Rat anti-Mouse CD96 Monoclonal Antibody | anti-CD96 antibody

Anti-CD96 [6A6]

Cat. Num. AAA488665

• Gene Names: Cd96; Tactile; 1700109I12Rik
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay
• Purity: Purified antibody. Protein A affinity purified
• Synonyms: CD96; Monoclonal Antibody; Anti-CD96 [6A6]; TACTILE; T-cell surface protein tactile; T cell-activated increased late expression protein; anti-CD96 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rat IgG2a, lambda
• Clone Number: 6A6
• Specificity: This antibody is specific for murine CD96, and recognizes an epitope in the first Ig domain.
• Purity/Purification: Purified antibody. Protein A affinity purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CD96 antibody: Blocking (BL), Flow Cytometry (FC/FACS)
• Application Notes: This antibody blocks murine CD96-CD155 interaction, and has been shown to bind to the first Ig domain of CD96 and compete with CD155 binding (Roman Aguilera et al, 2018). This antibody displays significant anti-metastatic activity in four experimental lung metastases models and one spontaneous tumour metastasis model (Roman Aguilera et al, 2018), with a potency greater than the anti-CD96 monoclonal antibodies 3.3 and 8B10. The binding specificity of this antibody has been determined in flow cytometric analysis of HEK-293 cells transfected with a range of CD96 Ig domain chimeric constructs (Roman Aguilera et al, 2018).
• PMID: 17971293
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

ELISA (EIA)

(ELISA of anti-CD96 antibody on CD96-Fc fusion protein. Binding curve of the rabbit chimeric version of the anti-CD96 antibody 6A6 (MBS488664; blue line) to an ELISA plate coated with mouse CD96-Fc fusion protein (Pr00230-1.9) at a concentration of 5ug/ml. A 3-fold serial dilution from 10000 to 1.7 ng/ml was performed using MBS488664. For signal detection, a 1:4000 dilution of anti-rabbit IgG1 HRP (BioRad) antibody was used.)

Related Product Information for anti-CD96 antibody

Recombinant monoclonal antibody to CD96. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 6A6.
This antibody was raised by immunising rats with murine CD96.

Product Categories/Family for anti-CD96 antibody

cell-cell adhesion; oncology

References

Seth et al. The murine pan T cell marker CD96 is an adhesion receptor for CD155 and nectin-1. Biochem Biophys Res Commun. 2007 Dec 28;364(4):959-65.
Note on publication: Describes the original generation of this antibody, and its characterisation.

NCBI and Uniprot Product Information

• NCBI GI #: 160333634
• NCBI GeneID: 84544
• NCBI Accession #: NP_115854.2
• NCBI GenBank Nucleotide #: NM_032465.2
• UniProt Accession #: Q3U0X8; Q7TMW0; Q8C9U6; Q99M67
• Molecular Weight: 67,133 Da
• NCBI Official Full Name: T-cell surface protein tactile
• NCBI Official Synonym Full Names: CD96 antigen
• NCBI Official Symbol: Cd96
• NCBI Official Synonym Symbols: Tactile; 1700109I12Rik
• NCBI Protein Information: T-cell surface protein tactile; cell surface antigen CD96; T cell activation, increased late expression; t cell-activated increased late expression protein
• UniProt Protein Name: T-cell surface protein tactile
• Protein Family: T-cell surface protein tactile
• UniProt Gene Name: Cd96
• UniProt Entry Name: TACT_MOUSE

Uniprot Description

CD96: May be involved in adhesive interactions of activated T and NK cells during the late phase of the immune response. Promotes NK cell-target adhesion by interacting with PVR present on target cells. May function at a time after T and NK cells have penetrated the endothelium using integrins and selectins, when they are actively engaging diseased cells and moving within areas of inflammation. Defects in CD96 are a cause of C syndrome (CSYN); also called Opitz trigonocephaly syndrome. This syndrome is characterized by trigonocephaly and associated anomalies, such as unusual facies, wide alveolar ridges, multiple buccal frenula, limb defects, visceral anomalies, redundant skin, psychomotor retardation and hypotonia. A chromosomal aberration involving CD96 has been found in a patient with C syndrome. Translocation t(3;18)(q13.13;q12.1). CD96 gene was located at the 3q13.13 breakpoint. Precise structural analysis around the breakpoint showed that the gene was disrupted by the translocation in exon 5, probably leading to premature termination or loss of expression of CD96 protein. No gene was detected at the chromosome 18 breakpoint. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Cellular Component: membrane; integral to membrane

Biological Process: cell adhesion