Flow Cytometry (FC/FACS)
(Flow-cytometry using the Anti-IL-17A antibody MM17F3 (MBS488661). Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of MM17F3 (, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.)
Rabbit anti-Mouse IL-17A Monoclonal Antibody | anti-IL-17A antibody
Anti-IL-17A [MM17F3]
Gene Names
Il17a; Il17; Ctla8; IL-17; Ctla-8; IL-17A
Reactivity
Mouse
Applications
ELISA
Purity
Purified antibody. Protein A affinity purified
Synonyms
IL-17A; Monoclonal Antibody; Anti-IL-17A [MM17F3]; Interleukin-17A; IL17A; Cytotoxic T-lymphocyte-associated antigen 8; CTLA-8; anti-IL-17A antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG, kappa
Clone Number
MM17F3
Specificity
This antibody is highly specific for murine IL-17A; in ELISA analysis, no cross-reactivity with IL?17B, IL?17C, IL?17D, IL?17E or IL?17F was observed.
Purity/Purification
Purified antibody. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-IL-17A antibody
ELISA (EIA), Blocking (BL), Therapeutics
Application Notes
The specificity of this antibody has been confirmed in ELISA analysis (Uyttenhove et al, 2006). This antibody inhibits IL?17A?induced IL?6 production by a Cl1D fibroblastic cell line in vitro (Uyttenhove et al, 2006). This antibody has been shown to prevent experimental autoimmune encephalomyelitis (EAE) disease development in vivo (Uyttenhove et al, 2006), as well as protecting C57Bl/6 mice from myelin oligdendrocyte glycoprotein (MOG)-induced EAE (Uyttenhove et al, 2007). Additionally, administration of this antibody inhibits collagen-induced arthritis (CIA) in IFN-gamma receptor knockout mice (Kelchtermans et al, 2009).
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
PMID
17048276
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Flow Cytometry (FC/FACS)
(Flow-cytometry using the Anti-IL-17A antibody MM17F3 (MBS488661). Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of MM17F3 (, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.)
Flow Cytometry (FC/FACS)
(Flow-cytometry using the Anti-IL-17A antibody MM17F3 (MBS488661). Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of MM17F3 (, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.)
Related Product Information for anti-IL-17A antibody
Recombinant monoclonal antibody to IL-17A. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma MM17F3.
This antibody was raised by immunising C57Bl/6 mice with IL-17A cross-linked to ovalbumin to generate a highly immunogenic complex.
This antibody was raised by immunising C57Bl/6 mice with IL-17A cross-linked to ovalbumin to generate a highly immunogenic complex.
Product Categories/Family for anti-IL-17A antibody
References
Uyttenhove et al. Development of an anti?IL?17A auto?vaccine that prevents experimental auto?immune encephalomyelitis Eur J Immunol. 2006 Nov;36(11):2868-74
Note on publication: Describes the original generation of this antibody, and the characterisation of its IL-17A-inhibitory activity.
Note on publication: Describes the original generation of this antibody, and the characterisation of its IL-17A-inhibitory activity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,490 Da
NCBI Official Full Name
interleukin-17A
NCBI Official Synonym Full Names
interleukin 17A
NCBI Official Symbol
Il17a
NCBI Official Synonym Symbols
Il17; Ctla8; IL-17; Ctla-8; IL-17A
NCBI Protein Information
interleukin-17A; cytotoxic T-lymphocyte-associated antigen 8
UniProt Protein Name
Interleukin-17A
UniProt Gene Name
Il17a
UniProt Synonym Gene Names
Ctla8; Il17; IL-17; IL-17A; CTLA-8
UniProt Entry Name
IL17_MOUSE
Uniprot Description
IL17A: Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts. Belongs to the IL-17 family.
Protein type: Secreted, signal peptide; Secreted
Cellular Component: extracellular space; cytoplasm; extracellular region; external side of plasma membrane
Molecular Function: cytokine activity
Biological Process: positive regulation of osteoclast differentiation; positive regulation of interleukin-23 production; positive regulation of transcription from RNA polymerase II promoter; inflammatory response
Research Articles on IL-17A
Similar Products
Product Notes
The IL-17A il17a (Catalog #AAA488661) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-IL-17A [MM17F3] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IL-17A can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Blocking (BL), Therapeutics. The specificity of this antibody has been confirmed in ELISA analysis (Uyttenhove et al, 2006). This antibody inhibits IL?17A?induced IL?6 production by a Cl1D fibroblastic cell line in vitro (Uyttenhove et al, 2006). This antibody has been shown to prevent experimental autoimmune encephalomyelitis (EAE) disease development in vivo (Uyttenhove et al, 2006), as well as protecting C57Bl/6 mice from myelin oligdendrocyte glycoprotein (MOG)-induced EAE (Uyttenhove et al, 2007). Additionally, administration of this antibody inhibits collagen-induced arthritis (CIA) in IFN-gamma receptor knockout mice (Kelchtermans et al, 2009). Researchers should empirically determine the suitability of the IL-17A il17a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-17A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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