tagged
Preparation and Storage
Can be stored at +2C to 8°C for up to 1 week.
For long term storage, aliquot and store at -20°C or -80°C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20°C or -80°C.
Avoid repeated freezing and thawing cycles.
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
Testing Data
(Human G-CSF stimulates cell proliferation of the M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 range )
Testing Data
(Human G-CSF stimulates cell proliferation of the M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 range )
Related Product Information for G-CSF active protein
Human granulocyte colony-stimulating factor (hG-CSF) is one of the hematopoietic growth factors which plays an important role in stimulating proliferation, differentiation, and functional activation of blood cells. In addition to its effect on hematopoiesis, G-CSF also enhances neutrophil functions against bacteria, fungi, and tumor cells mediated by antibody-dependent cell-mediated cytotoxicity. Recombinant hG-CSF was expressed in E Coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Product Categories/Family for G-CSF active protein
References
Nomura H, et al. (1986) EMBO J, 5 (5): 871-6; Nagata, S., et al. (1986) Nature, 319 : 415-418; Demetri, G. D., et al. (1991) Blood, 78: 2791-2808 ; Hill, C. P., et al (1993) Proc. Natl. Acad. Sci. uSA, 90:5167-5171;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18.8kDa (175aa), confirmed by MALDI-TOF.
NCBI Official Full Name
granulocyte colony-stimulating factor isoform b
NCBI Official Synonym Full Names
colony stimulating factor 3
NCBI Official Symbol
CSF3
NCBI Official Synonym Symbols
GCSF; CSF3OS; C17orf33
NCBI Protein Information
granulocyte colony-stimulating factor
UniProt Protein Name
Granulocyte colony-stimulating factor
UniProt Gene Name
CSF3
UniProt Synonym Gene Names
C17orf33; GCSF; G-CSF
NCBI Description
The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]
Uniprot Description
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
Research Articles on G-CSF
Similar Products
Product Notes
The G-CSF csf3 (Catalog #AAA203079) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's G-CSF can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Bioactivity. Researchers should empirically determine the suitability of the G-CSF csf3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MTPLGPASSL PQSFLLKCLE QVRKIQGDGA ALQEKLCATY KLCHPEELVL LGHSLGIPWA PLSSCPSQAL QLAGCLSQLH SGLFLYQGLL QALEGISPEL GPTLDTLQLD VADFATTIWQ QMEELGMAPA LQPTQGAMPA FASAFQRRAG GVLVASHLQS FLEVSYRVLR HLAQP. It is sometimes possible for the material contained within the vial of "G-CSF, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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