Thrombopoietin Active Protein | THPO active protein

Recombinant human Thrombopoietin protein

Cat. Num. AAA201283

• Gene Names: THPO; ML; TPO; MGDF; MKCSF; MPLLG; THCYT1
• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: Thrombopoietin; Recombinant human Thrombopoietin protein; THPO; MGDF; MKCSF; ML; MPLLG; THCYT1; TPO; thrombopoietin isoform 1 precursor; C-mpl ligand; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; Myeloproliferative leukemia virus oncogene ligand; ATGP3057; THPO active protein

• Host: HEK293
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 22-353aa
• Sequence: SPAPP ACDLRVLSKL LRDSHVLHSR LSQCPEVHPL PTPVLLPAVD FSLGEWKTQM EETKAQDILG AVTLLLEGVM AARGQLGPTC LSSLLGQLSG QVRLLLGALQ SLLGTQLPPQ GRTTAHKDPN AIFLSFQHLL RGKVRFLMLV GGSTLCVRRA PPTTAVPSRT SLVLTLNELP NRTSGLLETN FTASARTTGS GLLKWQQGFR AKIPGLLNQT SRSLDQIPGY LNRIHELLNG TRGLFPGPSR RTLGAPDISS GTSDTGSLPP NLQPGYSPSP THPPTGQYTL FPLPPTLPTP VVQLHPLLPD PSAPTPTPTS PLLNTSYTHS QNLSQEG

• Applicable Applications for THPO active protein: SDS-PAGE, Bioactivity
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Human
• Tag: His-Tag
• Predicted N-term: Asp
• Bioactivity: Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 range <=10ng/ml.
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Biological Activity

(Human Thrombopoietin stimulates cell proliferation of the MO7e human megakaryocytic leukemic cells. The ED50 range )

Related Product Information for THPO active protein

Thrombopoietin, also known as megakaryocyte growth and development factor (MGDF), is a member of EPO/TPO family. It is produced in the liver by both parenchymal cells and sinusoidal endothelial cells, as well as in the kidney by proximal convoluted tubule cells. It acts as a key regulator of megakaryocytopoiesis and thrombopoiesis that leads to platelet production. Also, this protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Thrombopoietin, like EPO, plays a role in brain development. It promotes apoptosis of newly generated neurons, an effect counteracted by EPO and neurotrophins. Recombinant human Thrombopoietin fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Categories/Family for THPO active protein

Cardiovascular; Proteins

References

Fishley B, et al, (2004) Growth Factors. 22:151-155. Chou FS, et al, (2011) J Cell Biochem. 112:1491-1498. Kong Z, et al, (2017) Blood. 130:1097-1103.

NCBI and Uniprot Product Information

• NCBI GI #: 4507493
• NCBI GeneID: 7066
• NCBI Accession #: NP_000451.1
• NCBI GenBank Nucleotide #: NM_000460.3
• UniProt Accession #: P40225; Q13020; Q15790; Q15791; Q15792; A1L3Y0; B7ZLR8; B9EGA8
• Molecular Weight: 33,604 Da
• NCBI Official Full Name: thrombopoietin isoform 1
• NCBI Official Synonym Full Names: thrombopoietin
• NCBI Official Symbol: THPO
• NCBI Official Synonym Symbols: ML; TPO; MGDF; MKCSF; MPLLG; THCYT1
• NCBI Protein Information: thrombopoietin
• UniProt Protein Name: Thrombopoietin
• Protein Family: Thrombopoietin
• UniProt Gene Name: THPO
• UniProt Synonym Gene Names: MGDF; ML; MGDF
• UniProt Entry Name: TPO_HUMAN

Uniprot Description

THPO: Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. Defects in THPO are the cause of thrombocythemia type 1 (THCYT1). A myeloproliferative disorder characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications. Belongs to the EPO/TPO family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Secreted; Cytokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: extracellular space

Molecular Function: growth factor activity; hormone activity; cytokine activity

Biological Process: positive regulation of protein kinase B signaling cascade; cell proliferation; platelet activation; multicellular organismal development; myeloid cell differentiation; positive regulation of megakaryocyte differentiation; positive regulation of protein amino acid phosphorylation; blood coagulation

Disease: Thrombocythemia 1