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10000 results for "Biotin Antibody" - showing 100-150


Testing Data (Formalin-fixed, paraffin-embedded human tonsil stained with biotinylated Lambda Light Chain probe followed by anti-biotin MAb (Hyb-8). Note cell membrane & cytoplasmic staining.)

Biotin, Monoclonal Antibody (Cat# AAA438338)

Full Name
Biotin (Vitamin B7 or Vitamin H)
Reactivity
All species
Applications
EIA, FC/FACS, IF, WB, IH
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB) (Figure 1. Western blot analysis of ADA using anti-ADA antibody (MBS1752027). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human A549 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human U-937 whole cell lysates, Lane 6: human U20S whole cell lysates, Lane 7: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ADA antigen affinity purified monoclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.)

ADA, Monoclonal Antibody (Cat# AAA1752027)

Full Name
Anti-ADA Antibody (monoclonal, 6D4)
Reactivity
Human
Applications
WB, IHC, FC/FACS
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunocytochemistry (ICC) (Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Erk1/2 Polyclonal Antibody . Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Erk1/2 Polyclonal Antibody at 1:100 for 12 hours at 4 degree C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Erk1/2 Antibody. (C) Composite.)

Erk1/2, Polyclonal Antibody (Cat# AAA8002000)

Full Name
Erk1/2 Antibody
Gene Names
Mapk3; p44; ERK1; ERT2; MNK1; Erk-1; Esrk1; MAPK1; Prkm3; p44erk1; p44mapk
Reactivity
Human, Mouse, Rat, Bovine, Sheep, Chicken, Fruit Fly, African Clawed Frog
Applications
WB, IHC, ICC, IF, FC/FACS
Purity
Peptide Affinity Purified
Pricing
$245/0.025 mL | $880/5x0.025 mL
Immunohistochemistry (IHC) (Immunohistochemistry with Fibronectin antibody biotin conjugated at 20X with negative controls (right). Tissue: kidney. Fixation: FFPE buffered formalin 10% conc. Antigen retrieval: Heat, Citrate pH 6.2. Pressure Cooker (top) or EDTA pH 9.5 Pressure Cooker (bottom). 2ug/ml for 1 hour @ room T. Streptavidin Conjugated HRP 10 ug/ml circa 45 min at room temp. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.)

Fibronectin, Polyclonal Antibody (Cat# AAA534416)

Full Name
Fibronectin antibody (biotin)
Gene Names
FN1; FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2
Reactivity
Human fibronectin
Applications
EIA, IHC, IP, WB
Purity
Fibronectin antibody (biotin) was purified by affinity chromatography.
Pricing
$875/0.1 mg | $3,920/5x0.1 mg
Testing Data (Formalin-paraffin tonsil stained with biotinylated Lambda Light Chain probe followed by Biotin MAb (SPM375). Note cell membrane & cytoplasmic staining.)

Biotin, Monoclonal Antibody (Cat# AAA438174)

Full Name
Biotin (Vitamin B7 or Vitamin H)
Reactivity
All species
Applications
EIA, FC/FACS, IF, WB, IH
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 . Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5000 for 1 hour at RT. Localization: Inflammatory and epithelial mucosa. Magnification: 40x. Inflammatory and epithelial mucosa.)

HSP90, Monoclonal Antibody (Cat# AAA8001863)

Full Name
HSP90 Antibody
Gene Names
HSP90AB1; HSP84; HSPC2; HSPCB; D6S182; HSP90B
Reactivity
Human, Mouse, Rat, Rabbit, Chicken, Dog, Fish, Shark, Hamster
Applications
WB, IHC, ICC, IF, IP, EIA, AM
Purity
Protein G Purified
Pricing
$440/0.2 mg | $1,760/5x0.2 mg
Western Blot (WB) (Figure 1. Western blot analysis of RANK using anti- RANK antibody (MBS1750556).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat thymus tissue lysates,Lane 2: mouse thymus tissue lysates,Lane 3: HEPG2 whole Cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- RANK antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RANK at approximately 80KD. The expected band size for RANK is at 66KD. )

