Flow Cytometry (FC/FACS)
(Flow-cytometry using the Anti-IL-9 antibody D9302C12 (MBS488651). Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of D9302C12 (MBS488651, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.)
anti-Mouse IL-9 Monoclonal Antibody | anti-IL-9 antibody
Anti-IL-9 [D9302C12]
Gene Names
Il9; P40; Il-9
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Purity
Purified antibody. Protein A affinity purified
Synonyms
IL-9; Monoclonal Antibody; Anti-IL-9 [D9302C12]; Interleukin-9; IL9; Cytokine P40; T-cell growth factor P40; anti-IL-9 antibody
Host
Hamster (Armenian)
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Hamster (Armenian) IgG, kappa
Clone Number
D9302C12
Specificity
This antibody is specific for murine IL-9.
Purity/Purification
Purified antibody. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-IL-9 antibody
Blocking (BL), Flow Cytometry (FC/FACS)
Application Notes
In vitro, this antibody has inhibitory effects on IL-9 bioactivity, and inhibits the differentiation of Th17 cells and consequent production of IL-17 (Li et al, 2010). This antibody is also able to neutralise IL-9 in vivo (Li et al, 2010). Administration of this antibody suppresses acute experimental autoimmune encephalomyelitis (EAE), with an observed reduction in MOG-specific Th1 and Th17 cells in the periphery and the CNS (Li et al, 2010).
PMID
7884304
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Flow Cytometry (FC/FACS)
(Flow-cytometry using the Anti-IL-9 antibody D9302C12 (MBS488651). Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of D9302C12 (MBS488651, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.)
Flow Cytometry (FC/FACS)
(Flow-cytometry using the Anti-IL-9 antibody D9302C12 (MBS488651). Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of D9302C12 (MBS488651, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.)
Related Product Information for anti-IL-9 antibody
Recombinant monoclonal antibody to IL-9. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma D9302C12.
This antibody was raised by immunising Armenian hamsters with Baculovirus-expressed, recombinant murine IL-9.
This antibody was raised by immunising Armenian hamsters with Baculovirus-expressed, recombinant murine IL-9.
Product Categories/Family for anti-IL-9 antibody
References
Renauld et al. Interleukin?9 and its receptor: involvement in mast cell differentiation and T cell oncogenesis J Leukoc Biol. 1995 57(3):353-60
Note on publication: Describes the original generation of this antibody.
Note on publication: Describes the original generation of this antibody.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
16,075 Da
NCBI Official Full Name
interleukin-9
NCBI Official Synonym Full Names
interleukin 9
NCBI Official Symbol
Il9
NCBI Official Synonym Symbols
P40; Il-9
NCBI Protein Information
interleukin-9; T-cell growth factor P40; cytokine P40
UniProt Protein Name
Interleukin-9
UniProt Gene Name
Il9
UniProt Synonym Gene Names
IL-9
UniProt Entry Name
IL9_MOUSE
Uniprot Description
IL9: Supports IL-2 independent and IL-4 independent growth of helper T-cells. Belongs to the IL-7/IL-9 family.
Protein type: Cytokine; Secreted; Secreted, signal peptide
Cellular Component: extracellular space; extracellular region
Molecular Function: growth factor activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interleukin-9 receptor binding; cytokine activity
Biological Process: immune response; positive regulation of cell growth; positive regulation of interleukin-5 biosynthetic process
Research Articles on IL-9
Similar Products
Product Notes
The IL-9 il9 (Catalog #AAA488650) is an Antibody produced from Hamster (Armenian) and is intended for research purposes only. The product is available for immediate purchase. The Anti-IL-9 [D9302C12] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IL-9 can be used in a range of immunoassay formats including, but not limited to, Blocking (BL), Flow Cytometry (FC/FACS). In vitro, this antibody has inhibitory effects on IL-9 bioactivity, and inhibits the differentiation of Th17 cells and consequent production of IL-17 (Li et al, 2010). This antibody is also able to neutralise IL-9 in vivo (Li et al, 2010). Administration of this antibody suppresses acute experimental autoimmune encephalomyelitis (EAE), with an observed reduction in MOG-specific Th1 and Th17 cells in the periphery and the CNS (Li et al, 2010). Researchers should empirically determine the suitability of the IL-9 il9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-9, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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