Mouse XCL1/Lymphotactin PicoKine ELISA Kit | Xcl1 elisa kit

Mouse XCL1/Lymphotactin PicoKine ELISA Kit

Cat. Num. AAA177584

• Gene Names: Xcl1; LTN; ATAC; Lptn; SCM-1; Scyc1; SCM-1a; AI661682
• Reactivity: Mouse
• Synonyms: XCL1/Lymphotactin PicoKine; Mouse XCL1/Lymphotactin PicoKine ELISA Kit; Lymphotactin; ATAC; C motif chemokine 1; Chemokine (C motif) ligand 1; Cytokine SCM-1; Cytokine SCM1; LPTN; LTN; Lymphotaxin; SCM 1; SCM 1 alpha; SCM-1-alpha; SCM1; SCM1 alpha; SCYC1; Small inducible cytokine C1; Small inducible cytokine subfamily C member 1; Small-inducible cytokine C1; XC chemokine ligand 1; Xcl1; XCL1_HUMAN; chemokine (C motif) ligand 1; Xcl1 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse XCL1
• Sequence Length: 114

• Assay Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <5pg/ml
• Sample: For quantitative detection of mouse XCL1 in cell culture supernates, serum and plasma(heparin, EDTA).
• Immunogen: E Coli, V22-G114
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml mouse XCL1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse XCL1 standard solution and each sample be measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml)|447|1253|2346|450|1238|2625; Standard deviation|28.61|53.88|115|39.15|64.38|144.4; CV(%)|6.4|4.3|4.9|8.7|5.2|5.5
• Sample Data: Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000; O.D.|0.004|0.036|0.071|0.147|0.321|0.733|1.673|2.409
• Anticoagulant: Heparin or EDTA
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Mouse XCL1/Lymphotactin PicoKine ELISA Kit standard curve )

Related Product Information for Xcl1 elisa kit

Principle of the assay: Mouse XCL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for XCL1 has been precoated onto 96-well plates. Standards (E Coli, V22-G114) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for XCL1is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse XCL1 amount of sample captured in plate.

Background: Chemokine(C motif) ligand(XCL1) is a small cytokine belonging to the XC chemokine family that is also known as lymphotactin. This gene is mapped to chromosome 1q23 by FISH. The sequence of the deduced 114-amino acid protein is most homologous to the CC chemokines CCL8 and CCL3. It is found in high levels in spleen, thymus, intestine and peripheral blood leukocytes, and at lower levels in lung, prostate gland and ovary. XCL1 induces the migration of cells expressing XCR1.

NCBI and Uniprot Product Information

• NCBI GI #: 6678712
• NCBI GeneID: 16963
• NCBI Accession #: NP_032536.1
• NCBI GenBank Nucleotide #: NM_008510.1
• UniProt Accession #: P47993
• Molecular Weight: 12,467 Da
• NCBI Official Full Name: lymphotactin
• NCBI Official Synonym Full Names: chemokine (C motif) ligand 1
• NCBI Official Symbol: Xcl1
• NCBI Official Synonym Symbols: LTN; ATAC; Lptn; SCM-1; Scyc1; SCM-1a; AI661682
• NCBI Protein Information: lymphotactin
• UniProt Protein Name: Lymphotactin
• Protein Family: Lymphotactin
• UniProt Gene Name: Xcl1
• UniProt Synonym Gene Names: Lptn; Ltn; Scyc1
• UniProt Entry Name: XCL1_MOUSE

Uniprot Description

XCL1: Chemotactic activity for lymphocytes but not for monocytes or neutrophils. Belongs to the intercrine gamma family.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Cellular Component: extracellular region; extracellular space

Molecular Function: CCR chemokine receptor binding; chemokine activity; chemokine receptor binding; cytokine activity; protein homodimerization activity

Biological Process: cell-cell signaling; chemotaxis; G-protein coupled receptor protein signaling pathway; immune response; inflammatory response; monocyte chemotaxis; negative regulation of interferon-gamma production; negative regulation of interleukin-2 production; negative regulation of T cell cytokine production; negative regulation of transcription factor activity; negative regulation of transcription, DNA-dependent; positive regulation of GTPase activity; positive regulation of immune response to tumor cell; positive regulation of inflammatory response; positive regulation of innate immune response; positive regulation of interleukin-10 production; positive regulation of leukocyte chemotaxis; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of T cell cytokine production; positive regulation of T cell mediated cytotoxicity; regulation of inflammatory response; release of sequestered calcium ion into cytosol; response to virus