RANK, Polyclonal Antibody (Cat# AAA1750556)

Full Name
Anti-RANK Picoband Antibody
Gene Names
TNFRSF11A; FEO; OFE; ODFR; OSTS; PDB2; RANK; CD265; OPTB7; TRANCER; LOH18CR1
Reactivity
Human, Mouse, Rat
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunohistochemistry (IHC) (Figure 5. IHC analysis of Laminin using anti-Laminin antibody (MBS1750839). Laminin was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Laminin Antibody (MBS1750839) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )

Laminin, Polyclonal Antibody (Cat# AAA1750839)

Full Name
Anti-Laminin Picoband Antibody
Gene Names
Lamc1; Lamb2
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg

anti-human IgG, Secondary Antibody (Cat# AAA355675)

Full Name
Mouse secondary antibody to human IgG (Fc)-Biotin
Reactivity
Human
Applications
WB, IHC, IP, EIA
Purity
This product was purified from antiserum by immunoaffinity chromatography (monoclonal).
Pricing
$225/0.1 mL | $745/5x0.1 mL
Immunohistochemistry (IHC) (Figure 1. IHC analysis of Calbindin using anti-Calbindin antibody (MBS176994). Calbindin was detected in paraffin-embedded section of Human Intestinal Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Calbindin Antibody (MBS176994) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )

Calbindin, Polyclonal Antibody (Cat# AAA176994)

Full Name
Anti-Calbindin Antibody
Gene Names
CALB1; CALB
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of beta Catenin using anti-beta Catenin antibody (MBS1750301).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human placenta tissue lysates,Lane 2: human A431 whole cell lysates,Lane 3: human SK-OV-3 whole cell lysates,Lane 4: rat heart tissue lysates,Lane 5: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-beta Catenin antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for beta Catenin at approximately 90KD. The expected band size for beta Catenin is at 85KD. )

beta Catenin, Polyclonal Antibody (Cat# AAA1750301)

Full Name
Anti-beta Catenin Picoband Antibody
Gene Names
CTNNB1; EVR7; CTNNB; MRD19; armadillo
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of PC1/3/PCSK1 using anti-PC1/3/PCSK1 antibody Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Liver Tissue LysateLane 2: Rat Thymus Tissue LysateLane 3: A549 Cell LysateLane 4: HELA Cell LysateLane 5: COLO320 Cell LysateLane 6: PANC Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PC1/3/PCSK1 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PC1/3/PCSK1 at approximately 84KD. The expected band size for PC1/3/PCSK1 is at 84KD.)

PC1/3, Polyclonal Antibody (Cat# AAA176272)

Full Name
Anti-PC1/3 antibody
Gene Names
PCSK1; PC1; PC3; NEC1; SPC3; BMIQ12
Reactivity
Reacts with: Human, rat; Predicted to work with: Mouse
Applications
WB, IHC, IF
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Mouse Anti-DNA Damage Monoclonal Antibody, Clone 15A3. Tissue: Retinal Injury Model. Species: Mouse. Primary Antibody: Mouse Anti-DNA Damage Monoclonal Antibody at 1:1000. Secondary Antibody: Alexa Fluor 594 Goat Anti-Mouse (red). Courtesy of: Dr. Rajashekhar Gangaraju, University of Indiana, Department of Ophthalmology, Eugene and Marilyn Glick Eye Institute.)

