Immunohistochemistry (IHC)
(Figure 2. IHC analysis of Xanthine Oxidase using anti-Xanthine Oxidase antibody (MBS1750725).
Xanthine Oxidase was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Xanthine Oxidase Antibody (MBS1750725) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Rabbit Xanthine Oxidase Polyclonal Antibody | anti-XDH antibody
Anti-Xanthine Oxidase Picoband Antibody
Predicted to work with: Human
Predicted to work with: Human
Concentration: 0.1-0.5ug/mL; Tested Species: Mouse, Rat; Predicted Species: Human
Immunohistochemistry (Paraffin-embedded Section):
Concentration: 0.5-1ug/mL; Tested Species: Mouse, Rat; Predicted Sepcies: Human; Antigen Retrieval: By Heat
Immunohistochemistry (Frozen Section):
Concentration: 0.1-1ug/mL; Tested Sepcies: Human
Immunocytochemistry:
Concentration: 0.5-1ug/mL; Testes Species: Human
Flow Cytometry:
Concentration: 1-3ug/1x106 cells; Tested Species: Human
Tested Species: In house tested species with positive results.
Predicted Species:Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by the end users.
Immunohistochemistry (IHC)
(Figure 2. IHC analysis of Xanthine Oxidase using anti-Xanthine Oxidase antibody (MBS1750725). Xanthine Oxidase was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Xanthine Oxidase Antibody (MBS1750725) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 2. IHC analysis of Xanthine Oxidase using anti-Xanthine Oxidase antibody (MBS1750725).
Xanthine Oxidase was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Xanthine Oxidase Antibody (MBS1750725) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 3. IHC analysis of Xanthine Oxidase using anti-Xanthine Oxidase antibody (MBS1750725). Xanthine Oxidase was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Xanthine Oxidase Antibody (MBS1750725) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 3. IHC analysis of Xanthine Oxidase using anti-Xanthine Oxidase antibody (MBS1750725).
Xanthine Oxidase was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Xanthine Oxidase Antibody (MBS1750725) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
)
Western Blot (WB)
(Figure 1. Western blot analysis of Xanthine Oxidase using anti-Xanthine Oxidase antibody (MBS1750725). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysate,Lane 2: mouse liver tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Xanthine Oxidase antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Xanthine Oxidase at approximately 146KD. The expected band size for Xanthine Oxidase is at 146KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of Xanthine Oxidase using anti-Xanthine Oxidase antibody (MBS1750725).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysate,Lane 2: mouse liver tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Xanthine Oxidase antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Xanthine Oxidase at approximately 146KD. The expected band size for Xanthine Oxidase is at 146KD. )
NCBI and Uniprot Product Information
NCBI Description
Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism. [provided by RefSeq, Jan 2014]
Uniprot Description
Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro).
Research Articles on XDH
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Product Notes
The XDH xdh (Catalog #AAA1750725) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Xanthine Oxidase Picoband Antibody reacts with Reacts with: Mouse, Rat Predicted to work with: Human and may cross-react with other species as described in the data sheet. AAA Biotech's Xanthine Oxidase can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). Western Blot: Concentration: 0.1-0.5ug/mL; Tested Species: Mouse, Rat; Predicted Species: Human Immunohistochemistry (Paraffin-embedded Section): Concentration: 0.5-1ug/mL; Tested Species: Mouse, Rat; Predicted Sepcies: Human; Antigen Retrieval: By Heat Immunohistochemistry (Frozen Section): Concentration: 0.1-1ug/mL; Tested Sepcies: Human Immunocytochemistry: Concentration: 0.5-1ug/mL; Testes Species: Human Flow Cytometry: Concentration: 1-3ug/1x106 cells; Tested Species: Human Tested Species: In house tested species with positive results. Predicted Species:Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by the end users. Researchers should empirically determine the suitability of the XDH xdh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Xanthine Oxidase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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