Western Blot (WB)
(WDR5B Antibody (N-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the WDR5B antibody detected the WDR5B protein (arrow).)
Rabbit anti-Human WDR5B Polyclonal Antibody | anti-WDR5B antibody
WDR5B, NT (WDR5B, WD repeat-containing protein 5B)
Reactivity
Human
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
WDR5B; Polyclonal Antibody; NT (WDR5B; WD repeat-containing protein 5B); Anti -WDR5B; anti-WDR5B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-WDR5B antibody
ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50
Immunogen
WDR5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-85 amino acids from the N-terminal region of human WDR5B.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(WDR5B Antibody (N-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the WDR5B antibody detected the WDR5B protein (arrow).)
Western Blot (WB)
(WDR5B Antibody (N-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the WDR5B antibody detected the WDR5B protein (arrow).)
Flow Cytometry (FC/FACS)
(WDR5B Antibody (N-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(WDR5B Antibody (N-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-WDR5B antibody
This intronless gene encodes a protein containing several WD40 repeats. WD repeats are approximately 30- to 40-amino acid domains containing several conserved residues, including a trp-asp at the C-terminal end. The encoded protein may mediate protein-protein interactions.
Product Categories/Family for anti-WDR5B antibody
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
36,338 Da
NCBI Official Full Name
WDR5B
UniProt Protein Name
WD repeat-containing protein 5B
Protein Family
UniProt Gene Name
WDR5B
UniProt Entry Name
WDR5B_HUMAN
Uniprot Description
Function: May function as a substrate receptor for CUL4-DDB1 ubiquitin E3 ligase complex
By similarity.
Subunit structure: Probable part of a cullin-RING E3 protein ligase complex containing CUL4B-DDB1 and a substrate-recruiting component (DCAF). Interacts with CUL4B and DDB1
By similarity.
Sequence similarities: Belongs to the WD repeat WDR5/wds family.Contains 7 WD repeats.
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Product Notes
The WDR5B wdr5b (Catalog #AAA647797) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The WDR5B, NT (WDR5B, WD repeat-containing protein 5B) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's WDR5B can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the WDR5B wdr5b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WDR5B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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