DNA/RNA Damage, Monoclonal Antibody (Cat# AAA802475)

Full Name
DNA/RNA Damage Antibody, 15A3; Anti-DNA/RNA Damage Antibody
Reactivity
All
Applications
IHC, ICC, IF, DB, IP, FCM, FA
Pricing
$255/0.025 mg
Testing Data (Staining of Con A activated rat spleen cells with Mouse anti Rat CD25: Biotin (MBS210477))

CD25, Monoclonal Antibody (Cat# AAA212961)

Full Name
MOUSE ANTI RAT CD25
Gene Names
Il2ra; IL2RAC
Applications
EIA, FC/FACS, IP
Pricing
$490/0.25 mg | $2,050/5x0.25 mg
Immunocytochemistry (ICC) (Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Ubiquitin Polyclonal Antibody . Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody at 1:100 for 12 hours at 4 degree C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Ubiquitin Antibody. (C) Composite. Heat Shocked at 42 degree C for 1h.)

Ubiquitin, Polyclonal Antibody (Cat# AAA8001999)

Full Name
Ubiquitin Antibody
Reactivity
Human, Mouse, Rat, Bovine, Monkey, Hamster, Rabbit, Guinea Pig, Dog, Sheep, Chicken, African Clawed Frog, Yeast, Fruit Fly, Fish, Rainbow Trout
Applications
WB, IHC, ICC, IF, IP
Purity
Peptide Affinity Purified
Pricing
$435/0.2 mL | $1,720/5x0.2 mL
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Rabbit Anti-Amyloid Fibrils (OC) Polyclonal Antibody (SPC-507). Tissue: Alzheimer's Disease brain. Species: Human. Fixation: Formalin fixed. Primary Antibody: Rabbit Anti-Amyloid Fibrils (OC) Polyclonal Antibody (SPC-507) at 1:5000. Secondary Antibody: Goat Anti-Rabbit ATTO 488 (green). Localization: Plaque. (A) Amyloid Fibril (OC) Antibody (SPC-507). (B) Amyloid Oligomer (A11) Antibody (SPC-506). (C) Composite. Courtesy of: Dr. Elizabeth Head, University of California, Irvine.)

Amyloid Fibrils (OC), Polyclonal Antibody (Cat# AAA805797)

Full Name
Amyloid Fibrils (OC) Antibody: Biotin
Rabbit Anti-Human Amyloid Fibrils (OC) Antibody Polyclonal
Reactivity
Human
Applications
IP, ICC, IHC, EIA, WB, DB, IF
Purity
Protein A Purified
Pricing
$460/0.1 mL | $1,860/5x0.1 mL
Immunocytochemistry (ICC) (Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36. Tissue: iPSC-derived cortical excitatory neurons. Species: Human. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody at 1:500 for Overnight. Secondary Antibody: Donkey anti-rabbit: Alexa Fluor 488 at 1:1000. Counterstain: DAPI. A) iPSC-derived neurons from non-demented control (NDC). B) iPSC-derived neurons from subject with P301L MAPT mutation. Images acquired using an automated Opera Phoenix system. Each field of view is a max projection from 10 planes of 1 um stacks.. Courtesy of: Francesco Paonessa.)

Tau, Monoclonal Antibody (Cat# AAA8005577)

Full Name
Tau Antibody (pSer202/ pThr205), Clone AH36: Biotin
Gene Names
MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
Reactivity
Human
Applications
WB, DB, ICC, IF, EIA
Purity
Affinity Purified
Pricing
$620/0.1 mg | $2,560/5x0.1 mg
Western Blot (WB) (MBS423038 (0.03ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)

calbindin D28, Antibody (Cat# AAA423038)

Full Name
Goat anti-calbindin D28 Antibody
Gene Names
CALB1; CALB; D-28K
Reactivity
Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Rat, Dog, Pig, Cow
Applications
EIA, WB, IHC
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg
Western Blot (WB) (Western blotAll lanes: MCCC1 antibody at 0.89 ug/mlLane 1: HepG-2 whole cell lysateLane 2: 293T whole cell lysateLane 3: Hela whole cell lysateLane 4: MCF7 whole cell lysateLane 5: A549 whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 80 kDaObserved band size: 80 kDa)

MCCC1, Polyclonal Antibody (Cat# AAA7044109)

