Mouse PSD95 Monoclonal Antibody | anti-Dlg4 antibody

PSD95 Antibody

Cat. Num. AAA804156

• Gene Names: Dlg4; Dlgh4; PSD95; Sap90
• Reactivity: Mouse, Rat, Bovine, Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Synonyms: PSD95; Monoclonal Antibody; PSD95 Antibody; PSD 95; PSD-95; DLG4; SAP90; Synapse-associated protein 90; Postsynaptic density protein 95; Disks large homolog 4; anti-Dlg4 antibody

• Host: Mouse
• Reactivity: Mouse, Rat, Bovine, Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 7E3
• Specificity: Detects ~100kDa. An additional protein of >100kDa is also detected. Additional cross-reactive bands are detected at ~75kDa and 50kDa in rat and mouse samples.
• Form/Format: Protein G Purified; PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 724

• Applicable Applications for anti-Dlg4 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Antibody Microarray (AM)
• Application Notes: WB: 1:1000, IHC: 1:1000, ICC/IF: 1:100; Optimal dilutions for assays should be determined by end users.
• Certificate of Analysis: 1ug/ml was sufficient for detection of PSD-95 on 20ug rat brain tissue extract by ECL immunoblot analysis using Goat Anti-Mouse (No Suggestions) as the secondary.
• Research Areas: Cell Markers, Cell Signaling, Neuron Markers, Neuroscience, Organelle Markers, Postsynaptic Markers
• Cellular Localization: Axon, Cell Junction, Cell membrane, Cell projection, Postsynaptic cell membrane, Postsynaptic density, Synapse
• Preparation and Storage: Store at -20 degree C.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 7E3. Tissue: Neocortex. Species: Rat. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:1000.)

Immunocytochemistry/Immunofluorescence (ICC/IF)

(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 7E3. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filamentous-like staining.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 7E3. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Basal cell staining in the epidermis, some hair follicle staining, dermal staining. Backskin obtained from transgenic mice.)

Western Blot (WB)

(Western Blot analysis of Rat brain membrane lysate showing detection of PSD95 protein using Mouse Anti-PSD95 Monoclonal Antibody, Clone 7E3. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:1000.)

Related Product Information for anti-Dlg4 antibody

Background Info: Detects a ~100kDa protein corresponding to the molecular mass of PSD-95 on SDS PAGE immunoblots. Additional cross-reactive bands are detected at ~80kDa and ~50kDa in rat and mouse samples.

Scientific Background: Postsynaptic Density protein 95 (PSD95), also known as Synapse associated protein 90kDa, is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. PSD95 is a scaffolding protein and is involved in the assembly and function of the postsynaptic density complex (1). These family members consist of an N-terminal variable segment followed by three amino-terminal PDZ domains, an upstream SH3 domain and an inactive carboxyl-terminal guanylate kinase (GK) domain. The first and second PDZ domain localize NMDA receptors and K+ channels to synapses, and the third binds to neuroligins which are neuronal cell adhesion molecules that interact with b-neurexins and form intercellular junctions. PSD-95 also binds to neuronal nitric oxide synthase, possibly through interactions between PDZ domains present on both proteins (2). Thus different PDZ domains of PSD-95 might be specialized for distinct functions (3, 4). PSD95 participates in synaptic targeting of AMPA receptors through an indirect manner involving Stargazin and related transmembrane AMPA receptor regulatory proteins (TARPs) (5). The protein is implicated in experience dependent plasticity and plays an indispensable role in learning (6). Mutations in PSD95 are associated with autism (7).

Product Categories/Family for anti-Dlg4 antibody

Neuroscience; Organelle Markers

References

1. Chetkovich D.M., Bunn R.C., Kuo S.H., Kawasaki Y., Kohwi M., and Bredt D.S. (2002) J Neurosci. 22(15): 6415-25. 2. Cao J., Viholainen J.I., Dart C., Warwick H.K., Levland M.L. and Courtney M.J. (2005) J Cell Biol. 168(1): 117-26. 3. Kennedy M. (1997) Trends in Neurosci. 6: 264-268. 4. Irie M. et al. (1997) Science 277(5331): 1511-5. 5. Cai C. et al. (2006) J Biol Chem. 281: 4267-73. 6. Yao W.D. et al. (2004) Neuron 41: 625-38. 7. Cline H. (2005) Curr Biol. 15: R203-5.

