CDH1 / E Cadherin Polyclonal Antibody | anti-CDH1 antibody

Anti-CDH1 / E Cadherin Antibody IHC-plus

Cat. Num. AAA249742

• Gene Names: CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; CD324
• Reactivity: Mouse, Dog, Rat, Human, Monkey
• Applications: Immunohistochemistry, Immunohistochemistry, Western Blot
• Purity: Immunoaffinity purified
• Synonyms: CDH1 / E Cadherin; Polyclonal Antibody; Anti-CDH1 / E Cadherin Antibody IHC-plus; Goat Polyclonal to Human CDH1 / E Cadherin; Human CDH1 / E Cadherin; CD324; CDHE; Cell-CAM 120/80; E Cadherin; E-Cadherin; ECAD; Epithelial cadherin; Arc-1; UVO; Cadherin-1; CAM 120/80; CD324 antigen; LCAM; Uvomorulin; anti-CDH1 antibody

• Host: CDH1/E Cadherin antibody was produced in Goat.
• Reactivity: Mouse, Dog, Rat, Human, Monkey
• Clonality: Polyclonal
• Specificity: Detects endogenous levels of total E-cadherin protein by Western blot in the whole cell lysates (e.g. HeLa and COS-7). This Ab does not recognize CDH2.
• Purity/Purification: Immunoaffinity purified
• Form/Format: PBS, 0.05% Sodium Azide, 20% Glycerol.
• Concentration: 3 mg/ml (varies by lot)
• Sequence Length: 882

• Applicable Applications for anti-CDH1 antibody: Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC-P (5 ug/ml); WB (1:500-1:2000).; ; Optimal dilution to be determined by the researcher.
• Family/Subfamily: Cadherin/not assigned-Cadherin
• Immunogen: CDH1 / E Cadherin antibody was raised against recombinant peptide derived from within residues 601 to 701 of human CDH1 produced in E Coli.
• Immunogen Species: CDH1/E Cadherin antibody was raised against Human
• Target: CDH1/E Cadherin
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening.
• Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.
• Preparation and Storage: Store at 4°C for one month or -20°C for longer. Avoid freeze/thaw cycles.

Immunohistochemistry - Paraffin (IHC)

(Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry - Paraffin (IHC)

(Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(Endogenous CDH1 detected with Anti-CDH1 Ab at 1:500 dilution. Lysate at 100ug per lane.)

Related Product Information for anti-CDH1 antibody

CDH1 Antibody, CD324 Antibody, CDHE Antibody, Cell-CAM 120/80 Antibody, E Cadherin Antibody, E-Cadherin Antibody, ECAD Antibody, Epithelial cadherin Antibody, Arc-1 Antibody, UVO Antibody, Cadherin-1 Antibody, CAM 120/80 Antibody, CD324 antigen Antibody, LCAM Antibody, Uvomorulin Antibody Description: CDH1 / E Cadherin is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis.

NCBI and Uniprot Product Information

• NCBI GI #: 4757960
• NCBI GeneID: 999
• NCBI Accession #: NP_004351.1
• NCBI GenBank Nucleotide #: NM_004360.4
• UniProt Accession #: P12830; Q13799; Q14216; Q15855; Q16194; Q4PJ14; Q9UII8; A8K1U7
• NCBI Official Full Name: cadherin-1 isoform 1 preproprotein
• NCBI Official Synonym Full Names: cadherin 1
• NCBI Official Symbol: CDH1
• NCBI Official Synonym Symbols: UVO; CDHE; ECAD; LCAM; Arc-1; CD324
• NCBI Protein Information: cadherin-1
• UniProt Protein Name: Cadherin-1
• Protein Family: Cadherin
• UniProt Gene Name: CDH1
• UniProt Synonym Gene Names: CDHE; UVO; E-cadherin
• UniProt Entry Name: CADH1_HUMAN

NCBI Description

This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015]

Uniprot Description

CDH1: a single-pass type I membrane protein, and calcium dependent cell adhesion proteins. It is a ligand for integrin alpha-E/beta-7, and it colocalizes with DLG7 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Defects in CDH1 are involved in dysfunction of the cell-cell adhesion system, triggering cancer invasion (gastric, breast, ovary, endometrium and thyroid) and metastasis. Has a potent invasive suppressor role.

Protein type: Cell adhesion; Ubiquitin ligase; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: actin cytoskeleton; apical junction complex; catenin complex; cell junction; cell-cell adherens junction; cortical actin cytoskeleton; cytoplasm; endosome; extracellular region; flotillin complex; focal adhesion; integral to membrane; internal side of plasma membrane; lamellipodium; lateral plasma membrane; perinuclear region of cytoplasm; plasma membrane; trans-Golgi network

Molecular Function: ankyrin binding; beta-catenin binding; calcium ion binding; cell adhesion molecule binding; gamma-catenin binding; glycoprotein binding; GTPase activating protein binding; protein binding

Biological Process: apoptosis; cell structure disassembly during apoptosis; cell-cell adhesion; extracellular matrix disassembly; extracellular matrix organization and biogenesis; homophilic cell adhesion; intercellular junction assembly and maintenance; negative regulation of cell-cell adhesion; neurite development; pituitary gland development; positive regulation of transcription factor import into nucleus; positive regulation of transcription, DNA-dependent; programmed cell death; regulation of immune response; response to drug; response to toxin; small GTPase mediated signal transduction; synaptogenesis

Disease: Breast Cancer; Endometrial Cancer; Gastric Cancer, Hereditary Diffuse; Ovarian Cancer; Prostate Cancer