M-CSF active protein

Recombinant M-CSF

Cat. Num. AAA201169

• Gene Names: Csf1; op; Csfm; MCSF; C87615
• Applications: SDS-Page
• Purity: > 90% by SDS - PAGE
• Synonyms: M-CSF; Recombinant M-CSF; M-Csf; Macrophage colony-stimulating factor 1; CSF-1; MCSF; Csf1; C87615; op; Processedmacrophage colony-stimulating factor 1; M-CSF active protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS - PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
• Concentration: 1mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 33-187aa
• Sequence: KEVSEHC SHMIGNGHLK VLQQLIDSQM ETSCQIAFEF VDQEQLDDPV CYLKKAFFLV QDIIDETMRF KDNTPNANAT ERLQELSNNL NSCFTKDYEE QNKACVRTFH ETPLQLLEKI KNFFNETKNL LEKDWNIFTK NCNNSFAKCS SRDVVTKP
• Sequence Length: 257

• Applicable Applications for M-CSF active protein: SDS-PAGE; Bioactivity
• Tag: His-Tag
• Biological Activity: Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 4 ng/ml.
• Predicted nterm: Ala
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
• Preparation and Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoidrepeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition andvisualized by coomassie blue stain)

Activity

(Mouse M-CSF stimulates cell proliferation of the M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 range is )

Related Product Information for M-CSF active protein

M-CSF, also known as macrophage colony-stimulating factor 1 isoform 1, is one of the hematopoietic growth factors that regulate the growth and differentiation of blood cells. This protein is produced by monocytes,granulocytes, endothelial cells, and fibroblasts. It stimulates the formation of macrophage colonies, enhancesantibody-dependent, cell-mediated cytotoxicity by monocytes and macrophages, and inhibits bone resorption byosteoclasts. It plays an essential role in the regulation of survival, proliferation and differentiation ofhematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. This proteinpromotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and ininflammatory processes. Recombinant mouse M-CSF, fused to His-tag at C-terminus, was expressed in insect celland purified by using conventional chromatography techniques.

Product Categories/Family for M-CSF active protein

Immunology

References

Richardsen E., et al. (2018) Anticancer Res. 35(2):865-874.;;Braza MS., et al. (2018) Am J Transplant. 18:1247-1255.

NCBI and Uniprot Product Information

• NCBI GI #: 166064046
• NCBI GeneID: 12977
• NCBI Accession #: NP_001107001.1
• NCBI GenBank Nucleotide #: NP_001107001.1
• UniProt Accession #: P07141
• Molecular Weight: 19.1kDa (164aa)
• NCBI Official Full Name: macrophage colony-stimulating factor 1 isoform 2
• NCBI Official Synonym Full Names: colony stimulating factor 1 (macrophage)
• NCBI Official Symbol: Csf1
• NCBI Official Synonym Symbols: op; Csfm; MCSF; C87615
• NCBI Protein Information: macrophage colony-stimulating factor 1
• UniProt Protein Name: Macrophage colony-stimulating factor 1
• UniProt Gene Name: Csf1
• UniProt Synonym Gene Names: Csfm; CSF-1; MCSF
• UniProt Entry Name: CSF1_MOUSE

Uniprot Description

M-CSF: Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. Aberrant expression of CSF1 or CSF1R can promote cancer cell proliferation, invasion and formation of metastases. Overexpression of CSF1 or CSF1R is observed in a significant percentage of breast, ovarian, prostate, and endometrial cancers. Aberrant expression of CSF1 or CSF1R may play a role in inflammatory diseases, such as rheumatoid arthritis, glomerulonephritis, atherosclerosis, and allograft rejection. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cytokine

Cellular Component: extracellular space; membrane; perinuclear region of cytoplasm; extracellular region; plasma membrane; integral to membrane; receptor complex

Molecular Function: protein binding; protein homodimerization activity; growth factor activity; cytokine activity; macrophage colony stimulating factor receptor binding

Biological Process: positive regulation of monocyte differentiation; ossification; macrophage differentiation; immune system process; positive regulation of osteoclast differentiation; positive regulation of cellular protein metabolic process; positive regulation of odontogenesis of dentine-containing teeth; reproductive developmental process; osteoclast differentiation; positive regulation of multicellular organism growth; odontogenesis; cell proliferation; positive regulation of mononuclear cell proliferation; homeostasis of number of cells within a tissue; positive regulation of protein kinase activity; positive regulation of cell proliferation; innate immune response; positive regulation of Ras protein signal transduction; inflammatory response; positive regulation of macrophage differentiation; positive regulation of cell-matrix adhesion; regulation of ossification; positive regulation of cell migration