Immunohistochemistry (IHC)
(Immunohistochemistry with Fibronectin antibody biotin conjugated at 20X with negative controls (right). Tissue: kidney. Fixation: FFPE buffered formalin 10% conc. Antigen retrieval: Heat, Citrate pH 6.2. Pressure Cooker (top) or EDTA pH 9.5 Pressure Cooker (bottom). 2ug/ml for 1 hour @ room T. Streptavidin Conjugated HRP 10 ug/ml circa 45 min at room temp. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.)
Rabbit Fibronectin Polyclonal Antibody | anti-FN1 antibody
Fibronectin antibody (biotin)
IHC: 1:50-1:200
IP: 1:100
WB: 1:500-1:5,000
Immunohistochemistry (IHC)
(Immunohistochemistry with Fibronectin antibody biotin conjugated at 20X with negative controls (right). Tissue: kidney. Fixation: FFPE buffered formalin 10% conc. Antigen retrieval: Heat, Citrate pH 6.2. Pressure Cooker (top) or EDTA pH 9.5 Pressure Cooker (bottom). 2ug/ml for 1 hour @ room T. Streptavidin Conjugated HRP 10 ug/ml circa 45 min at room temp. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.)
Immunohistochemistry (IHC)
(Immunohistochemistry with Fibronectin antibody biotin conjugated at 20X with negative controls (right). Tissue: kidney. Fixation: FFPE buffered formalin 10% conc. Antigen retrieval: Heat, Citrate pH 6.2. Pressure Cooker (top) or EDTA pH 9.5 Pressure Cooker (bottom). 2ug/ml for 1 hour @ room T. Streptavidin Conjugated HRP 10 ug/ml circa 45 min at room temp. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.)
Immunohistochemistry (IHC)
(Tissue: human kidney at pH9 at 20x and 40x. Fixation: formalin fixed paraffin embedded. Fibronectin antibody at 10 ug/mL for 1 h at RT. Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Fibronectin is cytoplasmic. Staining: Fibronectin as precipitated brown signal (A) with purple nuclear counterstain. With corresponding negative control (B).)
Immunohistochemistry (IHC)
(Tissue: human kidney at pH9 at 20x and 40x. Fixation: formalin fixed paraffin embedded. Fibronectin antibody at 10 ug/mL for 1 h at RT. Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Fibronectin is cytoplasmic. Staining: Fibronectin as precipitated brown signal (A) with purple nuclear counterstain. With corresponding negative control (B).)
Immunohistochemistry (IHC)
(Tissue: human kidney at pH6 at 20x and 40x. Fixation: formalin fixed paraffin embedded. Fibronectin antibody at 10 ug/mL for 1 h at RT. Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Fibronectin is cytoplasmic. Staining: Fibronectin as precipitated brown signal (A) with purple nuclear counterstain. With corresponding negative control (B).)
Immunohistochemistry (IHC)
(Tissue: human kidney at pH6 at 20x and 40x. Fixation: formalin fixed paraffin embedded. Fibronectin antibody at 10 ug/mL for 1 h at RT. Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Fibronectin is cytoplasmic. Staining: Fibronectin as precipitated brown signal (A) with purple nuclear counterstain. With corresponding negative control (B).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]
Research Articles on FN1
Similar Products
Product Notes
The FN1 fn1 (Catalog #AAA534416) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Fibronectin antibody (biotin) reacts with Human fibronectin and may cross-react with other species as described in the data sheet. AAA Biotech's Fibronectin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). ELISA: 1:5,000-1:20,000 IHC: 1:50-1:200 IP: 1:100 WB: 1:500-1:5,000. Researchers should empirically determine the suitability of the FN1 fn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fibronectin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
