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Clone
1983 results for "FC Blocking" - showing 1900-1950
Immunofluorescence (IF)
(Immunofluorescence staining of fixed mouse splenocytes with anti-PD-1H antibody MH5A (MBS488594) Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of MH5A () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.)
PD-1H, Monoclonal Antibody (Cat# AAA488595)
Full Name
Anti-PD-1H [MH5A]
Gene Names
Vsir; Dies1; PD-1H; VISTA; 4632428N05Rik
Reactivity
Mouse
Applications
EIA, IHC, BL
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed RAW264.7 cells with anti-CD153 antibody RM153 (MBS488626) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of RG9-35 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
CD153, Monoclonal Antibody (Cat# AAA488626)
Full Name
Anti-CD153 [RM153]
Gene Names
Tnfsf8; CD153; Cd30l; CD30LG
Reactivity
Mouse
Applications
FC/FACS, BL, FA
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
ELISA (EIA)
(ELISA of anti-CTLA-4 antibody on mouse CTLA-4-Fc fusion protein. Binding curves of the rabbit chimeric version of the anti-CTLA-4 antibody 9H10 (; red line) and isotype control (anti-Fluorescein antibody MBS488041 4-4-20 (enhanced); blue line) to an ELISA plate coated with mouse CTLA-4-Fc fusion protein (Pr00155-1.9) at a concentration of 5ug/ml. A 3-fold serial dilution from 10,000 to 0.17 ng/ml was performed using . For signal detection, a 1:4000 dilution of HRP-labelled anti-rabbit IgG1 antibody was used.)
CTLA-4, Monoclonal Antibody (Cat# AAA488590)
Full Name
Anti-CTLA-4 [9H10]
Gene Names
Ctla4; Cd152; Ly-56; Ctla-4
Reactivity
Mouse
Applications
WB, FC/FACS, EIA
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of RAW 264.7 cells using anti-Tim-4 antibody (MBS488622) 3A1. Immunofluorescence analysis of paraformaldehyde fixed RAW 264.7 cells stained with the chimeric rabbit IgG version of 3A1 (MBS488622) at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488622, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (MBS488041) followed by Alexa Fluor 488 secondary antibody.)
Tim-4, Monoclonal Antibody (Cat# AAA488623)
Full Name
Anti-Tim-4 [3A1]
Gene Names
Timd4; Tim4; TIM-4; B430010N18Rik
Reactivity
Mouse
Applications
FC/FACS, WB, EIA, IHC, IF, BL
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Western Blot using anti-HVEM antibody HMHV-1B18 (MBS488601) Mouse spleen(A), mouse thymus (B) and mouse lung(C) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HMHV-1B18 (MBS488601) at 0.01ug/ml, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
HVEM, Monoclonal Antibody (Cat# AAA488600)
Full Name
Anti-HVEM [HMHV-1B18]
Reactivity
Mouse
Applications
FC/FACS, WB, IP, BL, FA
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed RAW264.7 cells with anti-CD80 antibody RM80 (B467) (MBS488602) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of RM80 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
CD80, Monoclonal Antibody (Cat# AAA488602)
Full Name
Anti-CD80 [RM80]
Gene Names
Cd80; B71; Ly53; TSA1; Cd28l; Ly-53; MIC17
Reactivity
Mouse
Applications
FC/FACS, WB, IHC, BL, FA
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed RAW264.7 cells with anti-CD86 antibody PO.3 (B467) (MBS488604) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of PO.3 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
