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Reactivity
Gene Name
Isotype
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Application
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1983 results for "FC Blocking" - showing 1950-1983
Flow Cytometry (FC/FACS)
(Blank control:Mouse spleen. Primary Antibody (green line): Rabbit Anti-TIFA antibody (bs-13669R) Dilution: 2 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1 µg /test. Protocol The cells were fixed with 70% ethanol (10min at room temperature) and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. )
TIFA, Polyclonal Antibody (Cat# AAA9234810)
Full Name
TIFA Polyclonal Antibody
Gene Names
TIFA; T2BP; T6BP; TIFAA
Reactivity
Rat, Pig, Dog, Cow
Applications
IHC-P, IHC-F, IF, ICC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Immunohistochemistry (IHC)-Paraffin
(Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (TRIM32) Polyclonal Antibody, Unconjugated (bs-0964R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
TRIM32, Polyclonal Antibody (Cat# AAA9233792)
Full Name
TRIM32 Polyclonal Antibody
Gene Names
Trim32; 3f3; BBS11; Zfp117; 1810045E12Rik
Reactivity
Rat
Applications
WB, IHC-P, IHC-F, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Flow Cytometry (FC/FACS)
(Blank control:U-937. Primary Antibody (green line): Rabbit Anti-SNRNP200 antibody (bs-12657R) Dilution: 2 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 2 µg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20 degree C. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. )
SNRNP200, Polyclonal Antibody (Cat# AAA9234497)
Full Name
SNRNP200 Polyclonal Antibody
Gene Names
SNRNP200; BRR2; RP33; HELIC2; ASCC3L1; U5-200KD
Reactivity
Rat
Applications
IHC-P, IHC-F, IF, ICC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Immunofluorescence (IF)
(Immunofluorescence staining of mouse splenocytes using anti-IL-6R antibody (MBS488648) D7715A7. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of D7715A7 (MBS488648) at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488648, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (MBS488041) followed by Alexa Fluor 488 secondary antibody.)
IL-6R, Monoclonal Antibody (Cat# AAA488649)
Full Name
Anti-IL-6R [D7715A7]
Gene Names
Il6ra; Il6r; CD126; IL-6R; IL-6RA; IL-6R-alpha
Reactivity
Mouse
Applications
BL, FC/FACS
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
(Flow cytometry using the Anti-CD80 antibody 7C10 (MBS488795). Paraformaldehyde fixed Daudi cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (MBS488149; isotype control, black line) or the rabbit IgG version of 7C10 (, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
CD80, Monoclonal Antibody (Cat# AAA488795)
Full Name
Anti-CD80 [7C10]
Gene Names
CD80; B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
Reactivity
Human
Applications
BL, EIA
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Wester Blot (WB)
(Sample: testis(rat) Lysate at 40 ug Primary: Anti-IL22BP (bs-2625R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 25/28kD Observed band size: 35 kD )
IL22BP, Polyclonal Antibody (Cat# AAA9237613)
Full Name
IL22BP Polyclonal Antibody
Gene Names
IL22RA2; CRF2X; CRF2-10; CRF2-S1; IL-22BP; IL-22RA2; ZCYTOR16; IL-22R-alpha-2
Reactivity
Rat
Applications
WB, IHC-P, IHC-F, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Wester Blot (WB)
(Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti-HIF3 alpha (bs-5898R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 74 kD Observed band size: 75 kD)
HIF3 alpha, Polyclonal Antibody (Cat# AAA9237916)
Full Name
HIF3 alpha Polyclonal Antibody
Gene Names
HIF3A; IPAS; MOP7; PASD7; HIF-3A; bHLHe17
Reactivity
Rat, Pig, Dog, Cow
Applications
WB, IHC-P, IHC-F, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Immunohistochemistry (IHC)-Paraffin
(Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min; Incubation: Anti-KDM5B/PLU1/Jarid1B Polyclonal Antibody, Unconjugated(bs-6139R) 1:200, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining )
KDM5B, Polyclonal Antibody (Cat# AAA9237957)
Full Name
KDM5B Polyclonal Antibody
Gene Names
KDM5B; CT31; PLU1; PUT1; PLU-1; JARID1B; PPP1R98; RBP2-H1; RBBP2H1A
Reactivity
Rat, Pig, Dog, Cow
Applications
WB, IHC-P, IHC-F, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Immunofluorescence (IF)
(Immunofluorescence staining of mouse splenocytes using anti-CTLA-4 antibody (MBS488598) 9D9. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of 9D9 (MBS488598) at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488598, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (MBS488041) followed by Alexa Fluor 488 secondary antibody.)
