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43 results for " Neuron Synapse Markers" - showing 1-43
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 7E3. Tissue: Neocortex. Species: Rat. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:1000.)
PSD95, Monoclonal Antibody (Cat# AAA804156)
Full Name
PSD95 Antibody
Gene Names
Dlg4; Dlgh4; PSD95; Sap90
Reactivity
Mouse, Rat, Bovine, Human
Applications
WB, IHC, ICC, IF, AM
Pricing
$405/0.1 mg | $1,585/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Early stages of filaggrin-like and dermal staining.)
SHANK3, Monoclonal Antibody (Cat# AAA802748)
Full Name
SHANK3 Antibody
Gene Names
Shank3; Prosap2
Reactivity
Mouse, Human, Rat
Applications
WB, IHC, ICC, IF, IP, AM
Pricing
$425/0.1 mg | $1,690/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 7E3 . Tissue: Neocortex. Species: Rat. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:1000.)
PSD95, Monoclonal Antibody (Cat# AAA8001880)
Full Name
PSD95 Antibody
Gene Names
Dlg4; Dlgh4; PSD95; Sap90
Reactivity
Human, Mouse, Rat, Bovine
Applications
WB, IHC, ICC, IF, AM
Purity
Protein G Purified
Pricing
$255/0.025 mg | $920/5x0.025 mg
FXR2, Monoclonal Antibody (Cat# AAA375028)
Full Name
Mouse Anti-FXR2 [A42]
Gene Names
FXR2; FXR2P; FMR1L2
Reactivity
Human. Predicted to work with: Mouse
Applications
EIA, ICC, IF, IP, WB
Purity
Protein A
Pricing
$470/0.1 mL | $1,960/5x0.1 mL
Testing Data
(Image: Mixed neuron-glial cultures stained with ACIII (red) and to aII-spectrin (green). Note the strong and clean staining of neuronal cilia. Since aII-spectrin is specific for neurons in the CNS, the glial cells in this culture are not recognized by this antibody. The aII-spectrin antibody is also an excellent marker of neuronal plasma membranes. Protocol on data-sheet.)
Adenylate Cyclase III/ADCY3, Antibody (Cat# AAA555189)
Full Name
Adenylate Cyclase III/ADCY3, Antiserum, Rabbit
Gene Names
ADCY3; AC3; AC-III
Reactivity
Cat, Chicken, Human, Mouse, Rat
Applications
ICC, IHC, WB
Pricing
$435/0.1 mL | $1,815/5x0.1 mL
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 6G6 . Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at-20 degree C. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Junction staining.)
PSD95, Monoclonal Antibody (Cat# AAA8001879)
Full Name
PSD95 Antibody
Gene Names
Dlg4; Dlgh4; PSD95; Sap90
Reactivity
Human, Mouse, Rat, Bovine
Applications
WB, IHC, ICC, IF, AM
Purity
Protein G Purified
Pricing
$255/0.025 mg | $920/5x0.025 mg
Testing Data
(Image: rat mixed neuron/glial cultures stained with 14-3-3 eta antibody (red). Neuronal perikarya are very rich in 14-3-3 eta which has a diffuse cytoplasmic staining pattern. Blue is a DNA stain. Protocol on data-sheet.)
14.3.3 (eta), Antibody (Cat# AAA555034)
Full Name
14.3.3 (eta), Monoclonal Antibody, Mouse
Gene Names
YWHAH; YWHA1
Reactivity
Human, Mouse, Rat
Applications
IF, WB
Pricing
$435/0.1 mL | $1,815/5x0.1 mL
Testing Data
(Image: Ki67staining of human tonsil tissue (paraffin embedded).)
Ki67, Antibody (Cat# AAA555143)
Full Name
Ki67, Affinity Purified Antibody, Rabbit
Reactivity
Human, Mouse, Porcine
Applications
ICC, IF, IHC
Pricing
$505/0.1 mL | $2,125/5x0.1 mL
Testing Data
NMDA splice variant N1, Antibody (Cat# AAA555146)
Full Name
NMDA splice variant N1, Affinity Purified Antibody, Rabbit
Gene Names
Nmdar1; CG2902; DmelNmdar1; DmelCG2902; DMNMDAR1; DNmdar; dnmdar-I; DNMDAR-I; dNR1; DrNR1; dsNR1; NMDA; NMDA R1; Nmdar; NMdar1; NMDAR1; nmr; NR1; NRI
Reactivity
Rat
Applications
IHC, WB
Pricing
$585/0.025 mg | $2,480/5x0.025 mg
Testing Data
(Image: Calretinin staining of human mesotheliom. Note cytoplasmic staining of malignant cells. Paraffin section.)
