Mouse HGF PicoKine ELISA Kit | Hgf elisa kit

Mouse HGF PicoKine ELISA Kit

Cat. Num. AAA177605

• Gene Names: Hgf; SF; NK1; NK2; HGF/SF; SF/HGF; C230052L06Rik
• Reactivity: Mouse
• Synonyms: HGF PicoKine; Mouse HGF PicoKine ELISA Kit; Hepatocyte growth factor; DFNB39; F TCF; Fibroblast derived tumor cytotoxic factor; Hepapoietin A; Hepatocyte growth factor (hepapoietin A scatter factor; Hepatocyte growth factor beta chain; Hepatopoietin-A; Hgf; HGF_HUMAN; HGFB; HPTA; Lung fibroblast derived mitogen; Scatter factor; SF; hepatocyte growth factor (hepapoietin A; scatter factor); Hgf elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse HGF
• Sequence Length: 728

• Assay Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <10pg/ml
• Sample: For quantitative detection of mouse HGF in cell culture supernates, serum and plasma(EDTA, citrate).
• Immunogen: NSO,Q33-R495 (alpha)&V496-L728 (beta)
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml mouse HGF standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(EDTA, citrate) to each empty well. We recommend that each mouse HGF standard solution and each sample is measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml)|427|1523|3342|501|1756|3471; Standard deviation|17.93|76.15|187.15|37.58|136.97|284.62; CV(%)|4.2|5.0|5.6|7.5|7.8|8.2
• Sample Data: Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000; O.D.|0.055|0.091|0.132|0.226|0.412|0.730|1.331|2.160
• Anticoagulant: Heparin or EDTA
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Mouse HGF PicoKine ELISA Kit standard curve )

Related Product Information for Hgf elisa kit

Principle of the assay: Mouse HGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for HGF has been precoated onto 96-well plates. Standards(NSO,Q33-R495 (alpha) & V496 - L728 (beta)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for HGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse HGF amount of sample captured in plate.

Background: Hepatocyte growth factor(HGF) is the most potent mitogen for mature parenchymal hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. HGF has a relative molecular mass(Mr) of 82,000 and is a heterodimer composed of a large alpha-subunit of Mr 69,000 and a small beta-subunit of Mr 34,000. The protein consists of 728 amino acid residues, including a possible signal peptide at the N-terminus. HGF may serve as a paracrine mediator to control placental development and growth. This growth factor may play an important role as a paracrine mediator of the proliferation of melanocytes and endothelial cells, as well as cells of epithelial origin. The gene encoding the human HGF is assigned to human chromosome 7.

NCBI and Uniprot Product Information

• NCBI GI #: 575501559
• NCBI GeneID: 15234
• NCBI Accession #: NP_001276387.1
• NCBI GenBank Nucleotide #: NM_001289458.1
• UniProt Accession #: Q08048; O55027; Q53WS5; Q61662; Q64007; Q6LBE6
• Molecular Weight: 24,308 Da
• NCBI Official Full Name: hepatocyte growth factor isoform 1 preproprotein
• NCBI Official Synonym Full Names: hepatocyte growth factor
• NCBI Official Symbol: Hgf
• NCBI Official Synonym Symbols: SF; NK1; NK2; HGF/SF; SF/HGF; C230052L06Rik
• NCBI Protein Information: hepatocyte growth factor
• UniProt Protein Name: Hepatocyte growth factor
• Protein Family: Hepatocyte growth factor
• UniProt Gene Name: Hgf
• UniProt Synonym Gene Names: SF
• UniProt Entry Name: HGF_MOUSE

NCBI Description

This gene encodes a protein that binds to the hepatocyte growth factor receptor to regulate cell growth, cell motility and morphogenesis in numerous cell and tissue types. The encoded preproprotein is proteolytically processed to generate multiple protein products, including the hepatocyte growth factor alpha and beta chains, which heterodimerize to form the mature active protein. Although this protein is a member of the peptidase S1 family of serine proteases, it lacks peptidase activity. Homozygous knockout mice for this gene exhibit embryonic lethality due to impaired development of the placenta and liver. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]

Uniprot Description

HGF: HGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as growth factor for a broad spectrum of tissues and cell types. It has no detectable protease activity. Activating ligand for the receptor tyrosine kinase MET by binding and promoting its dimerization. Defects in HGF are the cause of deafness autosomal recessive type 39 (DFNB39). A form of profound prelingual sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Belongs to the peptidase S1 family. Plasminogen subfamily. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Hormone; Cell development/differentiation; Motility/polarity/chemotaxis

Cellular Component: extracellular space; membrane

Molecular Function: chemoattractant activity; growth factor activity; identical protein binding; protein heterodimerization activity; serine-type endopeptidase activity

Biological Process: activation of MAPK activity; cell morphogenesis; cell proliferation; hepatocyte growth factor receptor signaling pathway; liver development; myoblast proliferation; negative regulation of apoptosis; negative regulation of caspase activity; negative regulation of inflammatory response; negative regulation of interleukin-6 production; negative regulation of peptidyl-serine phosphorylation; positive chemotaxis; positive regulation of angiogenesis; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of interleukin-10 production; positive regulation of myelination; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; proteolysis