CD86, Monoclonal Antibody (Cat# AAA488604)
Full Name
Anti-CD86 [PO.3]
Gene Names
CD86; B70; B7-2; B7.2; LAB72; CD28LG2
Reactivity
Mouse
Applications
FC/FACS, WB, IHC, BL, FA
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Western Blot using anti-Podoplanin (MAP tag) antibody PMab-1 () Mouse thymus (A), mouse brain (B), NIH3T3 cells (C) and mouse kidney (D) lysates (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of 1B4 (MBS488486) at 0.01ug/ml, 0.1ug/ml, 0.001ug/ml and 0.003ug/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Podoplanin, Monoclonal Antibody (Cat# AAA488581)
Full Name
Anti-Podoplanin (MAP tag) [PMab-1]
Gene Names
Pdpn; T1a; Gp38; OTS-8; T1alpha; RANDAM-2; T1-alpha
Reactivity
Mouse
Applications
EIA, WB, IHC, BL
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
C5a Receptor, Monoclonal Antibody (Cat# AAA524535)
Full Name
Anti-Mouse C5a Receptor (CD88), FITC (clone 20/70)(rat IgG2b)
Reactivity
Mouse
Applications
FC/FACS, IF, BL
Pricing
$290/0.1 mg | $1,090/5x0.1 mg
Immunocytochemistry (ICC)
(Purified Anti-Mouse CD4 (CL012AP) staining of paraformaldehyde-fixed paraffin-embedded spleen sections at 10x (left) and 100x (right) magnification. (Rat anti-mouse CD4 mAb (CL012AP) 1:500; Alexa Fluor 488 Goat anti-rat 1:100 (Molecular Probes) and Normal Goat Serum (blocking)))
CD72.1, Monoclonal Antibody (Cat# AAA524584)
Full Name
Anti-Mouse CD72.1, Purified (Clone CT-72.1)(mouse IgG2a)
Reactivity
Mouse
Applications
FC/FACS
Purity
Purified
Pricing
$395/0.2 mg | $1,555/5x0.2 mg
Flow Cytometry (FC/FACS)
(Human peripheral blood lymphocytes were stained with anti-HLA-ABC (clone: W6/32)(filled histogram) or Mouse IgG2a isotype control (open histogram). )
C5a Receptor, Monoclonal Antibody (Cat# AAA524533)
Full Name
Anti-Mouse C5a Receptor (CD88), Purified (clone 20/70)(rat IgG2b)
Reactivity
Mouse
Applications
FC/FACS, IF, BL
Purity
Purified. Protein G Chromatography
Pricing
$370/0.25 mg | $1,450/5x0.25 mg
CD54, Monoclonal Antibody (Cat# AAA1801845)
Full Name
Anti-Ms CD54 Purified Low Endotoxin
Gene Names
Icam1; CD54; Ly-47; Icam-1; MALA-2
Reactivity
Mouse
Applications
FC/FACS, QC tested, IP, WB, IHC-F, ICC, FUNC
Purity
Purified by protein-G affinity chromatography
Immunohistochemistry (IHC)-Paraffin
( Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (Biliverdin) Polyclonal Antibody, Unconjugated (bs-12868R) at 1:500 overnight at 4 degree C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. )
Biliverdin Reductase, Polyclonal Antibody (Cat# AAA9234579)
Full Name
Biliverdin Reductase Polyclonal Antibody
Gene Names
BLVRA; BVR; BLVR; BVRA
Reactivity
Rat, Pig
Applications
IHC-P, IHC-F, IF, ICC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Immunohistochemistry (IHC)-Paraffin
( Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (FAM98A) Polyclonal Antibody, Unconjugated (bs-11012R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
FAM98A, Polyclonal Antibody (Cat# AAA9233913)
Full Name
FAM98A Polyclonal Antibody
Reactivity
Rat, Pig, Cow
Applications
IHC-P, IHC-F, IF, ICC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Flow Cytometry (FC/FACS)
(Flow-cytometry using the Anti-IL-9 antibody D9302C12 (MBS488651). Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of D9302C12 (MBS488651, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.)