Calretinin (CAL6), Antibody (Cat# AAA555069)
Full Name
Calretinin (CAL6), Monoclonal Antibody, Mouse
Reactivity
Human
Applications
IHC
Pricing
$270/0.1 mL | $1,075/5x0.1 mL
Testing Data
(Image: Mixed cultures of rat CNS cells stained with alpha-Internexin (red) and chicken antibody to Microtubule associated protein 2 (MAP2- green). The a-internexin is localized primarily in neuronal axons in these cultures, while the perikarya and dendrites of neurons stain strongly for MAP2.)
alpha-Internexin [1D2], Antibody (Cat# AAA555019)
Full Name
Mouse Monoclonal Anti-alpha-Internexin [1D2]
Gene Names
INA; NEF5; NF-66; TXBP-1
Reactivity
Human, Rat
Applications
ICC, IF, WB
Pricing
$425/0.5 mL | $1,760/5x0.5 mL
Testing Data
(Image: A tissue section through an adult mouse brain showing GAD-67 (red staining) (cat # CH23031) in basket cells of the hippocampal formation. Photo courtesy of Dr. Felix Eckenstein, University of Vermont)
GAD67, Antibody (Cat# AAA555616)
Full Name
GAD67, Affinity Purified Antibody
Gene Names
GAD1; GAD; SCP; CPSQ1
Reactivity
Human, Mouse, Rat
Applications
ICC, IHC, WB
Pricing
$210/0.1 mL | $800/5x0.1 mL
Testing Data
(Image: Dissociated cell cultures of neonatal mouse brains, showing NSE (green staining) in neurons.These cultures were counter-stained with a rabbit anti-GFAP to localize astrocytes, as well as with DAPI (blue staining) to localize nuclei.)
NSE (Neuron-Specific Enolase), Antibody (Cat# AAA555388)
Full Name
NSE (Neuron-Specific Enolase), Affinity Purified Antibody, Chicken
Gene Names
ENO2; NSE
Reactivity
Human, Mouse
Applications
ICC, IHC, WB
Pricing
$210/0.1 mL | $800/5x0.1 mL
Testing Data
(Image: NMDAR2A Western Blot Analysis:)
NMDA Receptor 2A, Antibody (Cat# AAA555145)
Full Name
Anti-NMDA Receptor 2A, Affinity Purified Antibody
Reactivity
Human, Rat
Applications
IHC, WB, IP
Pricing
$595/0.1 mL | $2,535/5x0.1 mL
Testing Data
(Image: E18 hippocampal neurons stained with MAPT (red) and Doublecortin (green). The two proteins overlap in the proximal dendrites, but doublecortin is more abundant in the growth cones and periphery. As a result, the periphery appears green while the more proximal regions of the cells are yellow. The single longer process of this cell, presumably an axon, has a low doublecortin content and so appears red. Blue staining is the nuclear DNA. Protocol on datasheet.)
Doublecortin/DCX, Antibody (Cat# AAA555045)
Full Name
Doublecortin/DCX, Monoclonal Antibody, Mouse
Gene Names
DCX; DC; DBCN; LISX; SCLH; XLIS
Reactivity
Bovine, Human, Mouse, Porcine, Rat
Applications
IF, WB
Pricing
$435/0.1 mL | $1,815/5x0.1 mL
Testing Data
(Image: Rat brain neural cultures stained with FOX3/NeuN (red), and our chicken polyclonal antibody to GFAP (green) and DNA (blue). The FOX3 antibody reveals strong nuclear and distal cytoplasmic staining for Fox3/NeuN and the complete absence of staining of astrocytes, which are staining with the GFAP antibody, and other kinds of non-neuronal cells. Protocol on Data-sheet.)
Fox3/NeuN, Antibody (Cat# AAA555037)
Full Name
Fox3/NeuN, Monoclonal Antibody, Mouse
Reactivity
Bovine, Human, Mouse, Porcine, Rat
Applications
IF, WB
Pricing
$435/0.1 mL | $1,815/5x0.1 mL
Testing Data
(Image: Shows rat mixed neuron/glial cultures stained with Myelin Basic Protein antibody (red), and also with chicken antibody to neurofilament NF-L (green). Blue is a DNA stain. Note that the MBP antibody stains an oligodendrocyte and some membrane shed from this cell. Other cells in the field include neurons, astrocytes, microglia and fibroblasts, all of which are completely negative for MBP, though the neuronal processes can be seen with the NF-L antibody. Protocol on data-sheet.)
