Immunofluorescence (IF)
(Immunofluorescence staining of fixed mouse splenocytes with anti-PD-1H antibody MH5A (MBS488594) Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of MH5A () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.)
Rabbit anti-Mouse PD-1H Monoclonal Antibody | anti-PD-1H antibody
Anti-PD-1H [MH5A]
Gene Names
Vsir; Dies1; PD-1H; VISTA; 4632428N05Rik
Reactivity
Mouse
Applications
ELISA, Immunohistochemistry
Purity
Purified antibody. Protein A affinity purified
Synonyms
PD-1H; Monoclonal Antibody; Anti-PD-1H [MH5A]; VISTA; VISR; B7-H5; B7H5; GI24; PP2135; SISP1; DD1alpha; C10orf54; chromosome 10 open reading frame 54; V-set immunoregulatory receptor; anti-PD-1H antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG, lambda
Clone Number
MH5A
Specificity
This antibody specifically binds PD-1H.
Purity/Purification
Purified antibody. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-PD-1H antibody
ELISA (EIA), Immunohistochemistry (IHC), Blocking (BL), Therapeutics
Application Notes
The antibody has been labelled with biotin and used to stain formalin-fixed tissues from wild-type C57BL/6 mice at 6 weeks of age. This showed specific staining for PD-1H in the T cell zone and marginal zone. The antibody stains P815 cells transfected with full-length PD-1H plasmid, but not control plasmid. Antibody specificity has been confirmed by ELISA. A single 200-ug dose of MH5A prevented aGVHD in mouse models, caused a reduction in accumulation and expansion of CD8+ and CD4+ T cells in spleens and livers and greatly reduced numbers of inflitrating T cells in all aGVHD target tissues examined. Few mAbs targeting a single cosignalling molecule have such a potent preventative effect (Flies D et al, 2011).
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.
PMID
21768399
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Immunofluorescence (IF)
(Immunofluorescence staining of fixed mouse splenocytes with anti-PD-1H antibody MH5A (MBS488594) Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of MH5A () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed mouse splenocytes with anti-PD-1H antibody MH5A (MBS488594) Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of MH5A () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.)
ELISA (EIA)
(ELISA of anti-VISTA antibody on VISTA-Fc fusion protein. Binding curve of the rabbit chimeric version of the anti-VISTA antibody MH5A (; blue line) to an ELISA plate coated with mouse VISTA-Fc fusion protein (Pr00164-1.9) at a concentration of 5ug/ml. A 3-fold serial dilution from 10000 to 1.7 ng/ml was performed using . For signal detection, a 1:4000 dilution of anti-rabbit IgG1 HRP (BioRad) antibody was used.)
ELISA (EIA)
(ELISA of anti-VISTA antibody on VISTA-Fc fusion protein. Binding curve of the rabbit chimeric version of the anti-VISTA antibody MH5A (; blue line) to an ELISA plate coated with mouse VISTA-Fc fusion protein (Pr00164-1.9) at a concentration of 5ug/ml. A 3-fold serial dilution from 10000 to 1.7 ng/ml was performed using . For signal detection, a 1:4000 dilution of anti-rabbit IgG1 HRP (BioRad) antibody was used.)
Related Product Information for anti-PD-1H antibody
Recombinant monoclonal antibody to PD-1H. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma MH5A.
The antibody was generated by immunising Armenian hamsters with mPD-1HIg.
The antibody was generated by immunising Armenian hamsters with mPD-1HIg.
Product Categories/Family for anti-PD-1H antibody
References
Flies D et al. Cutting Edge: A Monoclonal Antibody Specific for the Programmed Death-1 Homolog Prevents Graft-versus-Host Disease in Mouse Models J Immunol August 15, 2011, 187 (4) 1537-1541
Note on publication: Describes the generation of the antibody and characterisation of its theraputic function in models of graft-versus-host disease.
Note on publication: Describes the generation of the antibody and characterisation of its theraputic function in models of graft-versus-host disease.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,559 Da
NCBI Official Full Name
platelet receptor Gi24 isoform 2
NCBI Official Synonym Full Names
V-set immunoregulatory receptor
NCBI Official Symbol
Vsir
NCBI Official Synonym Symbols
Dies1; PD-1H; VISTA; 4632428N05Rik
NCBI Protein Information
platelet receptor Gi24
UniProt Protein Name
V-type immunoglobulin domain-containing suppressor of T-cell activation
UniProt Gene Name
Vsir
UniProt Entry Name
VISTA_MOUSE
Uniprot Description
VISTA: May stimulate MMP14-mediated MMP2 activation
Protein type: Membrane protein, integral
Cellular Component: external side of plasma membrane; intercellular junction; plasma membrane
Molecular Function: protein binding
Biological Process: BMP signaling pathway; negative regulation of T cell cytokine production; positive regulation of BMP signaling pathway; stem cell differentiation
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Product Notes
The PD-1H vsir (Catalog #AAA488595) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PD-1H [MH5A] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PD-1H can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Blocking (BL), Therapeutics. The antibody has been labelled with biotin and used to stain formalin-fixed tissues from wild-type C57BL/6 mice at 6 weeks of age. This showed specific staining for PD-1H in the T cell zone and marginal zone. The antibody stains P815 cells transfected with full-length PD-1H plasmid, but not control plasmid. Antibody specificity has been confirmed by ELISA. A single 200-ug dose of MH5A prevented aGVHD in mouse models, caused a reduction in accumulation and expansion of CD8+ and CD4+ T cells in spleens and livers and greatly reduced numbers of inflitrating T cells in all aGVHD target tissues examined. Few mAbs targeting a single cosignalling molecule have such a potent preventative effect (Flies D et al, 2011). Researchers should empirically determine the suitability of the PD-1H vsir for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PD-1H, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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