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118 results for " cytokine signalling" - showing 100-118
Flow Cytometry (FC/FACS)
(Flow-cytometry using the Anti-IL-17F antibody 8F5.1A9 (MBS488659). Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of 8F5.1A9 (, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.)
IL-17F, Monoclonal Antibody (Cat# AAA488659)
Full Name
Anti-IL-17F [8F5.1A9]
Gene Names
Il17f; C87042; IL-17F
Reactivity
Mouse
Applications
FC/FACS
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
(Flow-cytometry using the Anti-IL-9 antibody RM9A4 (MBS488654). Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of RM9A4 (, pink line) at a dilution of 1:100 for 1h at RT. The blue line shows unstimulated human peripheral blood leukocytes stained with . After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.)
IL-9, Monoclonal Antibody (Cat# AAA488654)
Full Name
Anti-IL-9 [RM9A4]
Gene Names
Il9; P40; Il-9
Reactivity
Mouse
Applications
EIA, FC/FACS
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
(Flow-cytometry using the Anti-IL-17A antibody MM17F3 (MBS488661). Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of MM17F3 (MBS488661, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.)
IL-17A, Monoclonal Antibody (Cat# AAA488660)
Full Name
Anti-IL-17A [MM17F3]
Gene Names
Il17a; Il17; Ctla8; IL-17; Ctla-8; IL-17A
Reactivity
Mouse
Applications
EIA, BL
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
(Flow-cytometry using the Anti-IL-17A antibody MM17F3 (MBS488661). Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of MM17F3 (, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.)
IL-17A, Monoclonal Antibody (Cat# AAA488661)
Full Name
Anti-IL-17A [MM17F3]
Gene Names
Il17a; Il17; Ctla8; IL-17; Ctla-8; IL-17A
Reactivity
Mouse
Applications
EIA, BL
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed mouse splenocytes with anti-TIM-2 antibody RMT2-14 (MBS488628) Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips stained with the chimeric rabbit IgG version of RMT2-14 (MBS488628) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488628, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
TIM-2, Monoclonal Antibody (Cat# AAA488629)
Full Name
Anti-TIM-2 [RMT2-14]
Gene Names
Timd2; G9D3; Tim2; TIM-2; C78111; AA816106; AU019358; AU019662
Reactivity
Mouse
Applications
FC/FACS, WB, IHC, BL, FA
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Western Blot using anti-CD98 antibody RL388 (MBS488662). Mouse fetal liver lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric rabbit IgG version of RL388 (MBS488662) at 0.05ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
CD98, Monoclonal Antibody (Cat# AAA488663)
Full Name
Anti-CD98 [RL388]
Gene Names
Slc3a2; 4F2; Cd98; Ly10; Mdu1; 4F2HC; Ly-10; NACAE; Ly-m10; Mgp-2hc; AI314110
Reactivity
Mouse
Applications
IP, FC/FACS
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed mouse splenocytes with anti-TIM-2 antibody RMT2-14 (MBS488628) Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips stained with the chimeric rabbit IgG version of RMT2-14 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
TIM-2, Monoclonal Antibody (Cat# AAA488628)
Full Name
Anti-TIM-2 [RMT2-14]
Gene Names
Timd2; G9D3; Tim2; TIM-2; C78111; AA816106; AU019358; AU019662
Reactivity
Mouse
Applications
FC/FACS, WB, IHC, BL, FA
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Western Blot using anti-CD98 antibody RL388 (MBS488662). Mouse fetal liver lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric rabbit IgG version of RL388 () at 0.05ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
CD98, Monoclonal Antibody (Cat# AAA488662)
Full Name
Anti-CD98 [RL388]
Gene Names
Slc3a2; 4F2; Cd98; Ly10; Mdu1; 4F2HC; Ly-10; NACAE; Ly-m10; Mgp-2hc; AI314110
Reactivity
Mouse
Applications
IP, FC/FACS
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed K562 cells with anti-TLR2 antibody TL2.1 (MBS488705) Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of TL2.1 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
TLR2, Monoclonal Antibody (Cat# AAA488705)
Full Name
Anti-TLR2 [TL2.1]
Gene Names
TLR2; TIL4; CD282
Reactivity
Human
Applications
WB, FC/FACS, IP, IF, BL
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
(Flow-cytometry using the Anti-IL-17F antibody 8F5.1A9 (MBS488659). Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of 8F5.1A9 (MBS488659, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.)
