SDS-Page
GM-CSF active protein
GM-CSF, 18-141aa, Mouse, E Coli (Bioactivity Validated)
Gene Names
Csf2; CSF; Csfgm; GMCSF; Gm-CSf; MGI-IGM
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
GM-CSF; 18-141aa; Mouse; E Coli (Bioactivity Validated); Granulocyte-macrophage colony stimulating factor 2; Csf2; Csfgm; MGI-IGM; mGM-CSF; GM-CSF active protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid In phosphate-buffered saline (PBS), pH 7.4, 10% glycerol
Concentration
1 mg/ml (determined by BCA assay) (varies by lot)
Sequence
MAPTRSPITV TRPWKHVEAI KEALNLLDDM PVTLNEEVEV VSNEFSFKKL TCVQTRLKIF EQGLRGNFTK LKGALNMTAS YYQTYCPPTP ETDCETQVTT YADFIDSLKT FLTDIPFECK KPVQK
Sequence Length
141
Applicable Applications for GM-CSF active protein
SDS-PAGE
Antigen Species
Mouse
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Biological Activity
Measured in a cell proliferation assay using MC/9 cells. The ED50 for this effect is less or equal to 0.5 ng/ml.
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for GM-CSF active protein
GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in by endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. Mouse GM-CSF protein is 125 amino acids and shares approximately 60% homology with the human GM-CSF protein. Recombinant mouse GM-CSF was expressed in E Coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer
Product Categories/Family for GM-CSF active protein
References
Fleetwood AJ., et al, (2005). Crit Rev Immunol. 25 (5):405-28.; Hamilton JA., et al. (2004). Growth Factors. 22 (4):225-31.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
14 kDa (125 aa), confirmed by MALDI-TOF.
NCBI Official Full Name
granulocyte-macrophage colony-stimulating factor
NCBI Official Synonym Full Names
colony stimulating factor 2 (granulocyte-macrophage)
NCBI Official Symbol
Csf2
NCBI Official Synonym Symbols
CSF; Csfgm; GMCSF; Gm-CSf; MGI-IGM
NCBI Protein Information
granulocyte-macrophage colony-stimulating factor
UniProt Protein Name
Granulocyte-macrophage colony-stimulating factor
UniProt Gene Name
Csf2
UniProt Synonym Gene Names
Csfgm; GM-CSF; CSF
Uniprot Description
Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
Research Articles on GM-CSF
Similar Products
Product Notes
The GM-CSF csf2 (Catalog #AAA203121) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GM-CSF can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the GM-CSF csf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MAPTRSPITV TRPWKHVEAI KEALNLLDDM PVTLNEEVEV VSNEFSFKKL TCVQTRLKIF EQGLRGNFTK LKGALNMTAS YYQTYCPPTP ETDCETQVTT YADFIDSLKT FLTDIPFECK KPVQK. It is sometimes possible for the material contained within the vial of "GM-CSF, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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