Mouse BMP-7 PicoKine ELISA Kit | Bmp7 elisa kit

Mouse BMP-7 PicoKine ELISA Kit

Cat. Num. AAA177606

• Gene Names: Bmp7; OP1
• Reactivity: Mouse
• Synonyms: BMP-7 PicoKine; Mouse BMP-7 PicoKine ELISA Kit; Bone morphogenetic protein 7; BMP 7; BMP7; BMP-7; Eptotermin alfa; OP 1; OP1; OP1-; Osteogenic protein 1; bone morphogenetic protein 7; Bmp7 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse BMP-7
• Sequence Length: 430

• Assay Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <10pg/ml
• Sample: For quantitative detection of mouse BMP-7 in bone tissue, cell culture supernates, serum and plasma((heparin, EDTA).
• Immunogen: CHO, S292-H430
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml mouse BMP-7 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse bone tissue, cell culture supernates, serum or plasma((heparin, EDTA) to each empty well. It is recommended that each mouse BMP-7 standard solution and each sample be measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml)|185|665|1408|210|887|1699; Standard deviation|9.25|43.89|66.18|14.91|70.96|132.52; CV(%)|5.0|6.6|4.7|7.1|8.0|7.8
• Sample Data: Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000; O.D.|0.032|0.085|0.143|0.248|0.423|0.816|1.359|2.231
• Anticoagulant: Heparin or EDTA
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Mouse BMP-7 PicoKine ELISA Kit standard curve )

Related Product Information for Bmp7 elisa kit

Principle of the assay: Mouse BMP-7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for BMP-7 has been precoated onto 96-well plates. Standards(Expression system for standard: CHO, Immunogen sequence: S292-H430) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse BMP-7 amount of sample captured in plate.

Background: Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene.The protein encoded by this gene is a member of the TGF-beta superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.

NCBI and Uniprot Product Information

• NCBI GI #: 31982487
• NCBI GeneID: 12162
• NCBI Accession #: NP_031583.2
• NCBI GenBank Nucleotide #: NM_007557.3
• UniProt Accession #: P23359; Q91XF7
• Molecular Weight: 49,199 Da
• NCBI Official Full Name: bone morphogenetic protein 7 preproprotein
• NCBI Official Synonym Full Names: bone morphogenetic protein 7
• NCBI Official Symbol: Bmp7
• NCBI Official Synonym Symbols: OP1
• NCBI Protein Information: bone morphogenetic protein 7
• UniProt Protein Name: Bone morphogenetic protein 7
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: Bmp7
• UniProt Synonym Gene Names: Bmp-7; Op1; BMP-7; OP-1
• UniProt Entry Name: BMP7_MOUSE

NCBI Description

This gene encodes a member of a family of secreted factors that participate in developmental signal transduction to stimulate SMAD transcriptional activators. Mutation of this gene results in skeletal, kidney, and other developmental defects. The propeptide is further cleaved into an active chain. [provided by RefSeq, Mar 2013]

Uniprot Description

BMP7: Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis. Homodimer; disulfide-linked. Interacts with SOSTDC1. Interacts with TWSG1. Expressed in the kidney and bladder. Lower levels seen in the brain. Belongs to the TGF-beta family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular matrix; extracellular region; extracellular space

Molecular Function: cytokine activity; growth factor activity; heparin binding; protein binding; transforming growth factor beta receptor binding

Biological Process: anatomical structure formation; axon guidance; BMP signaling pathway; branching morphogenesis of a tube; camera-type eye morphogenesis; cartilage development; cell development; cell differentiation; embryonic camera-type eye morphogenesis; embryonic limb morphogenesis; embryonic pattern specification; epithelial cell differentiation; eye development; growth; kidney development; mesenchymal cell differentiation; mesoderm formation; multicellular organismal development; negative regulation of cell cycle; negative regulation of cell proliferation; negative regulation of MAP kinase activity; negative regulation of mitosis; negative regulation of neurogenesis; negative regulation of neuron differentiation; negative regulation of Notch signaling pathway; negative regulation of phosphorylation; negative regulation of transcription, DNA-dependent; neurite morphogenesis; odontogenesis of dentine-containing teeth; organ morphogenesis; ossification; pattern specification process; positive regulation of apoptosis; positive regulation of bone mineralization; positive regulation of cell differentiation; positive regulation of heterotypic cell-cell adhesion; positive regulation of neuron differentiation; positive regulation of osteoblast differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of MAPKKK cascade; regulation of phosphorylation; salivary gland morphogenesis; steroid hormone mediated signaling; tube morphogenesis; ureteric bud development