Myelin Basic Protein, Antibody (Cat# AAA555038)
Full Name
Myelin Basic Protein, Monoclonal Antibody, Mouse
Reactivity
Human, Rat
Applications
IF, WB
Pricing
$410/0.1 mL | $1,705/5x0.1 mL
Testing Data
(Image: Shows rat mixed neuron/glial cultures stained with chicken Myelin Basic Protein (green). Blue is a DNA stain. Note that the Myelin Basic Protein antibody stains an oligodendrocyte and some membrane shed from this cell. Other cells in the field include neurons, astrocytes, microglia and fibroblasts, all of which are completely negative. specifically, and that the staining is concentrated in the cell bodies. Protocol on data-sheet.)
Myelin Basic Protein, Antibody (Cat# AAA555394)
Full Name
Myelin Basic Protein, purified, Chicken IgY
Reactivity
Bovine, Human, Mouse, Rat
Applications
IF, WB
Pricing
$435/0.1 mL | $1,815/5x0.1 mL
Testing Data
(Image: ChAT staining of Human brain, basal ganglia. Note cytoplasmic staining of neurons of the nucleus basalis of Meynert. Paraffin section.)
ChaT, Antibody (Cat# AAA555072)
Full Name
ChaT, Monoclonal Antibody, Mouse
Reactivity
Human
Applications
IHC
Pricing
$290/0.1 mL | $1,155/5x0.1 mL
Testing Data
(Image: A tissue section through an adult mouse brain showing CNPase (brown staining) in white matter tracts and the granule cell layer of the cerebellum.)
CNPase, Antibody (Cat# AAA555380)
Full Name
CNPase, Affinity Purified Antibody, Chicken
Reactivity
Human, Mouse
Applications
ICC, IHC
Pricing
$210/0.1 mL | $800/5x0.1 mL
Testing Data
(Image: PGP9.5 175-191 staining of the synovium from ASIC3 +/+ mice with joint inflammation. Bar = 25 ?m. doi:10.1016/j.pain.2008.01.020.)
PGP9.5 (175-191), Antibody (Cat# AAA555356)
Full Name
PGP9.5 (175-191) Antiserum, guinea pig
Reactivity
Human, Mouse, Rat
Applications
IHC
Pricing
$275/0.05 mL | $510/0.15 mL | $2,140/5x0.15 mL
Testing Data
(Image: Mixed neuron-glial cultures stained with rabbit antibody to alpha-internexin (red) and chicken antibody to peripherin (green). The alpha internexin antibody stains numerous axonal and dendritic profiles in these cultures, while peripherin antibody binds to only a subset of neurons. Protocol on data-sheet)
Neurofilament alpha-internexin/NF66, Antibody (Cat# AAA555198)
Full Name
Neurofilament alpha-internexin/NF66, Polyclonal Antiserum, Rabbit
Reactivity
Human, Mouse, Rat
Applications
IF, WB
Pricing
$410/0.1 mL | $1,705/5x0.1 mL
Testing Data
(Image: View of mixed neuron/glial cultures stained with our MARCKS antibody (red) and MAP2 made in chicken. Note that the MARCKS antibody stains vesicular structures both in the glial cells and in the dendrites of the neurons, which are strongly stained with the MAP2 antibody. Protocol on data-sheet.More Links:)
MARCKS (Myristoylated Alanine Rich C Kinase Substrate), Antibody (Cat# AAA555192)
Full Name
MARCKS (Myristoylated Alanine Rich C Kinase Substrate), Antiserum, Rabbit
Gene Names
MARCKS; MACS; 80K-L; PKCSL; PRKCSL
Reactivity
Mouse, Rat
Applications
ICC, IF, WB
Pricing
$410/0.1 mL | $1,705/5x0.1 mL
Testing Data
(Image: NF-H-positive cultured neuron (green) from a neonatal mouse brain. Protocol on datasheet)
NF-H (Neurofilament, 200 kDa subunit), Antibody (Cat# AAA555378)
Full Name
NF-H (Neurofilament, 200 kDa subunit), Affinity Purified Antibody, Chicken
Reactivity
Human, Mouse
Applications
IHC
Pricing
$210/0.1 mL | $800/5x0.1 mL
Testing Data
(Image: The PGP mouse that we received from Neuromics works fantastically! I used it at 1:100 with Cy3 at 1:500 and got great staining with good background levels. PGP9.5 staining of Rat Abdominal Tissue. Courtesy of Jamie Nugent (Dr. Marna Ericson's Lab), University of Minnesota.)
