SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
IL-1 beta/IL-1F2 Active Protein | IL-1beta/IL-1F2 active protein
Recombinant canine IL-1 beta/IL-1F2 protein
Gene Names
IL1B; IL-1
Applications
SDS-Page
Purity
>95% by SDS-PAGE
Synonyms
IL-1 beta/IL-1F2; Recombinant canine IL-1 beta/IL-1F2 protein; Interleukin 1b; Interleukin 1beta; IL-1; IL1F2; IL1-beta; Interleukin 1; beta proprotein; IL1B; interleukin 1 beta; IL-1B; IL-1beta/IL-1F2 active protein
Host
E Coli
Purity/Purification
>95% by SDS-PAGE
Form/Format
Liquid. In Phosphate-Buffered Saline (pH7.4)
Concentration
1mg/ml (determined by absorbance at 280nm) (varies by lot)
Sequence Positions
114-265aa
Sequence
MAAMQSVDCK LQDISHKYLV LSNSYELRAL HLNGENVNKQ VVFHMSFVHG DESNNKIPVV LGIKQKNLYL SCVMKDGKPT LQLEKVDPKV YPKRKMEKRF VFNKIEIKNT VEFESSQYPN WYISTSQVEG MPVFLGNTRG GQDITDFTME FSS
Applicable Applications for IL-1beta/IL-1F2 active protein
SDS-PAGE, Bioactivity
Endotoxin
<1 EU per 1ug of protein (determined by LAL method)
Species
Canine
Tag
Non-Tagged
Bioactivity
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 range <= 5pg/ml.
Preparation and Storage
Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
Biological Activity
(Canine IL-1B stimulates cell proliferation of the D10.G4.1 mouse helper T cells. The ED50 range range )
Biological Activity
(Canine IL-1B stimulates cell proliferation of the D10.G4.1 mouse helper T cells. The ED50 range range )
Related Product Information for IL-1beta/IL-1F2 active protein
Interleukin-1 (IL-1) has been considered a potentially important inflammatory mediator. It is composed of two distinct proteins, called as IL-1alpha and IL-1beta. IL-1beta is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta bind to the same receptor and they are structurally related polypeptides that share approximately 20% amino acid. Recombinant canine IL-1beta was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for IL-1beta/IL-1F2 active protein
References
Allan SM., et al. (2005), Nat. Rev. Immunol. 5:629; Curr Pharm Des. (2017), 23(32):4893-4905.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,143 Da
NCBI Official Full Name
interleukin-1 beta
NCBI Official Symbol
IL1B
NCBI Official Synonym Symbols
IL-1
NCBI Protein Information
interleukin-1 beta
UniProt Protein Name
Interleukin-1 beta
UniProt Gene Name
IL1B
Uniprot Description
Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production.
Research Articles on IL-1beta/IL-1F2
Similar Products
Product Notes
The IL-1beta/IL-1F2 il1b (Catalog #AAA201269) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 114-265aa. AAA Biotech's IL-1 beta/IL-1F2 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Bioactivity. Researchers should empirically determine the suitability of the IL-1beta/IL-1F2 il1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MAAMQSVDCK LQDISHKYLV LSNSYELRAL HLNGENVNKQ VVFHMSFVHG DESNNKIPVV LGIKQKNLYL SCVMKDGKPT LQLEKVDPKV YPKRKMEKRF VFNKIEIKNT VEFESSQYPN WYISTSQVEG MPVFLGNTRG GQDITDFTME FSS. It is sometimes possible for the material contained within the vial of "IL-1 beta/IL-1F2, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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