GM-CSF active protein

GM-CSF, 18-144 aa, Human, E Coli (Bioactivity Validated)

Cat. Num. AAA203082

• Gene Names: CSF2; CSF; GMCSF
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: GM-CSF; 18-144 aa; Human; E Coli (Bioactivity Validated); Granulocyte-macrophage colony stimulating factor; CSF2; GMCSF; Colony-stimulating factor; CSF; Sargramostim; Molgramostin; Granulocyte-macrophage colony-stimulating factor; Granulocyte-macrophage colony stimulating factor Burst Promoting Activity; CMCSF; Colony stimulating factor; Colony Stimulating Factor 2; CSF 2; CSF Alpha; Eosinophil Colony Stimulating Factor; Granulocyte Macrophage Colony Stimulating Factor; MGC131935; MGC138897; Pluripoietin Alpha; ; GM-CSF active protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In phosphate-buffered saline (PBS), pH 7.4
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE
• Sequence Length: 144

• Applicable Applications for GM-CSF active protein: SDS-PAGE
• Antigen Species: Human
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Biological Activity: The ED50 for this effect is < 0.18 ng/ml. Measured in a cell proliferation assay using TF1 human erythroleukemic cells.
• Assay: Cell line: TF1 (human erythroleukemic cell); Maintenance Condition: 10% FBS RPMI 1640 with GM-CSF; Assay medium: 10% FBS RPMI 1640 without Gm-CSF; Cell density: 2 x 10,000 cells/well (96 well plate, final volume 100ul); Starvation: 24hr, Assay medium; Incubation time: 24 hr (after sample treatment); Concentration range: 0.048 ng/ml - 25 ng/ml; Detection method: MTT assay
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for GM-CSF active protein

Human GM-CSF is a 14.6 kDa globular protein consisting of 128 amino acid containing two dislufied bonds. GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in by endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. Recombinant human GM-CSF was expressed in E Coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Product Categories/Family for GM-CSF active protein

Hormones & Cytokines

References

Trapnell BC, et al. (2002) Annu Rev Physiol. 64, 775-80; Wong GG, et al (1985) Science, 228, 810-815;

NCBI and Uniprot Product Information

• NCBI GI #: 27437030
• NCBI GeneID: 1437
• NCBI Accession #: NP_000749
• NCBI GenBank Nucleotide #: NM_000758.3
• UniProt Accession #: P04141; Q14CE8; Q2VPI8; Q8NFI6
• Molecular Weight: 14.6 kDa (128 aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: granulocyte-macrophage colony-stimulating factor
• NCBI Official Synonym Full Names: colony stimulating factor 2
• NCBI Official Symbol: CSF2
• NCBI Official Synonym Symbols: CSF; GMCSF
• NCBI Protein Information: granulocyte-macrophage colony-stimulating factor
• UniProt Protein Name: Granulocyte-macrophage colony-stimulating factor
• UniProt Gene Name: CSF2
• UniProt Synonym Gene Names: GMCSF; GM-CSF; CSF

NCBI Description

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]

Uniprot Description

Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.