Full Name
MCCC1 Antibody
Gene Names
MCCC1; MCCA; MCC-B
Reactivity
Human
Applications
EIA, WB, IHC
Purity
Antigen Affinity Purified
Pricing
$180/0.05 mL | $260/0.1 mL | $1,160/5x0.1 mL
Western Blot (WB) (Anti-SLC2A5 antibody, PA1737, Western blottingAll lanes: Anti SLC2A5 at 0.5ug/mlLane 1: Rat Kidney Tissue Lysate at 50ugLane 2: Rat Liver Tissue Lysate at 50ugLane 3: Mouse Kidney Tissue Lysate at 50ugPredicted bind size: 55KDObserved bind size: 55KD)

Glucose Transporter 5 GLUT5, Polyclonal Antibody (Cat# AAA175118)

Full Name
Polyclonal Anti-SLC2A5 Antibody
Gene Names
Slc2a5; Glut5; Slc5a; AI526984
Reactivity
Mouse, Rat
Applications
WB, IHC, IHC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Testing Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD204:FITC (MBS214937))

CD204, Monoclonal Antibody (Cat# AAA214934)

Full Name
RAT ANTI MOUSE CD204:Biotin
Gene Names
Msr1; MSR; Scvr; MRS-A; MSR-A; SR-AI; SR-AII; Scara1
Applications
FC/FACS
Pricing
$415/0.1 mg | $1,710/5x0.1 mg
Testing Data (Published customer image: R-phycoerythrin conjugated Rat anti Mouse CD18 antibody, clone YFC118.3 used for flow cytometryImage caption:The levels of CD11b and CD18 fall dramatically following incubation with Leishmania under serum dependent (SD) culture conditions. Peritoneal macrophages were selected based on forward- versus side-scatter parameters as described in Materials and Methods and Figure 1 using flow cytometry. A) Isotype control with macrophages showing delineation of the M1 marker based on the negative population. B and C represent expression of CD18 (B) or CD11b (C) before incubation with Leishmania. D and E represent expression of CD18 (D) or CD11b (E) after association with Leishmania in a SD assay. In all cases the percent positive cells within the M1 marker are identified. This Figure is a representative diagram of 4 experiments with similar findings.From: Gonçalves R, Vieira ER, Melo MN, Gollob KJ, Mosser DM, Tafuri WL. A sensitive flow cytometric methodology for studying the binding of L. chagasi to canine peritoneal macrophages. BMC Infect Dis. 2005 May 24;5:39.)

CD18, Monoclonal Antibody (Cat# AAA213057)

Full Name
RAT ANTI HUMAN CD18
Gene Names
ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
Reactivity
Dog, Guinea Pig
Applications
FC/FACS, IP
Pricing
$475/0.2 mg | $1,995/5x0.2 mg
Western Blot (WB) (Figure 1. Western blot analysis of Aquaporin 1 using anti- Aquaporin 1 antibody (MBS175198). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Kidney Tissue Lysate, Lane 2: Rat Lung Tissue Lysate, Lane 3: SMMC Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Aquaporin 1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Aquaporin 1 at approximately 28KD. The expected band size for Aquaporin 1 is at 28KD. )

Aquaporin 1, Polyclonal Antibody (Cat# AAA175198)

Full Name
Anti-Aquaporin 1 antibody
Gene Names
AQP1; CO; CHIP28; AQP-CHIP
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IHC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of CHRNA5 using anti-CHRNA5 antibody (MBS178666). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: rat skeletal muscle tissue lysates, lane 2: HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHRNA5 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CHRNA5 at approximately 53KD. The expected band size for CHRNA5 is at 53KD. )

CHRNA5, Polyclonal Antibody (Cat# AAA178666)

