Human Vitronectin (VTN) ELISA Kit | VTN elisa kit

Human Vitronectin (VTN) ELISA Kit

Cat. Num. AAA727080

• Gene Names: VTN; VN; V75; VNT
• Reactivity: Human
• Synonyms: Vitronectin (VTN); Human Vitronectin (VTN) ELISA Kit; VTN elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 10-250ng/mL
• Sensitivity: 1.0ng/mL
• Intended Uses: This VTN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human VTN. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VTN elisa kit

Principle of the assay: VTN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-VTN antibody and an VTN-HRP conjugate. The assay sample and buffer are incubated together with VTN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the VTN concentration since VTN from samples and VTN-HRP conjugate compete for the anti-VTN antibody binding site. Since the number of sites is limited, as more sites are occupied by VTN from the sample, fewer sites are left to bind VTN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VTN concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 88853069
• NCBI GeneID: 7448
• NCBI Accession #: NP_000629.3
• NCBI GenBank Nucleotide #: NM_000638.3
• Molecular Weight: 54,306 Da
• NCBI Official Full Name: vitronectin
• NCBI Official Synonym Full Names: vitronectin
• NCBI Official Symbol: VTN
• NCBI Official Synonym Symbols: VN; V75; VNT
• NCBI Protein Information: vitronectin; epibolin; S-protein; somatomedin B; complement S-protein; serum spreading factor; serum-spreading factor
• UniProt Protein Name: Vitronectin
• Protein Family: Vitronectin
• UniProt Gene Name: VTN
• UniProt Synonym Gene Names: VN
• UniProt Entry Name: VTNC_HUMAN

NCBI Description

The protein encoded by this gene is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. [provided by RefSeq, Jul 2008]

Uniprot Description

VTN: Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.

Protein type: Secreted; Motility/polarity/chemotaxis; Cell adhesion; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q11

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; extracellular region

Molecular Function: heparin binding; integrin binding; protein binding; extracellular matrix binding; scavenger receptor activity; polysaccharide binding

Biological Process: receptor-mediated endocytosis; extracellular matrix organization and biogenesis; positive regulation of protein binding; cell-matrix adhesion; negative regulation of blood coagulation; positive regulation of smooth muscle cell migration; positive regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of receptor-mediated endocytosis; positive regulation of peptidyl-tyrosine phosphorylation; regulation of complement activation; innate immune response; cell adhesion mediated by integrin; immune response; cell adhesion