IL-9, Monoclonal Antibody (Cat# AAA488650)
Full Name
Anti-IL-9 [D9302C12]
Gene Names
Il9; P40; Il-9
Reactivity
Mouse
Applications
BL, FC/FACS
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunohistochemistry (IHC)-Paraffin
(Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min; Incubation: Anti-Gemin 7 Polyclonal Antibody, Unconjugated(bs-13330R) 1:200, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining )
Gemin 7, Polyclonal Antibody (Cat# AAA9234708)
Full Name
Gemin 7 Polyclonal Antibody
Gene Names
GEMIN7; SIP3
Reactivity
Rat, Dog, Cow
Applications
IHC-P, IHC-F, IF, ICC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Immunohistochemistry (IHC)-Paraffin
( Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (Gbx1) Polyclonal Antibody, Unconjugated (bs-11660R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
Gbx1, Polyclonal Antibody (Cat# AAA9234159)
Full Name
Gbx1 Polyclonal Antibody
Gene Names
GBX1; Huh-17
Reactivity
Rat, Pig, Chimpanzee, Dog
Applications
IHC-P, IHC-F, IF, ICC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Immunofluorescence (IF)
(Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min; Incubation: Anti-DYNC1I1 Polyclonal Antibody, FITC conjugated(bs-10470R-FITC) 1:200, 60 minutes at 37 degree C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei )
DYNC1I1, Polyclonal Antibody (Cat# AAA9233842)
Full Name
DYNC1I1 Polyclonal Antibody
Gene Names
DYNC1I1; DNCI1; DNCIC1
Reactivity
Rat, Pig, Cow
Applications
WB, IHC-P, IHC-F, IF, ICC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Immunohistochemistry (IHC)-Paraffin
( Paraformaldehyde-fixed, paraffin embedded (Rat small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (BTBD7) Polyclonal Antibody, Unconjugated (bs-7979R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
BTBD7, Polyclonal Antibody (Cat# AAA9238393)
Full Name
BTBD7 Polyclonal Antibody
Gene Names
BTBD7; FUP1
Reactivity
Rat, Pig, Dog, Cow
Applications
IHC-P, IHC-F, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Flow Cytometry (FC/FACS)
(Flow-cytometry using the Anti-IL-9 antibody D9302C12 (MBS488651). Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of D9302C12 (, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.)
IL-9, Monoclonal Antibody (Cat# AAA488651)
Full Name
Anti-IL-9 [D9302C12]
Gene Names
Il9; P40; Il-9
Reactivity
Mouse
Applications
BL, FC/FACS
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
ELISA (EIA)
(ELISA of anti-CD96 antibody on CD96-Fc fusion protein. Binding curve of the rabbit chimeric version of the anti-CD96 antibody 6A6 (MBS488664; blue line) to an ELISA plate coated with mouse CD96-Fc fusion protein (Pr00230-1.9) at a concentration of 5ug/ml. A 3-fold serial dilution from 10000 to 1.7 ng/ml was performed using MBS488664. For signal detection, a 1:4000 dilution of anti-rabbit IgG1 HRP (BioRad) antibody was used.)
CD96, Monoclonal Antibody (Cat# AAA488665)
Full Name
Anti-CD96 [6A6]
Gene Names
Cd96; Tactile; 1700109I12Rik
Reactivity
Mouse
Applications
BL, FC/FACS
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
(Blank control: Raw264.7. Primary Antibody (green line): Rabbit Anti-CCR-2 antibody (bs-23026R) Dilution: 1 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1 µg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. )
CCR-2, Polyclonal Antibody (Cat# AAA9237559)
Full Name
CCR-2 Polyclonal Antibody
Gene Names
Ccr2; Ckr2; Ccr2a; Ccr2b; Ckr2a; Ckr2b; mJe-r; Cmkbr2; Cc-ckr-2
Reactivity
Rat
Applications
WB, IHC-P, IHC-F, IF, ICC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Immunofluorescence (IF)
(Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min; Incubation: Anti-APH1a Polyclonal Antibody, Unconjugated(bs-4259R) 1:200, overnight at 4 degree C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37 degree C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei)
APH1a, Polyclonal Antibody (Cat# AAA9237711)
Full Name
APH1a Polyclonal Antibody
Gene Names
APH1A; APH-1; APH-1A; CGI-78; 6530402N02Rik
Reactivity
Rat, Pig, Dog, Cow
Applications
WB, IHC-P, IHC-F, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Immunohistochemistry (IHC)-Paraffin
(Tissue/cell: Mouse embryos; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min; Incubation:Anti-CDCA5/Sororin Polyclonal Antibody, Unconjugated(bs-7717R) 1:200, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining )
CDCA5, Polyclonal Antibody (Cat# AAA9238301)
Full Name
CDCA5 Polyclonal Antibody
Gene Names
CDCA5; SORORIN
Reactivity
Rat, Pig, Dog, Cow
Applications
IHC-P, IHC-F, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Flow Cytometry (FC/FACS)
(Black line : Positive blank control (Hela); Negative blank control (A431) Green line : Primary Antibody (Rabbit Anti-CLEC 4E antibody (bs-8541R) ) Orange line?Isotype Control Antibody (Rabbit IgG) . Blue line : Secondary Antibody (Goat anti-rabbit IgG-PE)/(Goat anti-rabbit IgG-AF488) Hela(Positive)and A431(Negative control)cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CLEC 4E Antibody(bs-8541R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange). )
CLEC 4E, Polyclonal Antibody (Cat# AAA9238603)
Full Name
CLEC 4E Polyclonal Antibody
Gene Names
CLEC4E; MINCLE; CLECSF9
Reactivity
Rat, Cow
Applications
IHC-P, IHC-F, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Flow Cytometry (FC/FACS)
(U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Galectin 12 Antibody(bs-8413R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).)
