Rat BMP-7 PicoKine ELISA Kit | Bmp7 elisa kit

Rat BMP-7 PicoKine ELISA Kit

Cat. Num. AAA177567

• Gene Names: Bmp7; BMP-7
• Reactivity: Rat
• Synonyms: BMP-7 PicoKine; Rat BMP-7 PicoKine ELISA Kit; Bone morphogenetic protein 7; BMP 7; BMP7; BMP-7; Eptotermin alfa; OP 1; OP1; OP1-; Osteogenic protein 1; bone morphogenetic protein 7; Bmp7 elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant rat BMP-7
• Sequence Length: 306

• Assay Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <10pg/ml
• Sample: For quantitative detection of rat BMP-7 in bone tissue, cell culture supernates, serum and plasma((heparin, EDTA).
• Immunogen: CHO, S292-H430
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml rat BMP-7 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat bone tissue, cell culture supernates, serum or plasma((heparin, EDTA) to each empty well. It is recommended that each rat BMP-7 standard solution and each sample be measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml)|135|609|1288|198|716|1563; Standard deviation|6.08|32.28|66.98|15.05|58.71|109.41; CV(%)|4.5|5.3|5.2|7.6|8.2|7.0
• Sample Data: Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000; O.D.|0.084|0.129|0.171|0.233|0.391|0.712|1.320|2.240
• Anticoagulant: Heparin or EDTA
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Rat BMP-7 PicoKine ELISA Kit standard curve )

Related Product Information for Bmp7 elisa kit

Principle of the assay: Rat BMP-7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BMP-7 has been precoated onto 96-well plates. Standards(Expression system for standard: CHO, Immunogen sequence: S292-H430) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat BMP-7 amount of sample captured in plate.

Background: Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene.The protein encoded by this gene is a member of the TGF-beta superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.

NCBI and Uniprot Product Information

• NCBI GI #: 81907235
• NCBI GeneID: 85272
• UniProt Accession #: Q9R228
• Molecular Weight: 34,953 Da
• NCBI Official Full Name: Bone morphogenetic protein 7
• NCBI Official Synonym Full Names: bone morphogenetic protein 7
• NCBI Official Symbol: Bmp7
• NCBI Official Synonym Symbols: BMP-7
• NCBI Protein Information: bone morphogenetic protein 7
• UniProt Protein Name: Bone morphogenetic protein 7
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: Bmp7
• UniProt Entry Name: Q9R228_RAT

NCBI Description

binds to cell surface heparan sulfate [RGD, Feb 2006]