Testing Data
(Image: Mixed neuron-glial cultures stained with alpha-II spectrin (green) and counterstained for DNA (blue). The alpha-II spectrin antibody stains numerous axonal and dendritic profiles in these cultures, and this image shows an optical section through a group of neuronal cell bodies. The antibody clearly reveals the submembraneous cytoskeleton. Since alpha-II spectrin is specific for neurons in the CNS, the glial cells in this culture are not recognized. This antibody also reveals the submembranous cytoskeleton of the axon. Protocol on data-sheet.)
a-II spectrin Antibody
a-II spectrin, Monoclonal Antibody, Mouse
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Western Blot
Synonyms
a-II spectrin; Monoclonal Antibody; Mouse; anti-a-II spectrin antibody
Reactivity
Human, Mouse, Rat
Form/Format
Affinity Purified - Liquid
Applicable Applications for anti-a-II spectrin antibody
Immunofluorescence (IF), Western Blot (WB)
Type Info
Mouse IgG
Immunogen
Recombinant protein construct containing the 7th, 8th and 9th spectrin repeats of human alpha-II spectrin (amino acids 676-1043. This construct was expressed in and purified from E Coli.
Testing Data
(Image: Mixed neuron-glial cultures stained with alpha-II spectrin (green) and counterstained for DNA (blue). The alpha-II spectrin antibody stains numerous axonal and dendritic profiles in these cultures, and this image shows an optical section through a group of neuronal cell bodies. The antibody clearly reveals the submembraneous cytoskeleton. Since alpha-II spectrin is specific for neurons in the CNS, the glial cells in this culture are not recognized. This antibody also reveals the submembranous cytoskeleton of the axon. Protocol on data-sheet.)
Testing Data
(Image: Mixed neuron-glial cultures stained with alpha-II spectrin (green) and counterstained for DNA (blue). The alpha-II spectrin antibody stains numerous axonal and dendritic profiles in these cultures, and this image shows an optical section through a group of neuronal cell bodies. The antibody clearly reveals the submembraneous cytoskeleton. Since alpha-II spectrin is specific for neurons in the CNS, the glial cells in this culture are not recognized. This antibody also reveals the submembranous cytoskeleton of the axon. Protocol on data-sheet.)
Testing Data
(Image: Western blots of extract of bovine spinal cord. A prominent band running at 240kDa respresents the intact alpha-II spectrin heavy chain. Protocol on data-sheet..)
Testing Data
(Image: Western blots of extract of bovine spinal cord. A prominent band running at 240kDa respresents the intact alpha-II spectrin heavy chain. Protocol on data-sheet..)
Related Product Information for anti-a-II spectrin antibody
The spectrin family of proteins were originally discovered as major components of the submembraneous cytoskeleton of osmotically lyzed red blood cells. Work by Levine and Willard described a pair of about ~240-260kDa molecular weight bands which were transported at the slowest rate along mammalian axons. They named these proteins "fodrin" as antibody studies showed that they were localized in the sheath under the axonal membrane, but not in the core of the axon.
Product Categories/Family for anti-a-II spectrin antibody
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Product Notes
The a-II spectrin (Catalog #AAA555048) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The a-II spectrin, Monoclonal Antibody, Mouse reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's a-II spectrin can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB). Researchers should empirically determine the suitability of the a-II spectrin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "a-II spectrin, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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