Testing Data
(Formalin-fixed, paraffin-embedded human tonsil stained with biotinylated Lambda Light Chain probe followed by anti-biotin MAb (Hyb-8). Note cell membrane & cytoplasmic staining.)
Mouse anti-All species Biotin Monoclonal Antibody
Biotin (Vitamin B7 or Vitamin H)
Reactivity
All species
Applications
ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunohistochemistry
Synonyms
Biotin; Monoclonal Antibody; Biotin (Vitamin B7 or Vitamin H); Vitamin B7 or Vitamin H; anti-Biotin antibody
Host
Mouse
Reactivity
All species
Clonality
Monoclonal
Isotype
IgG1
Clone Number
Hyb-8
Specificity
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Applicable Applications for anti-Biotin antibody
Confocal Microscopy, ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), In situ Nucleic Acid Hybridization, Western Blot (WB), Immunohistology (IH)
Application Notes
Confocal Microscopy; ELISA; Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); In situ Nucleic Acid Hybridization; Western Blotting; Immunohistology (Frozen & Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.) Optimal dilution for a specific application should be determined.
Localization
Depends on the target
Positive Control
Biotinylated proteins in solution or on tissues
Immunogen
Biotinylated sheep immunoglobulin
Quantity and Buffer
0.5 ml at 200ug/ml with BSA and azide
Preparation and Storage
Store at 2 to 8 degree C. Antibody is stable for 24 months.
Testing Data
(Formalin-fixed, paraffin-embedded human tonsil stained with biotinylated Lambda Light Chain probe followed by anti-biotin MAb (Hyb-8). Note cell membrane & cytoplasmic staining.)
Testing Data
(Formalin-fixed, paraffin-embedded human tonsil stained with biotinylated Lambda Light Chain probe followed by anti-biotin MAb (Hyb-8). Note cell membrane & cytoplasmic staining.)
References
1. Uccellini MB, et al. 2008. J. Immunol. 181:5875.
2. Green NM, et al. 2012. J Biol Chem. 287:39789
2. Green NM, et al. 2012. J Biol Chem. 287:39789
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Product Notes
The Biotin (Catalog #AAA438338) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Biotin (Vitamin B7 or Vitamin H) reacts with All species and may cross-react with other species as described in the data sheet. AAA Biotech's Biotin can be used in a range of immunoassay formats including, but not limited to, Confocal Microscopy, ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), In situ Nucleic Acid Hybridization, Western Blot (WB), Immunohistology (IH). Confocal Microscopy; ELISA; Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); In situ Nucleic Acid Hybridization; Western Blotting; Immunohistology (Frozen & Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.) Optimal dilution for a specific application should be determined. Researchers should empirically determine the suitability of the Biotin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Biotin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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