IL-17F, Monoclonal Antibody (Cat# AAA488658)
Full Name
Anti-IL-17F [8F5.1A9]
Gene Names
Il17f; C87042; IL-17F
Reactivity
Mouse
Applications
FC/FACS
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
(Flow-cytometry using the Anti-IL-9 antibody MH9A4 (MBS488652). Human peripheral blood leukocytes were rested for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of MH9A4 (MBS488652, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.)
IL-9, Monoclonal Antibody (Cat# AAA488653)
Full Name
Anti-IL-9 [MH9A4]
Gene Names
IL9; P40; HP40; IL-9
Reactivity
Human
Applications
EIA, FC/FACS
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
(Flow-cytometry using the Anti-IL-9 antibody RM9A4 (MBS488654). Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of RM9A4 (MBS488654, pink line) at a dilution of 1:100 for 1h at RT. The blue line shows unstimulated human peripheral blood leukocytes stained with MBS488654. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.)
IL-9, Monoclonal Antibody (Cat# AAA488655)
Full Name
Anti-IL-9 [RM9A4]
Gene Names
Il9; P40; Il-9
Reactivity
Mouse
Applications
EIA, FC/FACS
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
(Flow-cytometry using the Anti-IL-9 antibody D9302C12 (MBS488651). Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of D9302C12 (MBS488651, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.)
IL-9, Monoclonal Antibody (Cat# AAA488650)
Full Name
Anti-IL-9 [D9302C12]
Gene Names
Il9; P40; Il-9
Reactivity
Mouse
Applications
BL, FC/FACS
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
(Flow-cytometry using the Anti-IL-9 antibody D9302C12 (MBS488651). Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of D9302C12 (, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.)
IL-9, Monoclonal Antibody (Cat# AAA488651)
Full Name
Anti-IL-9 [D9302C12]
Gene Names
Il9; P40; Il-9
Reactivity
Mouse
Applications
BL, FC/FACS
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of mouse splenocytes using anti-IL-6R antibody (MBS488648) D7715A7. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of D7715A7 (MBS488648) at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488648, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (MBS488041) followed by Alexa Fluor 488 secondary antibody.)
IL-6R, Monoclonal Antibody (Cat# AAA488649)
Full Name
Anti-IL-6R [D7715A7]
Gene Names
Il6ra; Il6r; CD126; IL-6R; IL-6RA; IL-6R-alpha
Reactivity
Mouse
Applications
BL, FC/FACS
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of mouse splenocytes using anti-IL-6R antibody (MBS488648) D7715A7. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of D7715A7 () at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (MBS488041) followed by Alexa Fluor 488 secondary antibody.)
IL-6R, Monoclonal Antibody (Cat# AAA488648)
Full Name
Anti-IL-6R [D7715A7]
Gene Names
Il6ra; Il6r; CD126; IL-6R; IL-6RA; IL-6R-alpha
Reactivity
Mouse
Applications
BL, FC/FACS
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed K562 cells with anti-TLR2 antibody TL2.1 (MBS488705) Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of TL2.1 (MBS488705) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488705, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
TLR2, Monoclonal Antibody (Cat# AAA488704)
Full Name
Anti-TLR2 [TL2.1]
Gene Names
TLR2; TIL4; CD282
Reactivity
Human
Applications
WB, FC/FACS, IP, IF, BL
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of Jurkat cells using anti-IL-9R antibody (MBS488657) AH9R2. Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of AH9R2 () at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (MBS488041) followed by Alexa Fluor 488 secondary antibody.)
IL-9R, Monoclonal Antibody (Cat# AAA488657)
Full Name
Anti-IL-9R [AH9R2]
Gene Names
IL9R; CD129; IL-9R
Reactivity
Human
Applications
IP, BL
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