PGP9.5, Antibody (Cat# AAA555024)
Full Name
PGP9.5, Monoclonal Antibody, Mouse
Reactivity
Bovine, Human, Mouse, Porcine, Rat
Applications
IHC, WB
Pricing
$655/0.1 mL | $2,800/5x0.1 mL
Testing Data
(Image: SynCAM Western Blotting in rat brain and HEK cell lysates showing different glycosylation patterns. Brain fractions give banding patterns of a core glycosylation population around 50kDa and a slightly higher glycosylated population around 65 kDa. The heterologous HEK cell lysates give a single, fully glycosylated, band around 107kDa.)
CADM1, Antibody (Cat# AAA555107)
Full Name
CADM1, Antiserum, Rabbit
Gene Names
CADM1; BL2; ST17; IGSF4; NECL2; RA175; TSLC1; IGSF4A; Necl-2; SYNCAM; sgIGSF; sTSLC-1; synCAM1
Reactivity
Mouse
Applications
IF, IHC
Pricing
$425/0.1 mL | $1,760/5x0.1 mL
Testing Data
(Image: Mixed neuron/glia cultures stained with MAP-2 (green) and also rabbit antibody of neurofilament NF-H (red). Since the NF-H protein is largely expressed in neuronal axons, while the MAP2 is only found in neuronal dendrites and perikarya, there is little overlap between these two staining patterns. DNA stain shows nuclei of neurons and non-neuronal cells (blue).Protocol on data-sheet.)
MAP2, Antibody (Cat# AAA555043)
Full Name
MAP2, Monoclonal Antibody, Mouse
Gene Names
MAP2; MAP2A; MAP2B; MAP2C
Reactivity
Bovine, Human, Mouse, Rat
Applications
IF, WB
Pricing
$435/0.1 mL | $1,815/5x0.1 mL
Testing Data
(Image: Mixed neuron-glial cultures stained with alpha-II spectrin (green) and counterstained for DNA (blue). The alpha-II spectrin antibody stains numerous axonal and dendritic profiles in these cultures, and this image shows an optical section through a group of neuronal cell bodies. The antibody clearly reveals the submembraneous cytoskeleton. Since alpha-II spectrin is specific for neurons in the CNS, the glial cells in this culture are not recognized. This antibody also reveals the submembranous cytoskeleton of the axon. Protocol on data-sheet.)
a-II spectrin, Antibody (Cat# AAA555048)
Full Name
a-II spectrin, Monoclonal Antibody, Mouse
Reactivity
Human, Mouse, Rat
Applications
IF, WB
Pricing
$435/0.1 mL | $1,815/5x0.1 mL
Testing Data
PGP9.5, Antibody (Cat# AAA555088)
Full Name
PGP9.5, Monoclonal Antibody, Mouse
Reactivity
Human, Mouse, Rat
Applications
IHC, WB
Pricing
$560/0.1 mL | $2,365/5x0.1 mL
Testing Data
(Image: Rat brain neural cultures stained with FOX1 (green), and our rabbit polyclonal antibody to NF-M (green) and DNA (blue). This antibody reveals nuclear and distal cytoplasmic staining for Fox1, and the complete absence of staining of non-neuronal cells.Protocol on Data-sheet.)
FOX1, Antibody (Cat# AAA555031)
Full Name
FOX1, Monoclonal Antibody, Mouse
Reactivity
Bovine, Human, Mouse, Rat
Applications
IF, WB
Pricing
$435/0.1 mL | $1,815/5x0.1 mL
Testing Data
(Image: shows the human embryonic kidney cells line 293 (Hek293), which express many neuronal proteins (1). The red channel shows staining with NSE, which recognizes all of these 293 cells. The green channels shows staining for another neuronal marker with mouse monoclonal to ubiquitin C-terminal hydrolase 1 (UCHL1) antibody. This neuronal gene is apparently activated in a cell density dependent fashion and at this stage only a few cells express this protein. However all cells that express NSE also express UCHL1.)
NSE (Neuron Specific Enolase), Antibody (Cat# AAA555193)
Full Name
NSE (Neuron Specific Enolase), Antibody
Gene Names
ENO2; NSE
Reactivity
Human
Applications
IF, WB
Pricing
$410/0.1 mL | $1,705/5x0.1 mL
Testing Data
(Image: Detection of tyrosine hydroxylase in PC12 cell lysate by Western Blot. Band is at approximately 60 kDa.)