Galectin 12, Polyclonal Antibody (Cat# AAA9238569)
Full Name
Galectin 12 Polyclonal Antibody
Gene Names
LGALS12; GAL12; GRIP1
Reactivity
Rat
Applications
WB, IHC-P, IHC-F, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Immunohistochemistry (IHC)-Paraffin
(Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min; Incubation: Anti-Transglutaminase Polyclonal Antibody, Unconjugated(bs-6690R) 1:200, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining )
Transglutaminase-1, Polyclonal Antibody (Cat# AAA9238064)
Full Name
Transglutaminase-1 Polyclonal Antibody
Gene Names
TGM1; LI; KTG; LI1; TGK; ICR2; ARCI1; TGASE
Reactivity
Rat, Pig, Dog
Applications
IHC-P, IHC-F, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
ELISA (EIA)
(Binding curve of anti-VISTA antibody 13F3 (MBS488691) to recombinant mouse VISTA Fc-Fusion Protein. ELISA Plate coated with recombinant mouse VISTA Fc-Fusion Protein at a concentration of 5ug/ml. A 3-fold serial dilution from 10,000 ng/ml was performed using MBS488691. For detection, a 1:4000 dilution of HRP-labelled anti-rabbit antibody was used.)
VISTA, Monoclonal Antibody (Cat# AAA488690)
Full Name
Anti-VISTA [13F3]
Gene Names
Vsir; Dies1; PD-1H; VISTA; 4632428N05Rik
Reactivity
Mouse
Applications
EIA, FC/FACS, BL
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
(Blank control(blue): TM4 cells(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody:Rabbit Anti-PIWIL1 antibody(bs-20224R), Dilution: 5 µg in 100 µL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.)
PIWIL1, Polyclonal Antibody (Cat# AAA9237351)
Full Name
PIWIL1 Polyclonal Antibody
Gene Names
PIWIL1; HIWI; MIWI; PIWI
Reactivity
Pig, Dog, Cow
Applications
IHC-P, IHC-F, IF, ICC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Flow Cytometry (FC/FACS)
(Blank control (Black line): Molt4 (Black). Primary Antibody (green line):Rabbit Anti-HSD17B6 antibody (bs-6592R) Dilution:3 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 3 µg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. )
HSD17B6, Polyclonal Antibody (Cat# AAA9238050)
Full Name
HSD17B6 Polyclonal Antibody
Gene Names
HSD17B6; HSE; RODH; SDR9C6
Reactivity
Rat, Pig
Applications
WB, IHC-P, IHC-F, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Flow Cytometry (FC/FACS)
(Blank control: Hela(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.. Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1 µg in 100 µL1X PBS containing 0.5% BSA(green). )
HDPTP, Polyclonal Antibody (Cat# AAA9235563)
Full Name
HDPTP Polyclonal Antibody
Gene Names
PTPN23; HDPTP; HD-PTP; PTP-TD14
Reactivity
Rat, Pig
Applications
IHC-P, IHC-F, IF, ICC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Immunohistochemistry (IHC)-Paraffin
(Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min; Incubation: Anti-HELZ Polyclonal Antibody, Unconjugated(bs-15450R) 1:500, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining )
HELZ, Polyclonal Antibody (Cat# AAA9235574)
Full Name
HELZ Polyclonal Antibody
Gene Names
HELZ; DHRC; DRHC; HUMORF5
Reactivity
Rat
Applications
IHC-P, IHC-F, IF, ICC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Immunohistochemistry (IHC)-Paraffin
(Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (FMNL3) Polyclonal Antibody, Unconjugated (bs-16151R) at 1:200 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. )
FMNL3, Polyclonal Antibody (Cat# AAA9235724)
Full Name
FMNL3 Polyclonal Antibody
Gene Names
FMNL3; FRL2; WBP3; FHOD3; WBP-3
Reactivity
Rat, Dog, Cow
Applications
WB, IHC-P, IHC-F, IF, ICC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Immunocytochemistry (ICC)
(Tissue/cell: human glioma cells(U251 cells);4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min; Incubation: Anti-CASPR Polyclonal Antibody, Unconjugated(bs-11128R) 1:200, overnight at 4 degree C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37 degree C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei )
CASPR, Polyclonal Antibody (Cat# AAA9233964)
Full Name
CASPR Polyclonal Antibody
