Western Blot (WB)
(All lanes : Anti-SLC16A10 Antibody (C-term) at 1:2000 dilutionLane 1: human skeletal muscle lysatesLane 2: CCRF-CEM whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 55 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human SLC16A10 Polyclonal Antibody | anti-SLC16A10 antibody
SLC16A10 Antibody (C-term)
FC~~1:10~50
WB~~1:1000
Western Blot (WB)
(All lanes : Anti-SLC16A10 Antibody (C-term) at 1:2000 dilutionLane 1: human skeletal muscle lysatesLane 2: CCRF-CEM whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 55 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-SLC16A10 Antibody (C-term) at 1:2000 dilutionLane 1: human skeletal muscle lysatesLane 2: CCRF-CEM whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 55 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(SLC16A10 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SLC16A10 antibody detected the SLC16A10 protein (arrow).)
Western Blot (WB)
(SLC16A10 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SLC16A10 antibody detected the SLC16A10 protein (arrow).)
Immunohistochemistry (IHC)
(SLC16A10 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SLC16A10 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(SLC16A10 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SLC16A10 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(SLC16A10 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(SLC16A10 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Testing Data (TD)
(MBS9205038 staining SLC16A10 in Humanskeletal muscle tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embeddedsections). Tissue was fixed with formaldehydeand blocked with 3% BSA for 0. 5 hour at roomtemperature; antigen retrieval was by heatmediation with a citrate buffer (pH6). Sampleswere incubated with primary antibody (1/25)for 1 hours at 37°C. A undiluted biotinylatedgoat polyvalent antibody was used as thesecondary antibody)
Testing Data (TD)
(MBS9205038 staining SLC16A10 in Humanskeletal muscle tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embeddedsections). Tissue was fixed with formaldehydeand blocked with 3% BSA for 0. 5 hour at roomtemperature; antigen retrieval was by heatmediation with a citrate buffer (pH6). Sampleswere incubated with primary antibody (1/25)for 1 hours at 37°C. A undiluted biotinylatedgoat polyvalent antibody was used as thesecondary antibody)
acid transporters that mediate the Na(+)-independent transport of
aromatic amino acids across the plasma membrane.
Friesema, E.C., et al. Mol. Endocrinol. 22(6):1357-1369(2008)
Broer, S. Physiol. Rev. 88(1):249-286(2008)
Park, S.Y., et al. Arch. Pharm. Res. 28(4):421-432(2005)
Halestrap, A.P., et al. Pflugers Arch. 447(5):619-628(2004)
NCBI and Uniprot Product Information
NCBI Description
SLC16A10 is a member of a family of plasma membrane amino acid transporters that mediate the Na(+)-independent transport of aromatic amino acids across the plasma membrane.[supplied by OMIM, Apr 2004]
Uniprot Description
SLC16A10: Sodium-independent transporter that mediates the update of aromatic acid. Can function as a net efflux pathway for aromatic amino acids in the basosolateral epithelial cells. Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.
Protein type: Transporter; Membrane protein, integral; Transporter, SLC family; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 6q21-q22
Cellular Component: basolateral plasma membrane; integral to membrane; plasma membrane
Molecular Function: transporter activity
Biological Process: amino acid transport; aromatic amino acid transport; ion transport; transmembrane transport
Research Articles on SLC16A10
Similar Products
Product Notes
The SLC16A10 slc16a10 (Catalog #AAA9205038) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 301-329. The SLC16A10 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SLC16A10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). IHC-P~~1:10~50FC~~1:10~50
WB~~1:1000. Researchers should empirically determine the suitability of the SLC16A10 slc16a10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SLC16A10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
