Western Blot (WB)
(Figure 1. Western blot analysis of PC1/3/PCSK1 using anti-PC1/3/PCSK1 antibody Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Liver Tissue LysateLane 2: Rat Thymus Tissue LysateLane 3: A549 Cell LysateLane 4: HELA Cell LysateLane 5: COLO320 Cell LysateLane 6: PANC Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PC1/3/PCSK1 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PC1/3/PCSK1 at approximately 84KD. The expected band size for PC1/3/PCSK1 is at 84KD.)
Rabbit PC1/3 Polyclonal Antibody | anti-PCSK1 antibody
Anti-PC1/3 antibody
Concentration: 0.1-0.5 ug/ml
Tested Species: Human, Rat
Predicted Species: Mouse
Antigen Retrieval: N/A
Immunohistochemistry (Paraffin-embedded Section):
Concentration: 0.5-1 ug/ml
Tested Species: Rat
Predicted Species: Human, Mouse
Antigen Retrieval: By Heat
Immunofluorescence:
Concentration: 2 ug/ml
Tested Species: Human
Predicted Species: N/A
Antigen Retrieval: N/A
WB: The detection limit for PCSK1 is approximately 1ng/lane under reducing conditions.
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling paraffin section in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Western Blot (WB)
(Figure 1. Western blot analysis of PC1/3/PCSK1 using anti-PC1/3/PCSK1 antibody Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Liver Tissue LysateLane 2: Rat Thymus Tissue LysateLane 3: A549 Cell LysateLane 4: HELA Cell LysateLane 5: COLO320 Cell LysateLane 6: PANC Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PC1/3/PCSK1 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PC1/3/PCSK1 at approximately 84KD. The expected band size for PC1/3/PCSK1 is at 84KD.)
Western Blot (WB)
(Figure 1. Western blot analysis of PC1/3/PCSK1 using anti-PC1/3/PCSK1 antibody Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Liver Tissue LysateLane 2: Rat Thymus Tissue LysateLane 3: A549 Cell LysateLane 4: HELA Cell LysateLane 5: COLO320 Cell LysateLane 6: PANC Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PC1/3/PCSK1 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PC1/3/PCSK1 at approximately 84KD. The expected band size for PC1/3/PCSK1 is at 84KD.)
Immunohistochemistry (IHC)
(Figure 2. IHC analysis of PC1/3/PCSK1 using anti-PC1/3/PCSK1 antibody PC1/3/PCSK1 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PC1/3/PCSK1 Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Immunohistochemistry (IHC)
(Figure 2. IHC analysis of PC1/3/PCSK1 using anti-PC1/3/PCSK1 antibody PC1/3/PCSK1 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PC1/3/PCSK1 Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Immunofluorescence (IF)
(Figure 3. IF analysis of PC1/3/PCSK1 using anti-PC1/3/PCSK1 antibody PC1/3/PCSK1 was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti-PC1/3/PCSK1 Antibody overnight at 4 degree C. Biotin conjugated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using DyLight 488 Conjugated Avidin. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
Immunofluorescence (IF)
(Figure 3. IF analysis of PC1/3/PCSK1 using anti-PC1/3/PCSK1 antibody PC1/3/PCSK1 was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti-PC1/3/PCSK1 Antibody overnight at 4 degree C. Biotin conjugated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using DyLight 488 Conjugated Avidin. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an initial autocatalytic processing event in the ER to generate a heterodimer which exits the ER and sorts to subcellular compartments where a second autocatalytic even takes place and the catalytic activity is acquired. The protease is packaged into and activated in dense core secretory granules and expressed in the neuroendocrine system and brain. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. It functions in the proteolytic activation of polypeptide hormones and neuropeptides precursors. Mutations in this gene have been associated with susceptibility to obesity and proprotein convertase 1/3 deficiency. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene [provided by RefSeq, Jan 2014]
Uniprot Description
PCSK1: Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin. Belongs to the peptidase S8 family. Furin subfamily.
Protein type: EC 3.4.21.93; Protease
Chromosomal Location of Human Ortholog: 5q15-q21
Cellular Component: Golgi apparatus; extracellular space; transport vesicle
Molecular Function: serine-type endopeptidase activity
Biological Process: cellular protein metabolic process; cell-cell signaling; metabolic process; peptide hormone processing; proteolysis; regulation of insulin secretion; peptide biosynthetic process
Disease: Proprotein Convertase 1/3 Deficiency; Body Mass Index Quantitative Trait Locus 12
Research Articles on PCSK1
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Product Notes
The PCSK1 pcsk1 (Catalog #AAA176272) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PC1/3 antibody reacts with Reacts with: Human, rat; Predicted to work with: Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PC1/3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) - Paraffin-embedded Section, Immunofluroescence (IF). Western Blot: Concentration: 0.1-0.5 ug/ml Tested Species: Human, Rat Predicted Species: Mouse Antigen Retrieval: N/A Immunohistochemistry (Paraffin-embedded Section): Concentration: 0.5-1 ug/ml Tested Species: Rat Predicted Species: Human, Mouse Antigen Retrieval: By Heat Immunofluorescence: Concentration: 2 ug/ml Tested Species: Human Predicted Species: N/A Antigen Retrieval: N/A WB: The detection limit for PCSK1 is approximately 1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling paraffin section in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the PCSK1 pcsk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PC1/3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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