Typical Testing Data/Standard Curve (for reference only)
(Mouse CXCL9 PicoKine ELISA Kit standard curve )
Mouse CXCL9 PicoKine ELISA Kit | Cxcl9 elisa kit
Mouse CXCL9 PicoKine ELISA Kit
Gene Names
Cxcl9; CMK; Mig; MuMIG; Scyb9; crg-10; BB139920
Reactivity
Mouse
Synonyms
CXCL9 PicoKine; Mouse CXCL9 PicoKine ELISA Kit; C-X-C motif chemokine 9; Chemokine (C-X-C motif) ligand 9; CMK; Crg 10; CXCL9; CXCL9_HUMAN; Gamma interferon induced monokine; Gamma-interferon-induced monokine; HuMIG; MIG; Monokine induced by gamma interferon; Monokine induced by interferon gamma; Monokine induced by interferon-gamma; SCYB9; Small inducible cytokine B9; Small inducible cytokine subfamily B member 9; Small-inducible cytokine B9; chemokine (C-X-C motif) ligand 9; Cxcl9 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse CXCL9
Sequence Length
126
Assay Range
31.2pg/ml-2000pg/ml
Sensitivity
<10pg/ml
Sample
For quantitative detection of mouse CXCL9 in cell culture supernates, serum and plasma(EDTA).
Immunogen
E Coli, T22-T126
Assay Procedure Step 1
Aliquot 0.1ml per well of the 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml mouse CXCL9 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(EDTA) to each empty well. It is recommended that each mouse CXCL9 standard solution and each sample be measured in duplicate.
Intra-Assay Precision
Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|113|468|1221|126|471|1288
Standard deviation|4.86|22.00|69.60|10.46|41.92|117.21
CV(%)|4.3|4.7|5.7|8.3|8.9|9.1
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|113|468|1221|126|471|1288
Standard deviation|4.86|22.00|69.60|10.46|41.92|117.21
CV(%)|4.3|4.7|5.7|8.3|8.9|9.1
Sample Data
Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000
O.D.|0.031|0.074|0.157|0.305|0.587|1.032|1.901|2.369
O.D.|0.031|0.074|0.157|0.305|0.587|1.032|1.901|2.369
Anticoagulant
EDTA
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
(Mouse CXCL9 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Mouse CXCL9 PicoKine ELISA Kit standard curve )
Related Product Information for Cxcl9 elisa kit
Principle of the assay: Mouse CXCL9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CXCL9 has been precoated onto 96-well plates. Standards(E Coli, T22-T126) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CXCL9 amount of sample captured in plate.
Background: Chemokine (C-X-C motif) ligand 9 (CXCL9) is a small cytokine belonging to the CXC chemokine family that is also known as Monokine induced by gamma interferon (MIG). CXCL9 is a T-cell chemoattractant, which is induced by IFN-gamma. It is closely related to two other CXC chemokines called CXCL10 and CXCL11, whose genes are located near the gene for CXCL9 on human chromosome 4. CXCL9, CXCL10 and CXCL11 all elicit their chemotactic functions by interacting with the chemokine receptor CXCR3. The standard product used in this kit is recombinant human CXCL9, consisting of 103 amino acids with the molecular mass of 11.7KDa.
Background: Chemokine (C-X-C motif) ligand 9 (CXCL9) is a small cytokine belonging to the CXC chemokine family that is also known as Monokine induced by gamma interferon (MIG). CXCL9 is a T-cell chemoattractant, which is induced by IFN-gamma. It is closely related to two other CXC chemokines called CXCL10 and CXCL11, whose genes are located near the gene for CXCL9 on human chromosome 4. CXCL9, CXCL10 and CXCL11 all elicit their chemotactic functions by interacting with the chemokine receptor CXCR3. The standard product used in this kit is recombinant human CXCL9, consisting of 103 amino acids with the molecular mass of 11.7KDa.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14,444 Da
NCBI Official Full Name
C-X-C motif chemokine 9
NCBI Official Synonym Full Names
chemokine (C-X-C motif) ligand 9
NCBI Official Symbol
Cxcl9
NCBI Official Synonym Symbols
CMK; Mig; MuMIG; Scyb9; crg-10; BB139920
NCBI Protein Information
C-X-C motif chemokine 9
UniProt Protein Name
C-X-C motif chemokine 9
Protein Family
UniProt Gene Name
Cxcl9
UniProt Synonym Gene Names
Mig; Scyb9; MIG; MuMIG
UniProt Entry Name
CXCL9_MOUSE
Uniprot Description
CXCL9: Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3. Belongs to the intercrine alpha (chemokine CxC) family.
Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis
Cellular Component: external side of plasma membrane; extracellular region; extracellular space
Molecular Function: chemokine activity; CXCR chemokine receptor binding; cytokine activity
Biological Process: chemotaxis; defense response to virus; G-protein coupled receptor protein signaling pathway; immune response; inflammatory response; positive regulation of leukocyte chemotaxis; positive regulation of myoblast differentiation; regulation of cell proliferation; response to lipopolysaccharide
Research Articles on Cxcl9
Similar Products
Product Notes
The Mouse Cxcl9 cxcl9 (Catalog #AAA177601) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177601 ELISA Kit recognizes Mouse Cxcl9. It is sometimes possible for the material contained within the vial of "CXCL9 PicoKine, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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