Western Blot (WB)
(MBS423038 (0.03ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
Goat calbindin D28 Antibody | anti-CALB1 antibody
Goat anti-calbindin D28 Antibody
Expected from sequence similarity: Human, Rat, Dog, Pig, Cow
Expected from sequence similarity: Human, Rat, Dog, Pig, Cow
Western blot: Approx 26kDa band observed in Human Brain (Cerebellum) lysates and in Mouse and Rat total Brain lysates (calculated MW of 30.0kDa according to NP_004920.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Parmentier et al, Eur J Biochem. 1987 Dec30;170(1-2):207-15. PMID: 3691519. Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Pig Brain and Kidney for this particular batch.
IHC: Frozen sections of Human Hypothalamus shows staining of Calbinding D2 neurons. Recommended concentration: 0.1-0.3ug/ml. Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.
Western Blot (WB)
(MBS423038 (0.03ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
Western Blot (WB)
(MBS423038 (0.03ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
Western Blot (WB)
(MBS423038 (0.1ug/ml) staining of Mouse (A) and Rat (B) Brain lysates (35?g protein in RIPA buffer). Detected by chemiluminescence.)
Western Blot (WB)
(MBS423038 (0.1ug/ml) staining of Mouse (A) and Rat (B) Brain lysates (35?g protein in RIPA buffer). Detected by chemiluminescence.)
Immunohistochemistry (IHC)
(MBS423038 (0.1ug/ml) staining of PFA-perfused cryosection of Human Hypothalamus. Antigen retrieval with citrate buffer pH 6 at 80C for 30min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.)
Immunohistochemistry (IHC)
(MBS423038 (0.1ug/ml) staining of PFA-perfused cryosection of Human Hypothalamus. Antigen retrieval with citrate buffer pH 6 at 80C for 30min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.)
Immunohistochemistry (IHC)
(MBS423038 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
(MBS423038 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. Originally described as a 27 kDa protein, it is now known to be a 28 kDa protein. It contains four active calcium-binding domains, and has two modified domains that are thought to have lost their calcium binding capability. This protein is thought to buffer entry of calcium upon stimulation of glutamate receptors. Depletion of this protein was noted in patients with Huntington disease. [provided by RefSeq, Jan 2015]
Uniprot Description
CALB1: Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. Interacts with RANBP9. Belongs to the calbindin family.
Protein type: Calcium-binding
Chromosomal Location of Human Ortholog: 8q21.3
Cellular Component: axon; cell soma; cytosol; dendrite; nucleus; synapse; terminal button
Molecular Function: calcium ion binding; protein binding; vitamin D binding; zinc ion binding
Biological Process: cytosolic calcium ion homeostasis; locomotory behavior; long-term memory; regulation of synaptic plasticity; short-term memory
Research Articles on CALB1
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Product Notes
The CALB1 calb1 (Catalog #AAA423038) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-calbindin D28 Antibody reacts with Tested: Human, Mouse, Rat Expected from sequence similarity: Human, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's calbindin D28 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 26kDa band observed in Human Brain (Cerebellum) lysates and in Mouse and Rat total Brain lysates (calculated MW of 30.0kDa according to NP_004920.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Parmentier et al, Eur J Biochem. 1987 Dec30;170(1-2):207-15. PMID: 3691519. Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Pig Brain and Kidney for this particular batch. IHC: Frozen sections of Human Hypothalamus shows staining of Calbinding D2 neurons. Recommended concentration: 0.1-0.3ug/ml. Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml. Researchers should empirically determine the suitability of the CALB1 calb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "calbindin D28, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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