CTLA-4, Monoclonal Antibody (Cat# AAA488599)
Full Name
Anti-CTLA-4 [9D9]
Gene Names
Ctla4; Cd152; Ly-56; Ctla-4
Reactivity
Mouse
Applications
BL, IF
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed U937 cells with anti-C5aR antibody S5/1 (MBS488755) Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version S5/1 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
C5aR, Monoclonal Antibody (Cat# AAA488755)
Full Name
Anti-C5aR [S5/1]
Gene Names
C5AR1; C5A; C5AR; C5R1; CD88
Reactivity
Human, Rabbit, Cow, Ferret
Applications
BL, WB, FC/FACS, IP, EIA
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
(Blank control: Hela Cells(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Phospholipase C beta 2/AF647 Conjugated antibody (bs-6472R-AF647), Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.)
Phospholipase C beta 2, Polyclonal Antibody (Cat# AAA9238023)
Full Name
Phospholipase C beta 2 Polyclonal Antibody
Reactivity
Rat, Pig, Dog, Cow
Applications
WB, IHC-P, IHC-F, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Flow Cytometry (FC/FACS)
(Blank control: A549. Primary Antibody (green line): Rabbit Anti-ENC1 antibody (bs-8043R) Dilution: 0.2 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1 µg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20 degree C. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. )
ENC1, Polyclonal Antibody (Cat# AAA9238417)
Full Name
ENC1 Polyclonal Antibody
Gene Names
ENC1; NRPB; CCL28; ENC-1; PIG10; KLHL35; KLHL37; TP53I10
Reactivity
Rat, Cow
Applications
WB, IHC-P, IHC-F, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Immunohistochemistry (IHC)-Paraffin
(Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min; Incubation: Anti-WRB Polyclonal Antibody, Unconjugated(bs-11774R) 1:200, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining )
WRB, Polyclonal Antibody (Cat# AAA9234195)
Full Name
WRB Polyclonal Antibody
Gene Names
WRB; CHD5; GET1
Reactivity
Rat, Dog, Cow
Applications
IHC-P, IHC-F, IF, ICC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Immunofluorescence (IF)
(Immunofluorescence staining of mouse splenocytes using anti-CTLA-4 antibody (MBS488598) 9D9. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of 9D9 () at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (MBS488041) followed by Alexa Fluor 488 secondary antibody.)
CTLA-4, Monoclonal Antibody (Cat# AAA488598)
Full Name
Anti-CTLA-4 [9D9]
Gene Names
Ctla4; Cd152; Ly-56; Ctla-4
Reactivity
Mouse
Applications
BL, IF
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed RAW264.7 cells with anti-CD70 antibody TAN1-6 (MBS488618) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of TAN1-6 (MBS488618) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488618, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody)
CD70, Monoclonal Antibody (Cat# AAA488619)
Full Name
Anti-CD70 [TAN1-6]
Gene Names
Cd70; Cd27l; CD27LG; Tnfsf7
Reactivity
Mouse
Applications
FC/FACS, WB, IF, BL
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of mouse splenocytes using anti-IL-6R antibody (MBS488648) D7715A7. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of D7715A7 () at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (MBS488041) followed by Alexa Fluor 488 secondary antibody.)