Gene Names
CNTNAP1; P190; CASPR; NRXN4; CNTNAP
Reactivity
Rat, Pig, Dog, Cow
Applications
WB, IHC-P, IHC-F, IF, ICC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Flow Cytometry (FC/FACS)
(Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-SLC25A18 antibody (bs-21221R) Dilution: 1 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 1 µg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST methanol for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. )
SLC25A18, Polyclonal Antibody (Cat# AAA9237503)
Full Name
SLC25A18 Polyclonal Antibody
Reactivity
Rat
Applications
WB, IHC-P, IHC-F, IF, ICC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Immunohistochemistry (IHC)-Paraffin
(Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min; Incubation: Anti-CLEC5A/MDL1 Polyclonal Antibody, Unconjugated(bs-2663R) 1:200, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining )
CLEC5A, Polyclonal Antibody (Cat# AAA9237618)
Full Name
CLEC5A Polyclonal Antibody
Gene Names
Clec5a; Mdl1; Ly100; MDL-1; Clecsf5
Reactivity
Rat, Pig, Cow
Applications
WB, IHC-P, IHC-F, IF, ICC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Immunohistochemistry (IHC)-Paraffin
( Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (5HT3B receptor) Polyclonal Antibody, Unconjugated (bs-4289R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
5HT3B receptor, Polyclonal Antibody (Cat# AAA9237721)
Full Name
5HT3B receptor Polyclonal Antibody
Gene Names
HTR3B; 5-HT3B
Reactivity
Rat, Cow
Applications
WB, IHC-P, IHC-F, IF, ICC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Flow Cytometry (FC/FACS)
(Blank control: RSC96(blue) Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-AF647(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1 µl in 100 µL1X PBS containing 0.5% BSA(green). )
NARG2, Polyclonal Antibody (Cat# AAA9237925)
Full Name
NARG2 Polyclonal Antibody
Gene Names
NARG2; BRCC1
Reactivity
Rat, Dog
Applications
WB, IHC-P, IHC-F, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Immunohistochemistry (IHC)-Paraffin
(Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (CAF1p55/VPRBP) Polyclonal Antibody, Unconjugated (bs-9393R) at 1:200 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
CAF1p55/VPRBP, Polyclonal Antibody (Cat# AAA9238815)
Full Name
CAF1p55/VPRBP Polyclonal Antibody
Gene Names
DCAF1; RIP; VPRBP
Reactivity
Rat
Applications
WB, IHC-P, IHC-F, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed human peripheral blood leukocytes with anti-CD106 antibody B-K9 (C106-K9) (MBS488749) Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leukocytes on Shi-fix coverslips stained with the chimeric rabbit IgG version of B-K9 (C106-K9) () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
CD106, Monoclonal Antibody (Cat# AAA488749)
Full Name
Anti-CD106 [B-K9 (C106-K9)]
Gene Names
VCAM1; CD106; INCAM-100
Reactivity
Human
Applications
BL, FC/FACS
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Wester Blot (WB)
(Dilution: WB 1:2000All lanes : Anti-PSA Antibody (Center) at 1:2000 dilutionLane 1: mouse brain tissue lysateLysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG/A/M(H/L), Peroxidase conjugated at 1/2000 dilution. Observed band size : 110kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
PSA, Polyclonal Antibody (Cat# AAA9241898)
Full Name
PSA Antibody (Center)
Gene Names
NPEPPS; PSA; AAP-S; MP100
Reactivity
Human
Predicted Reactivity: Mouse
Predicted Reactivity: Mouse
Applications
WB, IHC-P, FC/FACS
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Flow Cytometry (FC/FACS)
(Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-PINX-1 antibody (bs-9594R) Dilution: 2 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1 µg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20 degree C. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed )
PINX-1, Polyclonal Antibody (Cat# AAA9238872)
Full Name
PINX-1 Polyclonal Antibody
Gene Names
PINX1; LPTL; LPTS
Reactivity
Rat, Pig, Cow
Applications
