Rabbit anti-Human IL33 Polyclonal Antibody | anti-IL33 antibody

Rabbit Polyclonal (IgG) to Human IL33

Cat. Num. AAA242172

• Gene Names: IL33; DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: IL33; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human IL33; C9orf26; DVS27; DVS27-related protein; IL-1F11; IL-33; Interleukin-33; NFEHEV; NF-HEV; Interleukin-1 family member 11; NFHEV; IL1F11; Interleukin 33; RP11-575C20.2; anti-IL33 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: a 19 amino acid peptide from near the center of human IL-33
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 270

• Applicable Applications for anti-IL33 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), Western Blot (1 ug/ml); Other dilution to be determined by researcher. ; ; Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antbibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml.
• Immunogen: IL-33 antibody was raised against synthetic peptide from human IL33 / IL-33.
• Antigen Type: Synthetic peptide
• Immunogen: IL-33 antibody was raised against synthetic peptide from human IL33 / IL-33
• Epitope: Internal
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-IL-33 antibody IHC of human uterus, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of IL-33 in Human Lymph Node cells with IL-33 antibody at 20 ug/ml.)

Western Blot (WB)

(Western blot of IL-33 in human lymph node tissue lysate with IL-33 antibody at (A) 1 and (B) 2 ug/ml.)

Related Product Information for anti-IL33 antibody

Interleukin-33 (IL33) is a cytokine belonging to the IL-1 superfamily. IL33 induces helper T cells, mast cells, eosinophils and basophils to produce type 2 cytokines. IL33 mediates its biological effects by interacting with the receptors ST2 (aka IL1RL1) and IL1 Receptor Accessory Protein (IL1RAP), activating intracellular molecules in the NF-kappaB and MAP kinase signaling pathways that drive production of type 2 cytokines (e.g. IL5 and IL13) from polarized Th2 cells.

Product Categories/Family for anti-IL33 antibody

Family: Interleukin

NCBI and Uniprot Product Information

• NCBI GI #: 15559209
• NCBI GeneID: 90865
• NCBI Accession #: NP_254274.1
• NCBI GenBank Nucleotide #: NM_033439.3
• UniProt Accession #: O95760; Q2YEJ5; B2R8L1; B4DJ35; B4E1Q9; D3DRI5; E7EAX4
• Molecular Weight: 16,250 Da
• NCBI Official Full Name: interleukin-33 isoform 1
• NCBI Official Synonym Full Names: interleukin 33
• NCBI Official Symbol: IL33
• NCBI Official Synonym Symbols: DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26
• NCBI Protein Information: interleukin-33; IL-33; IL-1F11; DVS27-related protein; interleukin-1 family member 11; nuclear factor for high endothelial venules; nuclear factor from high endothelial venules
• UniProt Protein Name: Interleukin-33
• Protein Family: Interleukin
• UniProt Gene Name: IL33
• UniProt Synonym Gene Names: C9orf26; IL1F11; NFHEV; IL-33; IL-1F11; NF-HEV
• UniProt Entry Name: IL33_HUMAN

NCBI Description

IL33 (MIM 608678) is a member of the IL1 (see MIM 147760) family that potently drives production of T helper-2 (Th2)-associated cytokines (e.g., IL4; MIM 147780). IL33 is a ligand for IL33R (IL1RL1; MIM 601203), an IL1 family receptor that is selectively expressed on Th2 cells and mast cells (summary by Yagami et al., 2010 [PubMed 20926795]).[supplied by OMIM, Jan 2011]

Uniprot Description

IL33: Cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Induces T-helper type 2-associated cytokines. Belongs to the IL-1 family. Highly divergent. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 9p24.1

Cellular Component: extracellular space; transport vesicle; chromosome; nucleus

Molecular Function: protein binding; cytokine activity

Biological Process: negative regulation of T-helper 1 type immune response; T-helper 2 type immune response; positive regulation of T-helper 2 type immune response; transcription, DNA-dependent; positive regulation of interleukin-6 production; positive regulation of immunoglobulin secretion; negative regulation of leukocyte migration; positive regulation of interleukin-4 production; negative regulation of immunoglobulin secretion; negative regulation of interferon-gamma production; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-13 production; positive regulation of interleukin-5 production; positive regulation of inflammatory response; positive regulation of macrophage activation