NCBI and Uniprot Product Information

• NCBI GI #: 9665227
• NCBI GeneID: 29495
• NCBI Accession #: NP_062567.1
• NCBI GenBank Nucleotide #: NM_019621.1
• UniProt Accession #: P31016; P97631
• NCBI Official Full Name: disks large homolog 4
• NCBI Official Synonym Full Names: discs, large homolog 4 (Drosophila)
• NCBI Official Symbol: Dlg4
• NCBI Official Synonym Symbols: Dlgh4; PSD95; Sap90
• NCBI Protein Information: disks large homolog 4; PSD-95; SAP-90; discs large homolog 4; synapse-associated protein 90; postsynaptic density protein 95; synapse-associated protein SAP90
• UniProt Protein Name: Disks large homolog 4
• Protein Family: Disks large
• UniProt Gene Name: Dlg4
• UniProt Synonym Gene Names: Dlgh4; Psd95; PSD-95; SAP-90; SAP90
• UniProt Entry Name: DLG4_RAT

NCBI Description

a molecular scaffolding protein that binds and clusters N-methyl-D-aspartate receptors at neuronal synapses; may be involved in guanine nucleotide-mediated signal transduction pathway [RGD, Feb 2006]

Uniprot Description

PSD-95: a membrane associated guanylate kinase (MAGUK) scaffolding protein located in neural postsynaptic densities. Associates with NMDA receptor NR2 subunits via glutamate serine (aspartate/glutamate) valine motifs in the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Its overexpression or depletion changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells. May reduce the amplitude of ACCN3 acid-evoked currents by retaining the channel intracellularly. Binds tissue-type plasminogen activator (tPA), mediating neural NMDA gating via a complex of LRP1, PSD-95 and NMDAR. Interacts through its first two PDZ domains with NMDAR2A, NMDAR2B, NMDAR2C, NMDAR2D, ACCN3, certain splice forms of NMDAR1, KV4.2, CXADR and synGAP. Interacts through its first two PDZ domains with Kv1.1, Kv1.2, Kv1.3, Kv1.4 and HER4. Interacts through its first PDZ domain with GluR6 and Kv1.4. Interacts through its second PDZ domain with the PDZ domain of nNOS or the C-terminus of NOS1AP. May interact with 5-HT(2A). Interacts through its third PDZ domain with NLGN1, and probably with NLGN2 and NLGN3. Interacts through its guanylate kinase-like domain with SAPAP1, SAPAP2, SAPAP3, SAPAP4, MAP1A, and BEGAIN. Interacts through its guanylate kinase-like domain with KIF13B. Two alternatively spliced human isoforms have been reported. Palmitoylation of isoform 1 is required for targeting to postsynaptic density.

Protein type: Adaptor/scaffold

Cellular Component: dendrite cytoplasm; cortical cytoskeleton; voltage-gated potassium channel complex; endoplasmic reticulum; basolateral plasma membrane; dendrite; postsynaptic density; dendritic spine; excitatory synapse; postsynaptic membrane; synaptic vesicle; extrinsic to internal side of plasma membrane; membrane; cytoplasm; plasma membrane; ionotropic glutamate receptor complex; synapse; cell junction

Molecular Function: protein C-terminus binding; P2Y1 nucleotide receptor binding; glutamate receptor binding; beta-1 adrenergic receptor binding; acetylcholine receptor binding; protein phosphatase binding; protein kinase binding; PDZ domain binding; ionotropic glutamate receptor binding; guanylate kinase activity; protein binding; protein complex binding; D1 dopamine receptor binding; receptor binding

Biological Process: regulation of long-term neuronal synaptic plasticity; establishment and/or maintenance of epithelial cell polarity; elevation of cytosolic calcium ion concentration; establishment of protein localization; synaptic vesicle maturation; protein complex assembly; social behavior; neuronal ion channel clustering; neuromuscular process controlling balance; positive regulation of synaptic transmission; negative regulation of receptor internalization; nucleotide phosphorylation