IL-9 active protein

Recombinant IL-9

Cat. Num. AAA201177

• Gene Names: IL9; P40; HP40; IL-9
• Applications: SDS-Page
• Purity: > 85% by SDS - PAGE
• Synonyms: IL-9; Recombinant IL-9; Interleukin-9; HP40; IL9; P40; Cytokine P40; T-cell growth factor P40; IL-9 active protein

• Host: Baculovirus
• Purity/Purification: > 85% by SDS - PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
• Concentration: 0.5mg/ml (determined by BCA assay) (varies by lot)
• Sequence Positions: 19-144aa
• Sequence: QGCPTLAGIL DINFLINKMQ EDPASKCHCS ANVTSCLCLG IPSDNCTRPC FSERLSQMTN TTMQTRYPLI FSRVKKSVEV LKNNKCPYFS CEQPCNQTTA GNALTFLKSL LEIFQKEKMR GMRGKI
• Sequence Length: 144

• Applicable Applications for IL-9 active protein: SDS-PAGE; Bioactivity
• Tag: His-Tag
• Biological Activity: Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 range <= 0.3 ng/ml.
• Predicted nterm: Gln 19
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
• Preparation and Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoidrepeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )

Activity

(Human IL-9 stimulates cell proliferation of the MO7e human megakaryocytic leukemic cells. The ED50 range )

Related Product Information for IL-9 active protein

IL-9, also known as interleukin 9, is a cytokine (cell signalling molecule) belonging to the group ofinterleukins. This protein produced by T-cells and specifically by CD4+ helper cells that acts as a regulatorof a variety of hematopoietic cells and stimulates cell proliferation and prevents apoptosis. It functions throughthe interleukin-9 receptor (IL9R), which activates different signal transducer and activator (STAT)proteins and thus connects this cytokine to various biological processes. Recombinant human IL-9, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for IL-9 active protein

Hormones & Cytokines; Immunology

References

Purwar R, Schlapbach C, et al. (2012). Nat Med. 18(8):1248-53. ;;Pilette C, Ouadrhiri Y, et al. (2002). J Immunol. 168(8):4103-11.

NCBI and Uniprot Product Information

• NCBI GI #: 10834980
• NCBI GeneID: 3578
• NCBI Accession #: NP_000581.1
• NCBI GenBank Nucleotide #: NP_000581.1
• UniProt Accession #: P15248
• Molecular Weight: 14.9kDa (132aa);;Multiple bands between 18-28kDa, reducing conditions
• NCBI Official Full Name: interleukin-9
• NCBI Official Synonym Full Names: interleukin 9
• NCBI Official Symbol: IL9
• NCBI Official Synonym Symbols: P40; HP40; IL-9
• NCBI Protein Information: interleukin-9
• UniProt Protein Name: Interleukin-9
• UniProt Gene Name: IL9
• UniProt Synonym Gene Names: IL-9
• UniProt Entry Name: IL9_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness. [provided by RefSeq, Jul 2008]

Uniprot Description

IL9: Supports IL-2 independent and IL-4 independent growth of helper T-cells. Belongs to the IL-7/IL-9 family.

Protein type: Secreted; Secreted, signal peptide; Cytokine

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space

Molecular Function: growth factor activity; interleukin-9 receptor binding; cytokine activity

Biological Process: positive regulation of cell proliferation; immune response; inflammatory response; positive regulation of cell growth; positive regulation of interleukin-5 biosynthetic process