IL-6R, Monoclonal Antibody (Cat# AAA488648)
Full Name
Anti-IL-6R [D7715A7]
Gene Names
Il6ra; Il6r; CD126; IL-6R; IL-6RA; IL-6R-alpha
Reactivity
Mouse
Applications
BL, FC/FACS
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed K562 cells with anti-TLR2 antibody TL2.1 (MBS488705) Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of TL2.1 (MBS488705) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488705, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
TLR2, Monoclonal Antibody (Cat# AAA488704)
Full Name
Anti-TLR2 [TL2.1]
Gene Names
TLR2; TIL4; CD282
Reactivity
Human
Applications
WB, FC/FACS, IP, IF, BL
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Wester Blot (WB)
(Sample: Large intestine (Mouse) Lysate at 40 ug Primary: Anti- Selenium Binding Protein 1 (bs-4200R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD )
Selenium Binding Protein 1, Polyclonal Antibody (Cat# AAA9237703)
Full Name
Selenium Binding Protein 1 Polyclonal Antibody
Gene Names
SELENBP1; LPSB; SP56; hSBP; SBP56; HEL-S-134P
Reactivity
Rat, Dog, Cow
Applications
WB, IHC-P, IHC-F, IF, ICC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed mouse splenocytes with anti-IL2R gamma C antibody 3E12 (MBS488670) Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips stained with the chimeric rabbit IgG version of 3E12 (MBS488670) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488670, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
IL2R gamma C, Monoclonal Antibody (Cat# AAA488671)
Full Name
Anti-IL2R gamma C [3E12]
Reactivity
Mouse
Applications
FC/FACS, IP, BL
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed Daudi cells with anti-CD80 antibody 16C10 (MBS488796) Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of 16C10 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
CD80, Monoclonal Antibody (Cat# AAA488797)
Full Name
Anti-CD80 [16C10]
Gene Names
CD80; B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
Reactivity
Human
Applications
BL, EIA
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of Jurkat cells using anti-IL-9R antibody (MBS488657) AH9R2. Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of AH9R2 () at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (MBS488041) followed by Alexa Fluor 488 secondary antibody.)
IL-9R, Monoclonal Antibody (Cat# AAA488657)
Full Name
Anti-IL-9R [AH9R2]
Gene Names
IL9R; CD129; IL-9R
Reactivity
Human
Applications
IP, BL
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed RAW264.7 cells with anti-IL-10R antibody 1B1.3a (MBS488706) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of 1B1.3a () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
IL-10R, Monoclonal Antibody (Cat# AAA488706)
Full Name
Anti-IL-10R [1B1.3a]
Gene Names
Il10ra; Il10r; CDw210; CDw210a; mIL-10R; AW553859
Reactivity
Mouse
Applications
FC/FACS, BL
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed RAW264.7 cells with anti-IL-10R antibody 1B1.3a (MBS488706) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of 1B1.3a (MBS488706) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488706, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
IL-10R, Monoclonal Antibody (Cat# AAA488707)
Full Name
Anti-IL-10R [1B1.3a]
Gene Names
Il10ra; Il10r; CDw210; CDw210a; mIL-10R; AW553859
Reactivity
Mouse
Applications
FC/FACS, BL
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Wester Blot (WB)
(Sample: Cerebrum (Mouse) Lysate at 40 ug Hippocampal (Mouse) Lysate at 40 ug Primary: Anti-IL1RAPL1 (bs-0445R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78 kD Observed band size: 108 kD)
IL1RAPL1, Polyclonal Antibody (Cat# AAA9233770)
Full Name
IL1RAPL1 Polyclonal Antibody
Gene Names
IL1RAPL1; IL1R8; MRX10; MRX21; MRX34; OPHN4; IL1RAPL; TIGIRR-2
Reactivity
Rat, Pig, Dog, Cow
Applications
WB, IHC-P, IHC-F, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed RAW264.7 cells with anti-CD153 antibody RM153 (MBS488626) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of RG9-35 (MBS488626) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488626, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
CD153, Monoclonal Antibody (Cat# AAA488627)
Full Name
Anti-CD153 [RM153]
Gene Names
Tnfsf8; CD153; Cd30l; CD30LG
Reactivity
Mouse
Applications
FC/FACS, BL, FA
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed RAW264.7 cells with anti-Tim-3 antibody 2C12 (MBS488620) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of 2C12 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
Tim-3, Monoclonal Antibody (Cat# AAA488620)
Full Name
Anti-Tim-3 [2C12]
Gene Names
Havcr2; Tim3; TIM-3; Timd3
Reactivity
Mouse
Applications
FC/FACS, IF, IHC, BL
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of RAW 264.7 cells using anti-Tim-4 antibody (MBS488622) 3A1. Immunofluorescence analysis of paraformaldehyde fixed RAW 264.7 cells stained with the chimeric rabbit IgG version of 3A1 () at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (MBS488041) followed by Alexa Fluor 488 secondary antibody.)
