Rabbit Erk1/2 Polyclonal Antibody | anti-Erk1/2 antibody

Erk1/2 Antibody

Cat. Num. AAA8002000

• Gene Names: Mapk3; p44; ERK1; ERT2; MNK1; Erk-1; Esrk1; MAPK1; Prkm3; p44erk1; p44mapk
• Reactivity: Human, Mouse, Rat, Bovine, Sheep, Chicken, Fruit Fly, African Clawed Frog
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Peptide Affinity Purified
• Synonyms: Erk1/2; Polyclonal Antibody; Erk1/2 Antibody; Rabbit Anti-Rat Erk1/2 Polyclonal; ERK1 Antibody; ERK2 Antibody; ERT1 Antibody; ERT2 Antibody; MAP kinase1 Antibody; MAP kinase2 Antibody; MAPK1 Antibody; MAPK2 Antibody; MAPK3 Antibody; p38 Antibody; p40 Antibody; p41 Antibody; p41mapk Antibody; p42 MAPK Antibody; p44 ERK1 Antibody; p44 MAPK Antibody; PRKM1 PRKM2 Antibody; PRKM3 Antibody; anti-Erk1/2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Bovine, Sheep, Chicken, Fruit Fly, African Clawed Frog
• Clonality: Polyclonal
• Specificity: Detects ~44kda (ERK1) and ~42kDa (ERK2).
• Purity/Purification: Peptide Affinity Purified
• Form/Format: PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 380

• Applicable Applications for anti-Erk1/2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000; IHC: 1:100; ICC/IF: 1:100; FC/FACS: 1:100
• Immunogen: A 35 residue synthetic peptide, corresponding to Erk1 MAP kinase with the CGG spacer group added and the peptide coupled to KLH.
• Immunogen Species: Rat
• Conjugate: Unconjugated
• Cellular Localization: Cytoplasm, Nucleus
• Certificate of Analysis: A 1:1000 dilution was sufficient for detection of ERK1/2 in 20 ug of HeLa cell lysate by ECL immunoblot analysis.
• Preparation and Storage: Store at-20 degree C.

Immunocytochemistry (ICC)

(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Erk1/2 Polyclonal Antibody . Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Erk1/2 Polyclonal Antibody at 1:100 for 12 hours at 4 degree C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Erk1/2 Antibody. (C) Composite.)

Western Blot (WB)

(Western blot analysis of Human Cell line lysates showing detection of ERK1 protein using Rabbit Anti-ERK1 Polyclonal Antibody . Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-ERK1 Polyclonal Antibody . Tissue: Inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody at 1:25000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT.)

Immunocytochemistry (ICC)/Immunofluorescence (IF)

(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ERK1 Polyclonal Antibody . Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at-20C for 10 minutes. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody at 1:100 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Cytoplasm. Nucleus.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-ERK1 Polyclonal Antibody . Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.)

Immunocytochemistry (ICC)

(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Erk1/2 Polyclonal Antibody . Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Erk1/2 Polyclonal Antibody at 1:100 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Erk1/2 Antibody. (C) Composite.)

Related Product Information for anti-Erk1/2 antibody

The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase (1). They are both located in the cytosol and mitochondria (2). While the role of cytosol ERK1/2 is well studied and involved in multiple cellular functions (2), the role of mitochondrial ERK1/2 remains poorly understood. Both ERK 1 and 2 are activated by MEK1 or MEK2, by dual phosphorylation of a threonine and tyrosine residue in the activation loop (TEY motif) (1, 3). Either phosphorylation alone can induce an electrophoretic mobility shift, but both are required for activation of the kinase. This dual phosphorylation is efficiently detected by phosphorylation state-specific antibody directed to the pTEpY motif. Once activated, MAP kinases phosphorylate a broad spectrum of substrates, including cytoskeletal proteins, translation regulators, transcription factors, and the Rsk family of protein kinases (4). ERK1/2 activation is generally thought to confer a survival advantage to cells (5); however there is increasing evidence that suggests that the activation of ERK1/2 also contributes to cell death under certain conditions (5). ERK1/2 also is activated in neuronal and renal epithelial cells upon exposure to oxidative stress and toxicants or deprivation of growth factors, and inhibition of the ERK pathway blocks apoptosis (5).

