Mouse PSD95 Monoclonal Antibody | anti-PSD95 antibody

PSD95 Antibody

Cat. Num. AAA8001880

• Gene Names: Dlg4; Dlgh4; PSD95; Sap90
• Reactivity: Human, Mouse, Rat, Bovine
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein G Purified
• Synonyms: PSD95; Monoclonal Antibody; PSD95 Antibody; Mouse Anti-Rat PSD95 Monoclonal IgG1; PSD 95 Antibody; Clone 7E3; PSD-95 Antibody; DLG4 Antibody; SAP90 Antibody; Synapse-associated protein 90 Antibody; Postsynaptic density protein 95 Antibody; Disks large homolog 4 Antibody; anti-PSD95 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Bovine
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 7E3
• Specificity: Detects ~100kDa. An additional protein of >100kDa is also detected. Additional cross-reactive bands are detected at ~75kDa and 50kDa in rat and mouse samples.
• Purity/Purification: Protein G Purified
• Form/Format: PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 724

• Applicable Applications for anti-PSD95 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Antibody Microarray (AM)
• Application Notes: WB: 1:1000; IHC: 1:1000; ICC/IF: 1:100
• Immunogen: Recombinant rat PSD-95
• Immunogen Species: Rat
• Conjugate: Unconjugated
• Cellular Localization: Cell Membrane, Cell Junction, Synapse, Postsynaptic Cell Membrane, Postsynaptic Density, Cell Projection, Axon
• Certificate of Analysis: 1 ug/ml was sufficient for detection of PSD-95 on 20 ug rat brain tissue extract by ECL immunoblot analysis using Goat Anti-Mouse IgG: HRP as the secondary.
• Preparation and Storage: Store at-20 degree C.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 7E3 . Tissue: Neocortex. Species: Rat. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:1000.)

Immunocytochemistry (ICC)/Immunofluorescence (IF)

(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 7E3 . Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at-20 degree C. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filamentous-like staining.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 7E3 . Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Basal cell staining in the epidermis, some hair follicle staining, dermal staining. Backskin obtained from transgenic mice.)

Western Blot (WB)

(Western Blot analysis of Rat brain membrane lysate showing detection of PSD95 protein using Mouse Anti-PSD95 Monoclonal Antibody, Clone 7E3 . Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:1000.)

Related Product Information for anti-PSD95 antibody

Postsynaptic Density protein 95 (PSD95), also known as Synapse associated protein 90kDa, is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. PSD95 is a scaffolding protein and is involved in the assembly and function of the postsynaptic density complex (1). These family members consist of an N-terminal variable segment followed by three amino-terminal PDZ domains, an upstream SH3 domain and an inactive carboxyl-terminal guanylate kinase (GK) domain. The first and second PDZ domain localize NMDA receptors and K+ channels to synapses, and the third binds to neuroligins which are neuronal cell adhesion molecules that interact with b-neurexins and form intercellular junctions. PSD-95 also binds to neuronal nitric oxide synthase, possibly through interactions between PDZ domains present on both proteins (2). Thus different PDZ domains of PSD-95 might be specialized for distinct functions (3, 4). PSD95 participates in synaptic targeting of AMPA receptors through an indirect manner involving Stargazin and related transmembrane AMPA receptor regulatory proteins (TARPs) (5). The protein is implicated in experience dependent plasticity and plays an indispensable role in learning (6). Mutations in PSD95 are associated with autism (7).

Product Categories/Family for anti-PSD95 antibody

Neuroscience; Cell Markers; Neuron Markers; Postsynaptic Markers; Cell Signaling; Organelle Markers

References

1. Chetkovich D.M., Bunn R.C., Kuo S.H., Kawasaki Y., Kohwi M., and Bredt D.S. (2002) J Neurosci. 22(15): 6415-25. 2. Cao J., Viholainen J.I., Dart C., Warwick H.K., Levland M.L. and Courtney M.J. (2005) J Cell Biol. 168(1): 117-26. 3. Kennedy M. (1997) Trends in Neurosci. 6: 264-268. 4. Irie M. et al. (1997) Science 277(5331): 1511-5. 5. Cai C. et al. (2006) J Biol Chem. 281: 4267-73. 6. Yao W.D. et al. (2004) Neuron 41: 625-38. 7. Cline H. (2005) Curr Biol. 15: R203-5.

NCBI and Uniprot Product Information

• NCBI GI #: 9665227
• NCBI GeneID: 29495
• NCBI Accession #: NP_062567.1
• NCBI GenBank Nucleotide #: NM_019621.1
• UniProt Accession #: P31016
• NCBI Official Full Name: disks large homolog 4
• NCBI Official Synonym Full Names: discs large MAGUK scaffold protein 4
• NCBI Official Symbol: Dlg4
• NCBI Official Synonym Symbols: Dlgh4; PSD95; Sap90
• NCBI Protein Information: disks large homolog 4
• UniProt Protein Name: Disks large homolog 4
• UniProt Gene Name: Dlg4
• UniProt Synonym Gene Names: Dlgh4; Psd95; PSD-95; SAP-90; SAP90

NCBI Description

a molecular scaffolding protein that binds and clusters N-methyl-D-aspartate receptors at neuronal synapses; may be involved in guanine nucleotide-mediated signal transduction pathway [RGD, Feb 2006]

Uniprot Description

Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.