Full Name
Anti-CHRNA5 Antibody
Gene Names
CHRNA5; LNCR2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunohistochemistry (IHC) (Figure 1. IHC analysis of BDNF using anti-BDNF antibody (MBS176974). BDNF was detected in paraffin-embedded section of Rat Brain Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BDNF Antibody (MBS176974) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )

BDNF, Polyclonal Antibody (Cat# AAA176974)

Full Name
Anti-BDNF Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of CD31 using anti-CD31 antibody (MBS176761).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.lane 1: recombinant human CD31 protein 0.5ng.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD31 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD31 at approximately 39KD. The expected band size for CD31 is at 39KD. )

CD31, Polyclonal Antibody (Cat# AAA176761)

Full Name
Anti-CD31 Antibody
Gene Names
PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
Reactivity
Human
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of Periostin using anti-Periostin antibody (MBS1750633).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat gaster tissue lysates,Lane 2: rat lung tissue lysates,Lane 3: rat NRK whole cell lysates,Lane 4: mouse gaster tissue lysates,Lane 5: mouse intestine tissue lysates,Lane 6: mouse lung tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Periostin antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Periostin at approximately 93KD. The expected band size for Periostin is at 93KD. )

Periostin, Polyclonal Antibody (Cat# AAA1750633)

Full Name
Anti-Periostin Picoband Antibody
Gene Names
Postn; Plf
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of Ataxin 3 using anti-Ataxin 3 antibody (PB9423). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate Lane 2: COLO320 Whole Cell Lysate Lane 3: HELA Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ataxin 3 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Ataxin 3 at approximately 42KD. The expected band size for Ataxin 3 is at 42KD.)

Ataxin 3, Polyclonal Antibody (Cat# AAA178269)

Full Name
Anti-Ataxin 3 Antibody
Gene Names
ATXN3; AT3; JOS; MJD; ATX3; MJD1; SCA3
Reactivity
Human, Rat
Applications
WB, IHC, ICC, FC
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of Lamin B1 using anti-Lamin B1 antibody (MBS178376).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: U87 Whole Cell Lysate,Lane 2: PC-12 Whole Cell Lysate,Lane 3: NIH3T3 Whole Cell Lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lamin B1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Lamin B1 at approximately 67KD. The expected band size for Lamin B1 is at 67KD. )

Lamin B1, Polyclonal Antibody (Cat# AAA178376)

Full Name
Anti-Lamin B1 Antibody
Gene Names
LMNB1; LMN; ADLD; LMN2; LMNB
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunohistochemistry (IHC) (Sample Type: Mouse spleenSample Type: Mouse Spleen frozen with positive lymphocytes/ HPF (x400)Primary Dilution: 1:100Secondary Antibody: Vector Lab Goat Biotin-Conjugated Anti-Rabbit IgGSecondary Dilution: 1:100Image Submitted By: Atsushi Kawabata at Kansas State University )

FOXP3, Polyclonal Antibody (Cat# AAA3201135)

Full Name
FOXP3 antibody - N-terminal region
Gene Names
FOXP3; JM2; AIID; IPEX; PIDX; XPID; DIETER
Reactivity
Tested Reactivity: Human, Mouse; (Predicted Reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat, Sheep)
Applications
IHC, WB
Purity
Protein A purified
Pricing
$390/0.1 mL | $1,750/5x0.1 mL
Western Blot (WB) (Figure 1. Western blot analysis of IL17 using anti-IL17 antibody (MBS1750396).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Jurkat cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL17 at approximately 27KD. The expected band size for IL17 is at 17KD. )

IL17, Polyclonal Antibody (Cat# AAA1750396)

Full Name
Anti-IL17 Picoband Antibody
Gene Names
Il17a; Il17; Ctla8; IL-17; Ctla-8; IL-17A
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Testing Data (Immunofluoescence staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody , red in A and Mouse anti Rat CD8 , green in B. C is the merged image with nuclei counter-stained in blue using DAPI. Low power)

CD11b, Monoclonal Antibody (Cat# AAA210479)