Product Categories/Family for anti-Erk1/2 antibody

Cell Signaling; Post-translational Modifications; Phosphorylation; Neuroscience; Neurodegeneration; Alzheimer's Disease

References

1. Boulton TG. et al. (1991) Biochemistry. 30(1):278-86. 2. Yoon S., and Seger R. (2006) Growth Factors 24:21-44. 3. Wolf G. (2005) Antioxid Redox Signal 7:1337-1345. 4. Chuerland D., Marmor G., Shainskaya A. and Seger R. (2008) J Biol Chem. 5. Zhuang S., and Schnellmann R.G. (2006) J Pharmacol Exp Ther 319:991-997.

NCBI and Uniprot Product Information

• NCBI GI #: 8393331
• NCBI GeneID: 50689
• NCBI Accession #: NP_059043.1
• NCBI GenBank Nucleotide #: NM_017347.2
• UniProt Accession #: P21708
• NCBI Official Full Name: mitogen-activated protein kinase 3
• NCBI Official Synonym Full Names: mitogen activated protein kinase 3
• NCBI Official Symbol: Mapk3
• NCBI Official Synonym Symbols: p44; ERK1; ERT2; MNK1; Erk-1; Esrk1; MAPK1; Prkm3; p44erk1; p44mapk
• NCBI Protein Information: mitogen-activated protein kinase 3
• UniProt Protein Name: Mitogen-activated protein kinase 3
• UniProt Gene Name: Mapk3
• UniProt Synonym Gene Names: Erk1; Prkm3; MAP kinase 3; MAPK 3; ERK-1; p44-MAPK

NCBI Description

kinase involved in intracellular signalling; component of Mapk signalling pathway [RGD, Feb 2006]

Uniprot Description

ERK1: a serine/threonine kinase of the GMGC group that plays a critical role in the regulation of cell growth and differentiation. ERK1 (MAPK3) and ERK2 (MAPK1) play central roles in MAPK cascades and are activated by a wide variety of extracellular signals including growth and neurotrophic factors, cytokines, hormones and neurotransmitters. Depending on the cellular context, MAPK cascades mediate diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. MAPK cascades also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine residues at the sequence T*EY* by upstream MAP kinase kinases, MEK1 and -2. Phosphorylation of both the threonine and tyrosine are required for activity. This phosphorylation causes dramatic conformational changes, which enable full activation and interaction of MAPK1/ERK2 with its substrates.

Protein type: CMGC group; EC 2.7.11.24; ERK subfamily; Kinase, protein; MAPK family; MAPK/ERK subfamily; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 1q36

Cellular Component: caveola; cytoplasm; cytoskeleton; cytosol; early endosome; extracellular exosome; focal adhesion; Golgi apparatus; late endosome; mitochondrion; nuclear envelope; nucleoplasm; nucleus; plasma membrane; protein complex; pseudopodium

Molecular Function: ATP binding; identical protein binding; MAP kinase activity; MAP kinase kinase activity; phosphatase binding; phosphotyrosine binding; protein binding; protein kinase activity; protein serine/threonine kinase activity

Biological Process: activation of MAPK activity; apoptosis; arachidonic acid metabolic process; Bergmann glial cell differentiation; BMP signaling pathway; cartilage development; caveolin-mediated endocytosis; cell surface receptor signaling pathway; cellular response to amino acid starvation; cellular response to cadmium ion; cellular response to DNA damage stimulus; cellular response to organic substance; cellular response to reactive oxygen species; DNA damage induced protein phosphorylation; ERK1 and ERK2 cascade; lipopolysaccharide-mediated signaling pathway; MAPK cascade; negative regulation of apolipoprotein binding; neural crest cell development; organ morphogenesis; outer ear morphogenesis; peptidyl-serine phosphorylation; peptidyl-tyrosine autophosphorylation; phosphorylation; positive regulation of cyclase activity; positive regulation of cytokine secretion involved in immune response; positive regulation of ERK1 and ERK2 cascade; positive regulation of gene expression; positive regulation of histone acetylation; positive regulation of histone phosphorylation; positive regulation of macrophage chemotaxis; positive regulation of metallopeptidase activity; positive regulation of protein phosphorylation; positive regulation of telomerase activity; positive regulation of telomere capping; positive regulation of telomere maintenance via telomerase; positive regulation of transcription from RNA polymerase II promoter; positive regulation of translation; positive regulation of xenophagy; protein amino acid phosphorylation; protein complex assembly; regulation of cellular pH; regulation of cytoskeleton organization; regulation of early endosome to late endosome transport; regulation of Golgi inheritance; regulation of ossification; regulation of stress-activated MAPK cascade; regulation of transcription factor activity; response to exogenous dsRNA; response to lipopolysaccharide; response to toxin; sensory perception of pain; stress-activated MAPK cascade; thymus development; thyroid gland development; transcription, DNA-dependent