Rabbit anti-Human, Mouse BNIP3 Polyclonal Antibody | anti-BNIP3 antibody

Rabbit Anti-Human BNIP3 Polyclonal

Cat. Num. AAA807475

• Gene Names: BNIP3; NIP3
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Peptide Affinity Purified
• Synonyms: BNIP3; Polyclonal Antibody; Rabbit Anti-Human BNIP3 Polyclonal; NIP3 Antibody; BCL2 Adenovirus E1B 19kDa Interacting Protein 3 Antibody; BNIP3_Human Antibody; BNIP3 Antibody: Alkaline Phosphatase; BNIP3 Antibody; anti-BNIP3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Purity/Purification: Peptide Affinity Purified
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 259

• Applicable Applications for anti-BNIP3 antibody: Western Blot (WB)
• Application Notes: Western Blot: 1:1000
• Immunogen: Synthetic peptide from the C-terminal of human BNIP3
• Immunogen Species: Human
• COA: A 1:1000 dilution was sufficient for detection of BNIP3 on mouse kidney lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
• Storage Buffer: PBS, 50% glycerol, 0.09% sodium azide
• Conjugate: Alkaline Phosphatase
• Cellular Localization: Mitochondrion, Mitochondrion outer membrane
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of Human HeLa and HEK293T cell lysates showing detection of ~30kDa BNIP3 protein using Rabbit Anti-BNIP3 Polyclonal Antibody (SPC-685). Lane 1: MW Ladder. Lane 2: Human HeLa (20 ug). Lane 3: Human 293T (20 ug). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BNIP3 Polyclonal Antibody (SPC-685) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~30kDa .)

Western Blot (WB)

(Western blot analysis of Mouse Kidney showing detection of ~30kDa BNIP3 protein using Rabbit Anti-BNIP3 Polyclonal Antibody (SPC-685). Lane 1: MW Ladder. Lane 2: Mouse Kidney (20 ug). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BNIP3 Polyclonal Antibody (SPC-685) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~30kDa . Other Band(s): ~60kDa (BNIP3 complex).)

Related Product Information for anti-BNIP3 antibody

BNIP3 is an apoptosis-inducing protein which can overcome BCL2 suppression. It may play a role in repartioning calcium between the two major intracellular calcium stores in association with BCL2. It has been shown to interact with CD47, BCL2-like, and BCL-2 (1-3).

Product Categories/Family for anti-BNIP3 antibody

Cancer; Apoptosis; Cardiovascular System; Heart

References

1. Boyd J.M., et al. (1994) Cell. 79(2): 341-351. 2. Lamy L., et al. (2003) J Biol Chem. 278(26): 23915-23921. 3. Ray R., et al. (2000) J Biol Chem. 275(2): 1439-1448.

NCBI and Uniprot Product Information

• NCBI GI #: 734703979
• NCBI GeneID: 664
• NCBI Accession #: NP_004043.3
• NCBI GenBank Nucleotide #: NM_004052.3
• UniProt Accession #: Q12983; O14620; Q96GP0
• Molecular Weight: Predicted molecular weight at ~21.5kDa. Observed bands at ~20-30kDa and 50-60kDa due to the homodimer complex of BNIP3.
• NCBI Official Full Name: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
• NCBI Official Synonym Full Names: BCL2/adenovirus E1B 19kDa interacting protein 3
• NCBI Official Symbol: BNIP3
• NCBI Official Synonym Symbols: NIP3
• NCBI Protein Information: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
• UniProt Protein Name: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
• Protein Family: BNIP3
• UniProt Gene Name: BNIP3
• UniProt Synonym Gene Names: NIP3
• UniProt Entry Name: BNIP3_HUMAN

NCBI Description

This gene is encodes a mitochondrial protein that contains a BH3 domain and acts as a pro-apoptotic factor. The encoded protein interacts with anti-apoptotic proteins, including the E1B 19 kDa protein and Bcl2. This gene is silenced in tumors by DNA methylation. [provided by RefSeq, Dec 2014]

Uniprot Description

BNIP3: Apoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Homodimer. Binds to BCL2. Interacts with BNIP3L and ACAA2. Also can interact with adenovirus E1B 19 kDa protein or Epstein-Barr virus BHRF1. Belongs to the NIP3 family.

Protein type: Mitochondrial; Membrane protein, integral

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: cytoplasm; dendrite; endoplasmic reticulum; integral to mitochondrial outer membrane; mitochondrial membrane; mitochondrial outer membrane; mitochondrion; nuclear envelope; nucleoplasm; nucleus

Molecular Function: GTPase binding; identical protein binding; protein binding; protein heterodimerization activity; protein homodimerization activity

Biological Process: apoptosis; autophagic cell death; brown fat cell differentiation; cell death; defense response to virus; induction of apoptosis by granzyme; mitochondrial fragmentation during apoptosis; mitochondrion degradation; negative regulation of apoptosis; negative regulation of membrane potential; neuron apoptosis; positive regulation of apoptosis; positive regulation of autophagy; positive regulation of macroautophagy; positive regulation of programmed cell death; positive regulation of protein complex disassembly; regulation of mitochondrial membrane permeability; response to hyperoxia; response to hypoxia; viral reproduction