Full Name
MOUSE ANTI RAT CD11b:Biotin
Gene Names
ITGAM; CD11B
Applications
FC/FACS
Pricing
$425/0.1 mg | $1,755/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of MVP using anti-MVP antibody (MBS1750453).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human placenta tissue lysates,Lane 2: human HepG2 whole cell lysates,Lane 3: human A549 whole cell lysates,Lane 4: human PANC-1 whole cell lysates,Lane 5: human SGC-7901 whole cell lysates,Lane 6: human MDA-MB-231 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MVP antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MVP at approximately 99KD. The expected band size for MVP is at 99KD. )

MVP, Polyclonal Antibody (Cat# AAA1750453)

Full Name
Anti-MVP Picoband antibody
Gene Names
MVP; LRP; VAULT1
Reactivity
Human, Mouse, Rat
Applications
EIA, FC/FACS, IHC, ICC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of GLO1 using anti-GLO1 antibody (MBS1750700).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human placenta tissue lysates,Lane 3: human MCF-7 whole cell lysates,Lane 4: human COLO-320 whole cell lysates,Lane 5: human 22RV1 whole cell lysates,Lane 6: human HepG2 whole cell lysates,Lane 7: human A431 whole cell lysates,Lane 8: human U-937 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLO1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GLO1 at approximately 21KD. The expected band size for GLO1 is at 21KD. )

GLO1/Glyoxalase I, Polyclonal Antibody (Cat# AAA1750700)

Full Name
Anti-GLO1/Glyoxalase I Picoband antibody
Gene Names
GLO1; GLYI; GLOD1; HEL-S-74
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Pancreas stained with Mitochondria Monoclonal Antibody (AE-1).)

FITC (Fluorescein Isothiocyanate), Monoclonal Antibody (Cat# AAA4380398)

Full Name
FITC (Fluorescein Isothiocyanate)
Reactivity
All species
Applications
FC/FACS, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB) (Figure 1. Western blot analysis of NFIB/NF1B2 using anti-NFIB/NF1B2 antibody (MBS1750665). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates,Lane 2: rat PC-12 whole cell lysates,Lane 3: mouse lung tissue lysates,Lane 4: mouse ovary tissue lysates,Lane 5: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFIB/NF1B2 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NFIB/NF1B2 at approximately 50KD. The expected band size for NFIB/NF1B2 is at 47KD. )

NFIB/NF1B2, Polyclonal Antibody (Cat# AAA1750665)

Full Name
Anti-NFIB/NF1B2 Picoband antibody
Gene Names
NFIB; CTF; NF1-B; NFI-B; NFIB2; NFIB3; NF-I/B; NFI-RED; HMGIC/NFIB
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
IHC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Flow Cytomerty (FC) (Flow cytometric detection of Calprotectin with MBS584343. The green and black line represent the isotype control in 2 concentrations, the pink line the secondary antibody only and the green and brown line MBS584343 in a concentration of 1 and 3 ul/200000 cells.)

Calprotectin, Monoclonal Antibody (Cat# AAA584259)

Full Name
Calprotectin, Human, mAb 27E10, Biotin
Reactivity
Mouse: No; Rhesus Monkey: Yes (subpopulations of macrophages)
Applications
IHC-F, IF, FC, IA, IP, WB
Purity
Protein G
Pricing
$535/0.05 mg | $2,115/5x0.05 mg
Western Blot (WB) (Figure 1. Western blot analysis of Mesothelin using anti-Mesothelin antibody (MBS178354).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.Lane 1: Recombinant Human Mesothelin Protein 0.5ng.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mesothelin antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Mesothelin at approximately 43KD. The expected band size for Mesothelin is at 43KD. )

Mesothelin, Polyclonal Antibody (Cat# AAA178354)