WB, IHC-P, IHC-F, IF, ICC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Immunohistochemistry (IHC)-Paraffin
(Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (NEDD4) Polyclonal Antibody, Unconjugated (bs-7877R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. )
NEDD4, Polyclonal Antibody (Cat# AAA9238360)
Full Name
NEDD4 Polyclonal Antibody
Gene Names
NEDD4; RPF1; NEDD4-1
Reactivity
Rat, Pig, Dog, Cow
Applications
WB, IHC-P, IHC-F, IF, ICC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Immunohistochemistry (IHC)-Paraffin
(Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (Dopamine Receptor D1) Polyclonal Antibody, Unconjugated (bs-10612R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
Dopamine Receptor D1, Polyclonal Antibody (Cat# AAA9233865)
Full Name
Dopamine Receptor D1 Polyclonal Antibody
Gene Names
Drd1a; Drd1; Drd-1; Gpcr15; C030036C15Rik
Reactivity
Rat
Applications
WB, IHC-P, IHC-F, IF, ICC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Flow Cytometry (FC/FACS)
(Blank control: Hela. Primary Antibody (green line): Rabbit Anti-ABCA7 antibody (bs-11180R) Dilution: 1 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1 µg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min atroom temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. )
ABCA7, Polyclonal Antibody (Cat# AAA9233989)
Full Name
ABCA7 Polyclonal Antibody
Gene Names
ABCA7; ABCX; ABCA-SSN
Reactivity
Rat
Applications
IHC-P, IHC-F, IF, ICC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Western Blot (WB)
(Western blot of human A431 cells treated with Calyculin A (100nM) for 30 min. Blot lanes were untreated (lanes 1, 3, & 5) or treated with lambda phosphatase (lanes 2, 4, & 6) then probed with anti-ATM (Ser-794) (lanes 1 & 2), anti-ATM (C-Terminal) (lanes 3 & 4), or anti-ATM (Ser-1981) (lanes 5 & 6).)
ATM, Polyclonal Antibody (Cat# AAA474186)
Full Name
ATM (Ser-794), phospho-specific Antibody
Gene Names
ATM; AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1
Reactivity
Human, Rat, Mouse
Applications
WB, EIA, ICC, FC/FACS
Pricing
$415/0.1 mL | $1,720/5x0.1 mL
Immunofluorescence (IF)
(Immunofluorescence staining of fixed U937 cells with anti-C5aR antibody S5/1 (MBS488755) Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version S5/1 (MBS488755) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488755, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
C5aR, Monoclonal Antibody (Cat# AAA488754)
Full Name
Anti-C5aR [S5/1]
Gene Names
C5AR1; C5A; C5AR; C5R1; CD88
Reactivity
Human, Rabbit, Cow, Ferret
Applications
BL, WB, FC/FACS, IP, EIA
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
(Blank control: K562. Primary Antibody (green line): Rabbit Anti-UBE2V1 antibody (bs-7882R) Dilution: 2 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1 µg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20 degree C. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. )
UBE2V1, Polyclonal Antibody (Cat# AAA9238362)
Full Name
UBE2V1 Polyclonal Antibody
Gene Names
UBE2V1; CIR1; UEV1; CROC1; UBE2V; UEV-1; UEV1A; CROC-1
Reactivity
Rat, Pig
Applications
IHC-P, IHC-F, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Immunohistochemistry (IHC)-Paraffin
(Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min; Incubation: Anti-FOXRED2 Polyclonal Antibody, Unconjugated(bs-13210R) 1:200, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining )
FOXRED2, Polyclonal Antibody (Cat# AAA9234664)
Full Name
FOXRED2 Polyclonal Antibody
Gene Names
FOXRED2; ERFAD
Reactivity
Rat, Pig, Cow
Applications
WB, IHC-P, IHC-F, IF, ICC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Flow Cytometry (FC/FACS)
(Blank control: Hela(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1 µg in 100 µL1X PBS containing 0.5% BSA(green). )
Giantin, Polyclonal Antibody (Cat# AAA9234717)
Full Name
Giantin Polyclonal Antibody
Gene Names
GOLGB1; GCP; GCP372; GOLIM1
Reactivity
Rat, Dog
Applications
IHC-P, IHC-F, IF, ICC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
