Rat Protein Kinase C Alpha (PKCa) ELISA Kit | PKCa elisa kit

Rat Protein Kinase C Alpha (PKCa) ELISA Kit

Cat. Num. AAA2022832

• Gene Names: PRKCA; AAG6; PKCA; PRKACA; PKC-alpha
• Reactivity: Rat
• Synonyms: Protein Kinase C Alpha (PKCa); Rat Protein Kinase C Alpha (PKCa) ELISA Kit; PRKCA; PKC-A; AAG6; PKC-Alpha; PKCa elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Protein Kinase C Alpha (PKCa).; No significant cross-reactivity or interference between Protein Kinase C Alpha (PKCa) and analogues was observed.
• Sequence Length: 672

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.117ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Kinase C Alpha (PKCa) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Kinase C Alpha (PKCa) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PKCa elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase C Alpha (PKCa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase C Alpha (PKCa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase C Alpha (PKCa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase C Alpha (PKCa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PKCa elisa kit

Signal transduction; Enzyme & Kinase; Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 15099971
• NCBI GeneID: 5578
• UniProt Accession #: P17252; Q15137; Q32M72; Q96RE4; B5BU22
• Molecular Weight: 76,750 Da
• NCBI Official Full Name: protein kinase C alpha, partial
• NCBI Official Synonym Full Names: protein kinase C, alpha
• NCBI Official Symbol: PRKCA
• NCBI Official Synonym Symbols: AAG6; PKCA; PRKACA; PKC-alpha
• NCBI Protein Information: protein kinase C alpha type
• UniProt Protein Name: Protein kinase C alpha type
• Protein Family: Protein kinase
• UniProt Gene Name: PRKCA
• UniProt Synonym Gene Names: PKCA; PRKACA; PKC-A; PKC-alpha
• UniProt Entry Name: KPCA_HUMAN

NCBI Description

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq, Jul 2008]

Uniprot Description

PKCA: an AGC kinase of the PKC family. A classical PKC downstream of many mitogenic and receptors. Classical PKCs are calcium-dependent enzymes that are activated by phosphatidylserine, diacylglycerol and phorbol esters. Contains a pseudo-substrate autoinhibitory domain that binds to the catalytic domain preventing its activation in the absence of cofactors or activators.

Protein type: Protein kinase, AGC; EC 2.7.11.13; Protein kinase, Ser/Thr (non-receptor); Oncoprotein; Kinase, protein; AGC group; PKC family; Alpha subfamily

Chromosomal Location of Human Ortholog: 17q22-q23.2

Cellular Component: nucleoplasm; photoreceptor outer segment; cell soma; mitochondrion; endoplasmic reticulum; perinuclear region of cytoplasm; apical part of cell; mitochondrial membrane; dendrite; cytoplasm; plasma membrane; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; protein kinase C activity; enzyme binding; zinc ion binding; calcium-dependent protein kinase C activity; ATP binding; protein kinase activity

Biological Process: phototransduction, visible light; extracellular matrix organization and biogenesis; positive regulation of cell adhesion; nerve growth factor receptor signaling pathway; regulation of muscle contraction; positive regulation of lipopolysaccharide-mediated signaling pathway; mitotic nuclear envelope disassembly; negative regulation of insulin receptor signaling pathway; positive regulation of mitotic cell cycle; signal transduction; protein amino acid phosphorylation; induction of positive chemotaxis; negative regulation of cell proliferation; synaptic transmission; chondrocyte differentiation; angiogenesis; positive regulation of macrophage differentiation; cell adhesion; regulation of peptidyl-tyrosine phosphorylation; regulation of the force of heart contraction; inactivation of MAPK activity; epidermal growth factor receptor signaling pathway; platelet activation; neutrophil chemotaxis; fibroblast growth factor receptor signaling pathway; adenylate cyclase activation; positive regulation of blood vessel endothelial cell migration; negative regulation of adenylate cyclase activity; cellular calcium ion homeostasis; rhodopsin mediated signaling; positive regulation of angiogenesis; negative regulation of glucose import; regulation of rhodopsin mediated signaling; phospholipase C activation; energy reserve metabolic process; innate immune response; positive regulation of endothelial cell proliferation; gene expression; positive regulation of protein amino acid phosphorylation; mitotic cell cycle; vascular endothelial growth factor receptor signaling pathway; blood coagulation; regulation of insulin secretion; positive regulation of inflammatory response; positive regulation of cell migration