Flow Cytometry (FC/FACS)
(Flow-cytometry using the Anti-IL-9 antibody MH9A4 (MBS488652). Human peripheral blood leukocytes were rested for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of MH9A4 (MBS488652, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.)
Mouse anti-Human IL-9 Monoclonal Antibody | anti-IL-9 antibody
Anti-IL-9 [MH9A4]
Flow Cytometry (FC/FACS)
(Flow-cytometry using the Anti-IL-9 antibody MH9A4 (MBS488652). Human peripheral blood leukocytes were rested for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of MH9A4 (MBS488652, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.)
Flow Cytometry (FC/FACS)
(Flow-cytometry using the Anti-IL-9 antibody MH9A4 (MBS488652). Human peripheral blood leukocytes were rested for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of MH9A4 (MBS488652, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.)
This antibody was raised by immunising C57Bl/6 mice with human IL-9 coupled to ovalbumin.
Note on publication: Describes the original generation of this antibody, and its use in ELISA analysis.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a cytokine that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness. [provided by RefSeq, Jul 2008]
Uniprot Description
IL9: Supports IL-2 independent and IL-4 independent growth of helper T-cells. Belongs to the IL-7/IL-9 family.
Protein type: Secreted; Secreted, signal peptide; Cytokine
Chromosomal Location of Human Ortholog: 5q31.1
Cellular Component: extracellular space
Molecular Function: growth factor activity; interleukin-9 receptor binding; cytokine activity
Biological Process: positive regulation of cell proliferation; immune response; inflammatory response; positive regulation of cell growth; positive regulation of interleukin-5 biosynthetic process
Research Articles on IL-9
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Product Notes
The IL-9 il9 (Catalog #AAA488653) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-IL-9 [MH9A4] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL-9 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS). This antibody has been used in ELISA analysis to detect human IL-9 in allergen-stimulated peripheral blood mononuclear cell supernatants (Jenmalm et al, 2001) and differentiated human T helper cell supernatants (Chang et al, 2010). This antibody, when fluorochrome-conjugated, has also been used to achieve intracellular IL-9 staining, analysed using flow cytometry (Chang et al, 2010; Beriou et al, 2010; Lozano et al, 2013). Researchers should empirically determine the suitability of the IL-9 il9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-9, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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