Tyrosine Hydroxylase, Antibody (Cat# AAA555098)
Full Name
Tyrosine Hydroxylase, Affinity Purified Antibody, Sheep
Gene Names
TH; TYH; DYT14; DYT5b
Reactivity
Human, Mouse, Primate, Rat
Applications
ICC, IF, IHC, WB
Pricing
$685/0.1 mL | $2,930/5x0.1 mL
Testing Data
GAP43, Antibody (Cat# AAA555188)
Full Name
GAP43, Affinity Purified Antibody, Rabbit
Reactivity
Bovine, Human, Mouse, Rat
Applications
IF, WB
Pricing
$435/0.1 mL | $1,815/5x0.1 mL
Testing Data
Alpha 2a Adrenergic Receptor, Antibody (Cat# AAA555609)
Full Name
Alpha 2a Adrenergic Receptor, Affinity Purified Antibody, Rabbit
Gene Names
ADRA2A; ADRA2; ADRAR; ZNF32; ADRA2R; ALPHA2AAR
Reactivity
Rat
Applications
IHC
Pricing
$285/0.05 mL | $570/0.15 mL | $2,410/5x0.15 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma, using Sodium Potassium ATPase Antibody(MBS179502)ATP1A1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ATP1A1 Antibody (MBS179502)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Sodium Potassium ATPase, Monoclonal Antibody (Cat# AAA179502)
Full Name
Anti-Sodium Potassium ATPase Rabbit Monoclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
FC, IHC, ICC, IF, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Testing Data
LepRb/OBRb, Antibody (Cat# AAA555607)
Full Name
LepRb/OBRb, blocking peptide
Reactivity
Human, Mouse, Rat
Applications
IHC
Pricing
$195/0.1 mg | $720/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rab4A expression in (1) SH-SY5Y cell lysate; (2) MCF-7 cell lysate (MBS1750026).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB4A monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RAB4A )
Rab4A/Rab4, Monoclonal Antibody (Cat# AAA1750026)
Full Name
Anti-Rab4A/Rab4 Rabbit Monoclonal Antibody
Gene Names
RAB4A; RAB4; HRES1; HRES-1; HRES-1/RAB4
Reactivity
Human, Mouse, Rat
Applications
IP, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Testing Data
(Image: Cochlear neurons in the cochlear ganglion of an adult mouse brain stained green with doublecortin. Neurofilament immunoreactivity stained red using a rabbit antibody.)
Doublecortin, Antibody (Cat# AAA555568)
Full Name
Doublecortin, Affinity Purified Antibody
Gene Names
DCX; DC; DBCN; LISX; SCLH; XLIS
Reactivity
Human, Mouse
Applications
ICC, IHC
Pricing
$210/0.1 mL | $800/5x0.1 mL
Testing Data
PGP9.5 175, Antibody (Cat# AAA555573)
Full Name
PGP9.5 175, Control Peptide
Reactivity
Human, Mouse, Rat
Applications
IHC
Pricing
$195/0.02 mg | $720/5x0.02 mg
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon, using Rab5 Antibody(MBS179758)RAB5A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-RAB5A Antibody (MBS179758)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Rab5, Monoclonal Antibody (Cat# AAA179758)
Full Name
Anti-Rab5 Rabbit Monoclonal Antibody
Gene Names
RAB5A; RAB5
Reactivity
Human, Mouse, Rat
Applications
IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Profilin1 expression in Jurkat cell lysate (MBS179670).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PFN1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PFN1 )
Profilin1, Monoclonal Antibody (Cat# AAA179670)
Full Name
Anti-Profilin1 Rabbit Monoclonal Antibody
Gene Names
PFN1; ALS18
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Piccolo Monoclonal Antibody, Clone 6H9-B6 . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Piccolo Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Endoplasmic Reticulum, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Piccolo Antibody. (D) Composite.)
Piccolo, Monoclonal Antibody (Cat# AAA8001940)
Full Name
Piccolo Antibody
Gene Names
Pclo; Acz; Pico
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$285/0.025 mg | $1,055/5x0.025 mg
Western Blot (WB)
(Western blot analysis of Rab4 expression in (1)MCF7 cell lysates (2)HeLa cell lysate (MBS1750027).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB4A monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RAB4A )
Rab4, Monoclonal Antibody (Cat# AAA1750027)
Full Name
Anti-Rab4 Rabbit Monoclonal Antibody
Gene Names
RAB4A; RAB4; HRES1; HRES-1; HRES-1/RAB4
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