Full Name
Anti-Mesothelin Antibody
Gene Names
MSLN; MPF; SMRP
Reactivity
Human
Applications
WB, IHC, EIA, FC, ICC
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Testing Data (Published customer image: NKG2D-dependent infiltration of CD8+T cells into tumors. Tumors were collected from mice that had received one of the four standard treatments: control DNA, Dox plus control DNA, IL-12, Dox plus IL-12 (n = 3 per treatment group). (A) Infiltration of NKG2D-positive cells into tumors. Northern blot analysis was performed to detect NKG2D expression in tumors. Ribosomal RNA was used to confirm equal loading among samples. (B) NKG2D/CD8 -positive cells in tumor sections by treatment received. Frozen tumor sections were stained with biotin anti-mouse NKG2D, anti-mouse CD8, or corresponding isotype control antibodies, then with streptavidin-conjugated Alexa fluor 594 or Alexa fluor 488 secondary antibodies. Data shown are representative of three independent experiments. The scale bar is equivalent to 100 um.From: CD8+T cell-specific induction of NKG2D receptor by doxorubicin plus interleukin-12 and its contribution to CD8+T cell accumulation in tumors. Hu J, Zhu S, Xia X, Zhang L, Kleinerman ES, Li S. Mol Cancer. 2014 Feb 24;13:34.)

CD8 ALPHA, Monoclonal Antibody (Cat# AAA214268)

Full Name
RAT ANTI MOUSE CD8:Low Endotoxin
Gene Names
Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
Applications
FC/FACS, IF
Pricing
$745/0.5 mg | $3,200/5x0.5 mg
Testing Data (Staining of mouse peripheral blood lymphocytes Rat anti Mouse CD16/32: Alexa Fluor 488 (MBS212682A488))

CD16/CD32, Monoclonal Antibody (Cat# AAA213784)

Full Name
RAT ANTI MOUSE CD16/CD32:RPE
Gene Names
Fcgr2b; CD32; Fcgr2; Fcr-2; Fcr-3; Ly-17; LyM-1; Lym-1; fcRII; FcgRII; Fcgr2a; Ly-m20; AI528646; Fc[g]RII; F630109E10Rik
Applications
FC/FACS
Pricing
$500/100 Tests | $2,090/5x100 Tests
Western Blot (WB) (Figure 1. Western blot analysis of SAE2/UBA2 using anti-SAE2/UBA2 antibody (MBS1750853).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human placenta tissue lysates,Lane 3: human MCF-7 whole cell lysates,Lane 4: human A549 whole cell lysates,Lane 5: human SK-OV-3 whole cell lysates,Lane 6: human 22RV1 whole cell lysates,Lane 7: human A431 whole cell lysates,Lane 8: human COLO-320 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAE2/UBA2 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SAE2/UBA2 at approximately 90KD. The expected band size for SAE2/UBA2 is at 71KD. )

SAE2/UBA2, Polyclonal Antibody (Cat# AAA1750853)

Full Name
Anti-SAE2/UBA2 Picoband antibody
Gene Names
UBA2; ARX; SAE2; HRIHFB2115
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Anti- Surfactant protein D Picoband antibody, MBS178061, Western blottingAll lanes: Anti Surfactant protein D (MBS178061) at 0.5ug/mlLane 1: Rat Lung Tissue Lysate at 50ugLane 2: Rat Brain Tissue Lysate at 50ugLane 3: PANC Whole Cell Lysate at 40ugPredicted bind size: 38KDObserved bind size: 38KD)

Surfactant protein D, Polyclonal Antibody (Cat# AAA178061)

Full Name
Anti-Surfactant protein D Antibody
Gene Names
SFTPD; SP-D; PSP-D; SFTP4; COLEC7
Reactivity
Human, Rat
Applications
WB, IHC, EIA
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of Serum Amyloid P using anti-Serum Amyloid P antibody (MBS1750337).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human placenta tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Serum Amyloid P antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Serum Amyloid P at approximately 25KD. The expected band size for Serum Amyloid P is at 25KD. )

