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Western Blot (WB) (All lanes : Anti-PLIN3 Antibody at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: K562 whole cell lysateLane 3: Daudi whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Mouse anti-Human PLIN3 Monoclonal Antibody | anti-PLIN3 antibody

PLIN3 Antibody

Gene Names
PLIN3; PP17; TIP47; M6PRBP1
Reactivity
Human
Applications
Western Blot, ELISA
Synonyms
PLIN3; Monoclonal Antibody; PLIN3 Antibody; Perilipin-3; 47 kDa mannose 6-phosphate receptor-binding protein; 47 kDa MPR-binding protein; Cargo selection protein TIP47; Mannose-6-phosphate receptor-binding protein 1; Placental protein 17; PP17; M6PRBP1; TIP47; anti-PLIN3 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a,k
Specificity
This PLIN3 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 1-434 amino acids from human PLIN3.
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Sequence Length
433
Applicable Applications for anti-PLIN3 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB:~~1:2000
Subtitle
Purified Mouse Monoclonal Antibody (Mab)
Cellular Location
Cytoplasm. Endosome membrane; Peripheral membrane protein; Cytoplasmic side. Lipid droplet. Note=Membrane associated on endosomes. Detected in the envelope and the core of lipid bodies and in lipid sails.
Antigen Type
Recombinant Protein
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-PLIN3 Antibody at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: K562 whole cell lysateLane 3: Daudi whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (All lanes : Anti-PLIN3 Antibody at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: K562 whole cell lysateLane 3: Daudi whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(All lanes : Anti-PLIN3 Antibody at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: K562 whole cell lysateLane 3: Daudi whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (All lanes : Anti-PLIN3 Antibody at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: K562 whole cell lysateLane 3: Daudi whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(All lanes : Anti-PLIN3 Antibody at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: K562 whole cell lysateLane 3: Daudi whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (All lanes : Anti-PLIN3 Antibody at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: K562 whole cell lysateLane 3: Daudi whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-PLIN3 antibody
Required for the transport of mannose 6-phosphate receptors (MPR) from endosomes to the trans-Golgi network.
Product Categories/Family for anti-PLIN3 antibody
References
Diaz E.,et al.Cell 93:433-443 (1998). Than N.G.,et al.Eur. J. Biochem. 258:752-757 (1998). Than N.G.,et al.Tumor Biol. 20:184-192 (1999). Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases. Ota T.,et al.Nat. Genet. 36:40-45 (2004).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
47075 Da
NCBI Official Full Name
perilipin-3 isoform 2
NCBI Official Synonym Full Names
perilipin 3
NCBI Official Symbol
PLIN3
NCBI Official Synonym Symbols
PP17; TIP47; M6PRBP1
NCBI Protein Information
perilipin-3
UniProt Protein Name
Perilipin-3
Protein Family
UniProt Gene Name
PLIN3
UniProt Synonym Gene Names
M6PRBP1; TIP47; 47 kDa MPR-binding protein; PP17
UniProt Entry Name
PLIN3_HUMAN

NCBI Description

Mannose 6-phophate receptors (MPRs) deliver lysosomal hydrolase from the Golgi to endosomes and then return to the Golgi complex. The protein encoded by this gene interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs, and is required for endosome-to-Golgi transport. This protein also binds directly to the GTPase RAB9 (RAB9A), a member of the RAS oncogene family. The interaction with RAB9 has been shown to increase the affinity of this protein for its cargo. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2009]

Uniprot Description

perilipin 3: a membrane-associated protein and a coat protein for lipid droplets (LDs). It has been described as a mannose-6-phosphate receptor (MPR) binding protein required for endosome-to-Golgi transport of MPRs, but this function has been disputed. Interacts with M6PR and IGF2R via their cytoplasmic domains. Affects hepatic lipid and glucose metabolism. Belongs to the perilipin family. 3 isoforms of the human protein are produced by alternative splicing. Isoform 2 may exist as a homodimer known as PP17C.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cytoplasm; cytosol; endosome; endosome membrane; Golgi apparatus; intracellular membrane-bound organelle; lipid particle; membrane; transport vesicle

Molecular Function: protein binding

Biological Process: vesicle-mediated transport

Research Articles on PLIN3

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Product Notes

The PLIN3 plin3 (Catalog #AAA9217030) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PLIN3 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PLIN3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB:~~1:2000. Researchers should empirically determine the suitability of the PLIN3 plin3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLIN3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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