Western Blot (WB)
(Western blot analysis of TPPP using HeLa whole lysates.)
Rabbit anti-Human TPPP Polyclonal Antibody | anti-TPPP antibody
TPPP Antibody
Gene Names
TPPP; p24; p25; TPPP1; TPPP/p25; p25alpha
Reactivity
Human
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
TPPP; Polyclonal Antibody; TPPP Antibody; 25 kDa brain specific protein; 25 kDa brain-specific protein; Brain specific protein p25 alpha; Glycogen synthase kinase 3 (GSK3) inhibitor p24; OTTHUMP00000161630; p24; p25; p25-alpha; p25alpha; TPPP/p25; TPPP_HUMAN; TPPP1; Tubulin polymerization promoting protein; Tubulin polymerization-promoting protein; anti-TPPP antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
TPPP antibody detects endogenous levels of total TPPP
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
219
Applicable Applications for anti-TPPP antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human TPPP
Subcellular Location
Cytoplasm. Cytoplasm, Cytoskeleton. Nucleus. Localizes to glial Lewy bodies in the brains of individuals with synucleinopathies.
Tissue Specificity
Widely expressed.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of TPPP using HeLa whole lysates.)
Western Blot (WB)
(Western blot analysis of TPPP using HeLa whole lysates.)
Related Product Information for anti-TPPP antibody
Function: May play a role in the polymerization of tubulin into microtubules, microtubule bundling and the stabilization of existing microtubules, thus maintaining the integrity of the microtubule network. May play a role in mitotic spindle assembly and nuclear envelope breakdown.
Subunit Structure: Homodimer. Binds tubulin; binding is inhibited by GTP (By similarity). Interacts with GSK3 (By similarity). Interacts with MAPK1 (By similarity). Interacts with GAPDH; the interaction is direct (By similarity). Interacts with LIMK1 (via the PDZ domain); the interaction is direct.
Post-translational Modifications: Poor substrate for GSK3 (By similarity). Phosphorylated by LIMK1 on serine residues. Phosphorylation may alter the tubulin polymerization activity.
Similarity: Belongs to the TPPP family.
Subunit Structure: Homodimer. Binds tubulin; binding is inhibited by GTP (By similarity). Interacts with GSK3 (By similarity). Interacts with MAPK1 (By similarity). Interacts with GAPDH; the interaction is direct (By similarity). Interacts with LIMK1 (via the PDZ domain); the interaction is direct.
Post-translational Modifications: Poor substrate for GSK3 (By similarity). Phosphorylated by LIMK1 on serine residues. Phosphorylation may alter the tubulin polymerization activity.
Similarity: Belongs to the TPPP family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 24 kDa
Predicted: 24 kDa
Predicted: 24 kDa
NCBI Official Full Name
tubulin polymerization-promoting protein
NCBI Official Synonym Full Names
tubulin polymerization promoting protein
NCBI Official Symbol
TPPP
NCBI Official Synonym Symbols
p24; p25; TPPP1; TPPP/p25; p25alpha
NCBI Protein Information
tubulin polymerization-promoting protein
UniProt Protein Name
Tubulin polymerization-promoting protein
Protein Family
UniProt Gene Name
TPPP
UniProt Synonym Gene Names
TPPP1; TPPP
Uniprot Description
May play a role in the polymerization of tubulin into microtubules, microtubule bundling and the stabilization of existing microtubules, thus maintaining the integrity of the microtubule network. May play a role in mitotic spindle assembly and nuclear envelope breakdown.
Research Articles on TPPP
Similar Products
Product Notes
The TPPP tppp (Catalog #AAA9608416) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TPPP Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TPPP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the TPPP tppp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TPPP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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