Serum Amyloid P, Polyclonal Antibody (Cat# AAA1750337)

Full Name
Anti-Serum Amyloid P Picoband antibody
Gene Names
APCS; SAP; PTX2; HEL-S-92n
Reactivity
Human
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Testing Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen:FITC (MBS216414))

F4/80, Monoclonal Antibody (Cat# AAA219371)

Full Name
RAT ANTI MOUSE F4/80
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
FC, RE, IP, IF, RIA, EM
Pricing
$575/2 mL | $2,445/5x2 mL
Western Blot (WB) (Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293) lysate showing detection of Ubiquitin protein using Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119). Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119) at 1:1000.)

Ubiquitin, Polyclonal Antibody (Cat# AAA806127)

Full Name
Ubiquitin Antibody: HRP
Reactivity
Human, Monkey, Mouse, Rat, Hamster, Rabbit, Guinea pig, Bovine, Pig, Canine, Sheep, Chicken, Xenopus, Yeast, Drosophila, Fish (Rainbow trout)
Applications
WB, IP, IF
Pricing
$505/0.2 mL | $2,060/5x0.2 mL
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6. Tissue: Retinal Injury Model. Species: Mouse. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody at 1:1000. Secondary Antibody: Alexa Fluor 594 Goat Anti-Mouse (red). Courtesy of: Dr. Rajashekhar Gangaraju, University of Indiana, Department of Ophthalmology, Eugene and Marilyn Glick Eye Institute.)

Nitrotyrosine, Monoclonal Antibody (Cat# AAA802763)

Full Name
Nitrotyrosine Antibody: Alkaline Phosphatase
Reactivity
Human, Mouse, Rat, Dog
Applications
WB, EIA, IHC, IP, IF
Pricing
$485/0.1 mg | $1,960/5x0.1 mg
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody, Clone S112B-14. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear expression in the epidermis and hair follicles.)

Kir2.1, Monoclonal Antibody (Cat# AAA802253)

Full Name
Kir2.1 Antibody: Biotin
Gene Names
Kcnj2; IRK1; Kcnf1; Kir2.1
Reactivity
Mouse, Rat
Applications
WB, ICC, IF
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Immunohistochemistry (IHC) (Figure 2. IHC analysis of Xanthine Oxidase using anti-Xanthine Oxidase antibody (MBS1750725). Xanthine Oxidase was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Xanthine Oxidase Antibody (MBS1750725) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )

Xanthine Oxidase, Polyclonal Antibody (Cat# AAA1750725)

Full Name
Anti-Xanthine Oxidase Picoband Antibody
Gene Names
XDH; XO; XOR; XAN1
Reactivity
Reacts with: Mouse, Rat
Predicted to work with: Human
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of VEGF Receptor 3 using anti-VEGF Receptor 3 antibody (MBS1750605).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human MCF-7 whole cell lysates,Lane 3: human HepG2 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGF Receptor 3 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for VEGF Receptor 3 at approximately 153KD. The expected band size for VEGF Receptor 3 is at 153KD. )

VEGF Receptor 3, Polyclonal Antibody (Cat# AAA1750605)

Full Name
Anti-VEGF Receptor 3 Picoband Antibody
Gene Names
FLT4; PCL; FLT-4; FLT41; LMPH1A; VEGFR3; VEGFR-3
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of RXRA using anti-RXRA antibody (MBS1750614).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human COLO-320 whole cell lysates,Lane 3: human A431 whole cell lysates,Lane 4: human MCF-7 whole cell lysates,Lane 5: rat heart tissue lysates,Lane 6: mouse heart tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RXRA antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RXRA at approximately 51-55KD. The expected band size for RXRA is at 51KD. )

RXRA/Rxr Alpha, Polyclonal Antibody (Cat# AAA1750614)

Full Name
Anti-RXRA/Rxr Alpha Picoband antibody
Gene Names
RXRA; NR2B1
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
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