Rat CXCL2/MIP-2 PicoKine ELISA Kit | Cxcl2 elisa kit

Rat CXCL2/MIP-2 PicoKine ELISA Kit

Cat. Num. AAA177622

• Gene Names: Cxcl2; Mip-2; Scyb2
• Reactivity: Rat
• Synonyms: CXCL2/MIP-2 PicoKine; Rat CXCL2/MIP-2 PicoKine ELISA Kit; C-X-C motif chemokine 2; Chemokine (C-X-C motif) ligand 2; CINC-2a; Cxcl2; CXCL2_HUMAN; Gro beta; Gro-beta; GRO-beta(5-73); GRO-beta-T; GRO2; GRO2 oncogene; GROb; Growth-regulated protein beta; Hematopoietic synergistic factor; HSF; Macrophage inflammatory protein 2-alpha; Melanoma growth stimulatory activity beta; MGSA beta; MGSA-b; MIP 2; MIP 2a; MIP2; MIP2-alpha; MIP2A; SB-251353; SCYB2; chemokine (C-X-C motif) ligand 2; Cxcl2 elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant rat MIP-2
• Sequence Length: 100

• Assay Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <10pg/ml
• Sample: For quantitative detection of rat MIP-2 in cell culture supernates, serum and plasma(heparin, EDTA).
• Immunogen: E Coli, S32-N100
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml rat MIP-2 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each rat MIP-2 standard solution and each sample be measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml)|113|355|627|134|387|653; Standard deviation|4.97|18.46|25.73|6.96|21.28|39.18; CV(%)|4.4|5.2|5.7|5.2|5.5|6.0
• Sample Data: Concentration(pg/ml)|0|15.6|31.2|62.5|125|250|500|1000; O.D.|0.059|0.124|0.156|0.244|0.451|0.684|1.346|1.916
• Anticoagulant: Heparin or EDTA
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Rat CXCL2 PicoKine ELISA Kit standard curve )

Related Product Information for Cxcl2 elisa kit

Principle of the assay: Rat MIP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MIP-2 has been precoated onto 96-well plates. Standards (E Coli, S32-N100) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MIP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat MIP-2 amount of sample captured in plate.

Background: MIP is a member of the aquaporin family of membrane-bound water channels. MIP family proteins are thought to contain 6 TM domains. Sequence analysis suggests that the proteins may have arisen through tandem, intragenic duplication from an ancestral protein that contained 3 TM domains. Major intrinsic protein (MIP, also called MP26) is the predominant fiber cell membrane protein of the ocular lens. The major intrinsic protein (MIP) of the vertebrate eye lens is the first identified member of a sequence-related family of cell-membrane proteins that appears to have evolved by gene duplication. Several members of the MIP family transport water (aquaporins), glycerol and other small molecules in microbial, plant and animal cells.

NCBI and Uniprot Product Information

• NCBI GI #: 16758460
• NCBI GeneID: 114105
• NCBI Accession #: NP_446099.1
• NCBI GenBank Nucleotide #: NM_053647.1
• UniProt Accession #: P30348; Q5WA60
• Molecular Weight: 10,783 Da
• NCBI Official Full Name: C-X-C motif chemokine 2
• NCBI Official Synonym Full Names: C-X-C motif chemokine ligand 2
• NCBI Official Symbol: Cxcl2
• NCBI Official Synonym Symbols: Mip-2; Scyb2
• NCBI Protein Information: C-X-C motif chemokine 2
• UniProt Protein Name: C-X-C motif chemokine 2
• Protein Family: C-X-C motif chemokine
• UniProt Gene Name: Cxcl2
• UniProt Synonym Gene Names: Cinc3; Mip-2; Mip2; Scyb2; CINC-3; MIP2
• UniProt Entry Name: CXCL2_RAT

NCBI Description

chemokine involved in the pulmonary inflammatory response [RGD, Feb 2006]

Uniprot Description

CXCL3: Ligand for CXCR2. Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Cellular Component: cell; extracellular space

Molecular Function: chemokine activity; CXCR chemokine receptor binding

Biological Process: elevation of cytosolic calcium ion concentration; G-protein coupled receptor protein signaling pathway; immune response; inflammatory response; leukocyte chemotaxis; neutrophil chemotaxis; regulation of cell proliferation; response to amphetamine; response to estradiol stimulus; response to gamma radiation; response to glucocorticoid stimulus; response to heat; response to molecule of bacterial origin; wound healing