Tim-4, Monoclonal Antibody (Cat# AAA488622)
Full Name
Anti-Tim-4 [3A1]
Gene Names
Timd4; Tim4; TIM-4; B430010N18Rik
Reactivity
Mouse
Applications
FC/FACS, WB, EIA, IHC, IF, BL
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
ELISA (EIA)
(ELISA of anti-CTLA-4 antibody on mouse CTLA-4-Fc fusion protein. Binding curves of the rabbit chimeric version of the anti-CTLA-4 antibody 9H10 (MBS488590; red line) and isotype control (anti-Fluorescein antibody MBS488041 4-4-20 (enhanced); blue line) to an ELISA plate coated with mouse CTLA-4-Fc fusion protein (Pr00155-1.9) at a concentration of 5ug/ml. A 3-fold serial dilution from 10,000 to 0.17 ng/ml was performed using MBS488590. For signal detection, a 1:4000 dilution of HRP-labelled anti-rabbit IgG1 antibody was used.)
CTLA-4, Monoclonal Antibody (Cat# AAA488589)
Full Name
Anti-CTLA-4 [9H10]
Gene Names
Ctla4; Cd152; Ly-56; Ctla-4
Reactivity
Mouse
Applications
WB, FC/FACS, EIA
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed RAW264.7 cells with anti-CD86 antibody PO.3 (B467) (MBS488604) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of PO.3 (MBS488604) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488604, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
CD86, Monoclonal Antibody (Cat# AAA488605)
Full Name
Anti-CD86 [PO.3]
Gene Names
CD86; B70; B7-2; B7.2; LAB72; CD28LG2
Reactivity
Mouse
Applications
FC/FACS, WB, IHC, BL, FA
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Western Blot using anti-HVEM antibody HMHV-1B18 (MBS488601) Mouse spleen(A), mouse thymus (B) and mouse lung(C) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HMHV-1B18 () at 0.01ug/ml, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
HVEM, Monoclonal Antibody (Cat# AAA488601)
Full Name
Anti-HVEM [HMHV-1B18]
Reactivity
Mouse
Applications
FC/FACS, WB, IP, BL, FA
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
(Flow cytometry using the Anti-CD25 antibody 2E4 (MBS488869). Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (MBS488149; isotype control, black line) or the rabbit IgG version of 2E4 (, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
CD25, Monoclonal Antibody (Cat# AAA488869)
Full Name
Anti-CD25 [2E4]
Gene Names
Il2ra; CD25; Il2r; Ly-43
Reactivity
Mouse
Applications
FC/FACS
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed RAW264.7 cells with anti-CD80 antibody RM80 (B467) (MBS488602) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of RM80 (MBS488602) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488602, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
CD80, Monoclonal Antibody (Cat# AAA488603)
Full Name
Anti-CD80 [RM80]
Gene Names
Cd80; B71; Ly53; TSA1; Cd28l; Ly-53; MIC17
Reactivity
Mouse
Applications
FC/FACS, WB, IHC, BL, FA
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
(Flow cytometry using the Anti-CD25 antibody 2E4 (MBS488869). Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (MBS488149; isotype control, black line) or the rabbit IgG version of 2E4 (MBS488869, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
CD25, Monoclonal Antibody (Cat# AAA488868)
Full Name
Anti-CD25 [2E4]
Gene Names
Il2ra; CD25; Il2r; Ly-43
Reactivity
Mouse
Applications
FC/FACS
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
