Mouse Piccolo Monoclonal Antibody | anti-Pclo antibody

Piccolo Antibody

Cat. Num. AAA8001940

• Gene Names: Pclo; Acz; Pico
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, ELISA
• Purity: Protein G Purified
• Synonyms: Piccolo; Monoclonal Antibody; Piccolo Antibody; Mouse Anti-Mouse Piccolo Monoclonal IgG2b Kappa; Clone 6H9-B6; ACZ Antibody; Aczonin Antibody; PCLO Antibody; Pico Antibody; presynaptic cytomatrix protein Antibody; anti-Pclo antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2b Kappa
• Clone Number: 6H9-B6
• Specificity: Detects ~550kDa. No cross-reactivity to Bassoon.
• Purity/Purification: Protein G Purified
• Form/Format: PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 4863

• Applicable Applications for anti-Pclo antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: WB: 1:1000; ICC/IF: 1:100
• Immunogen: Full length recombinant protein (GenBank ID: BC001304.1)
• Immunogen Species: Mouse
• Conjugate: Unconjugated
• Cellular Localization: Cell Junction, Synapse
• Certificate of Analysis: 1 ug/ml of SMC-188 was sufficient for detection of Piccolo in 20 ug of rat brain tissue lysate by colorimetric immunoblot analysis using goat anti mouse IgG:HRP as the secondary antibody.
• Preparation and Storage: Store at-20 degree C.

Immunocytochemistry (ICC)

(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Piccolo Monoclonal Antibody, Clone 6H9-B6 . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Piccolo Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Endoplasmic Reticulum, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Piccolo Antibody. (D) Composite.)

Western Blot (WB)

(Western Blot analysis of Rat and mouse brain lysates showing detection of Piccolo protein using Mouse Anti-Piccolo Monoclonal Antibody, Clone 6H9-B6 . Primary Antibody: Mouse Anti-Piccolo Monoclonal Antibody at 1:1000. (1) Mouse brain lysate; (2) Rat brain lysate.)

Related Product Information for anti-Pclo antibody

Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains and C-terminal PDZ and C2 domains (1, 2). In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones) (3). Piccolo is a scaffolding protein for proteins involved in endo-and exocytosis of synaptic vesicles. Piccolo has also been shown to interfere with clathrin medicated endocytosis by binding to the F-actin and dynamin binding protein Abp1 (2).

Product Categories/Family for anti-Pclo antibody

Neuroscience; Cell Markers; Neuron Markers; Presynaptic Markers

References

1. Gerber S.H., Garcia J., Rizo J., Sudhof T.C. (2001) EMBO J. 20(7): 1605-1619. 2. Wang X., et al. (1999) J Cell Biol. 147(1): 151-162. 3. Dresbach T., et al. (2006) J Biol Chem. 281(9):6038-6047.

NCBI and Uniprot Product Information

• NCBI GI #: 160707978
• NCBI GeneID: 26875
• NCBI Accession #: NP_001104266.1
• NCBI GenBank Nucleotide #: NM_001110796.2
• UniProt Accession #: Q9QYX7
• NCBI Official Full Name: protein piccolo isoform 2
• NCBI Official Synonym Full Names: piccolo (presynaptic cytomatrix protein)
• NCBI Official Symbol: Pclo
• NCBI Official Synonym Symbols: Acz; Pico
• NCBI Protein Information: protein piccolo
• UniProt Protein Name: Protein piccolo
• Protein Family: Protein piccolo
• UniProt Gene Name: Pclo
• UniProt Synonym Gene Names: Acz
• UniProt Entry Name: PCLO_MOUSE

Uniprot Description

piccolo: May act as a scaffolding protein involved in the organization of synaptic active zones and in synaptic vesicle trafficking. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Cellular Component: cell; postsynaptic density; synapse; intracellular; cell junction

Molecular Function: protein binding; calcium-dependent phospholipid binding; metal ion binding; calcium ion binding; profilin binding

Biological Process: regulation of exocytosis; cAMP-mediated signaling; insulin secretion; cytoskeleton organization and biogenesis; synaptic vesicle targeting